한국식품위생안전성학회지 (Journal of Food Hygiene and Safety)

  • 제39권4호
  • /
  • Pages.304-311
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  • 2024
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  • 1229-1153(pISSN)
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  • 2465-9223(eISSN)
한국식품위생안전성학회 (The Korean Society of Food Hygiene and Safety)
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식품 오염 미생물 분석을 위한 컴팩트 드라이법 평가

Evaluation of a Compact Dry Method for Enumerating Bacteria in Contaminated Foods

  • 정수진 (중앙대학교 일반대학원 식품공학부 식품생명공학과) ;
  • 한상하 (중앙대학교 일반대학원 식품공학부 식품생명공학과) ;
  • 강준구 (중앙대학교 일반대학원 식품공학부 식품안전규제과학과) ;
  • 송민수 (중앙대학교 일반대학원 식품공학부 식품안전규제과학과) ;
  • 송혜원 (중앙대학교 일반대학원 식품공학부 식품안전규제과학과) ;
  • 이하림 (중앙대학교 일반대학원 식품공학부 식품생명공학과) ;
  • 유지수 (중앙대학교 일반대학원 식품공학부 식품안전규제과학과) ;
  • 이경옥 (중앙대학교 일반대학원 식품공학부 식품생명공학과) ;
  • 하상도 (중앙대학교 일반대학원 식품공학부 식품생명공학과)
  • Soo-Jin Jung (Department of Food Science and Biotechnology, Chung-Ang University) ;
  • Sangha Han (Department of Food Science and Biotechnology, Chung-Ang University) ;
  • June Gu Kang (Department of Food Safety and Regulatory Science, Chung-Ang University) ;
  • Min Su Song (Department of Food Safety and Regulatory Science, Chung-Ang University) ;
  • Hyewon Song (Department of Food Safety and Regulatory Science, Chung-Ang University) ;
  • Harim Lee (Department of Food Science and Biotechnology, Chung-Ang University) ;
  • Jisu Yu (Department of Food Safety and Regulatory Science, Chung-Ang University) ;
  • Kyung Ok Lee (Department of Food Science and Biotechnology, Chung-Ang University) ;
  • Sang-Do Ha (Department of Food Science and Biotechnology, Chung-Ang University)
  • 투고 : 2024.07.08
  • 심사 : 2024.08.20
  • 발행 : 2024.08.30
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초록

식품공전 시험법은 식품기초규격 적부 판정, 수거검사의 적부판정, 수입식품의 적부판정 등의 판단근거가 되기 때문에 식품산업에서 매우 중요하다. 본 연구는 도시락, 밀키트, 된장에 오염된 일반세균, 대장균군, 효모, 곰팡이, 대장균을 분리하기 방법으로 사용되는 평판배지법, Petrifilm법, Compact Dry법을 이용해 각각의 세균을 분리하였고, 회수율을 비교하였다. 식품 내 자연균총(일반세균, 효모/곰팡이, 대장균군)검출은 평판배지 PCA, PDA, DCLA와 Petrifilm AC, YM, CC의 성능을 Compact Dry TC, YMR, CF와 비교하였다. 인위접종한 대장균(E. coli)의 경우는 평판배지 EMB, Petrilfilm EC, Compact Dry EC의 검출능을 비교하였다. 본 실험결과, 일반세균, 대장균군, 대장균의 검출에서 Compact Dry법은 기존의 평판배지법 및 건조배지법과 비교하여 식품 내 각각의 세균수를 계수하는데 유의적 차이를 보이지 않았다(P>0.05). 또한, 평판배지법과 Compact Dry간의 상관관계 및 Petrifilm과 Compact Dry간의 상관관계 역시 1에 가까운 높은 값을 확인하였다. Compact Dry법은 미생물 분석을 위해 배지 준비 과정이 필요 없으며, 접종 후 자체 확산이 가능하여 사용하기 쉽고, 공간을 효율적으로 사용할 수 있으므로 기존의 배지를 사용하는 방법에 비해 많은 장점을 제공하였다. 따라서 식품 중 일반세균, 대장균군, 대장균의 검출을 위한 Compact Dry법(Compact Dry TC, CF, EC)은 기존의 식품공전 상 등재된 다른 건조필름법을 대체 가능한 것으로 판단된다.

The present study aimed to compare the accuracy of the compact dry and culture plate methods for natural flora (yeasts/molds, coliforms, and total bacterial count) and artificial inoculum (Escherichia coli) in dosirak (meat-based), meal kits (seafood-based), and Doenjang (traditional food). Compact dry TC, EC, CF, and YMR were compared with culture plate methods using a suitable medium for each bacterium. The total bacterial count, coliforms, yeasts/molds, and E. coli were assessed with 3M Petrifilm (aerobic bacterial, coliform, yeast/mold, and E. coli count plates) using culture plate methods. Analysis of the recovery rates of target microorganisms in the three food samples showed that the compact dry method for total bacteria, coliforms, and E. coli exhibited recovery capabilities equivalent to those of the culture plate and Petrifilm methods, with no significant differences (P>0.05). Overall, compact dry TC, CF, and EC showed a good correlation between the methods used in this study, indicating rapid and convenient microbial enumeration by saving time and requiring less space.

키워드

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