Herbal Formula Science (대한한의학방제학회지)

The Korean Medicine Society for the Herbal Formula Study (대한한의학방제학회)
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Effects of Polysaccharide (Polycan) derived from Black Yeast in TNF-α-induced Inflammation in the Intestinal Epithelial Cells and Loperamide-induced Constipation Models

흑효모 유래 Polycan의 TNF-α 유도 장 상피세포 염증 및 Loperamide 유도 변비 모델에 미치는 효과

  • Young Suk Kim (Glucan Co. Ltd.) ;
  • Bon Hwa Ku (Glucan Co. Ltd.) ;
  • Min Jeong Cho (Glucan Co. Ltd.) ;
  • Jung Hee Kwon (NPChemBio Inc.) ;
  • Seon Min Lee (Gyeongnam Biohealth Research Center, Gyeongnam Branch Institute, Korea Institute of Toxicology) ;
  • Tae Woo Oh (Korean Medicine (KM)-Application Center, Korea Institute of Oriental Medicine (KIOM))
  • 김영숙 ((주)글루칸) ;
  • 구본화 ((주)글루칸) ;
  • 조민정 ((주)글루칸) ;
  • 권정희 ((주)엔피켐바이오) ;
  • 이선민 (안전성평가연구소 경남분소 경남바이오헬스연구지원센터) ;
  • 오태우 (한국한의학연구원 한의기술응용센터)
  • Received : 2024.08.20
  • Accepted : 2024.08.30
  • Published : 2024.08.31
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Abstract

Objective : This study was conducted to investigate the anti-inflammatory and laxative effects of Polycan in TNF-α-treated HT-29 intestinal epithelial cells and loperamide-induced constipation in vivo models, respectively. Methods : To evaluate the anti-inflammatory effects of Polycan, HT-29 cells were treated with TNF-α in the presence or absence of Polycan. IL-8 production was measured by enzyme-linked immunosorbent assay (ELISA). MAPK phosphorylation, nuclear translocation of NF-κB, and phosphorylation of IκB were assessed by Western blot analysis. To investigate the laxative effects of Polycan, 6-week-old SD rats (8 female rats per group) were orally administered Polycan or Chicory Fiber as a positive control for 4 weeks, and constipation was induced with loperamide treatment for 10 days before sacrifice. One day before sacrifice, a charcoal meal was administered to evaluate intestinal transit times. The periodically collected feces were used to assess the number of fecal pellets and fecal water content. Results : Polycan inhibited TNF-α-induced IL-8 expression in dose-dependent manner. Furthermore, Polycan suppressed TNF-α-induced phosphorylation of MAPKs (ERK1/2, p38 and JNK), degradation of Iκ-Bα and nuclear translocation of NF-κB. In an in vivo constipation model, the number of fecal pellets per food intake was significantly increased in rats administered with Polycan, both 1 day and 7 days after loperamide treatment. The water content of fecal pellets was restored in the Polycan groups starting 7 days after loperamide treatment. In addition, Polycan intake significantly enhanced the gastrointestinal transit ratio of a charcoal meal but reduced the number of intestinal fecal pellets. Conclusions : These results suggest that Polycan suppressed TNF-α-induced inflammation by blocking both the MAPK and NF-κB pathways in HT-29 cells. Additionally, in a loperamide-induced constipation model, Polycan showed clear laxative effects by increasing the number of fecal pellets, fecal water content, and intestinal transit ratio of a charcoal meal.

Keywords

Acknowledgement

이 연구는 산업통상자원부, 한국산업기술진흥원(KIAT)의 "바이오활성제제 글로벌 경쟁력강화사업 (과제번호:B0080207002074)의 지원을 받아 수행되었습니다.

References

  1. Park KY. Increased Health Functionality of Fermented Foods. Food Industry and Nutrition. 2012;17(1):1-8.
  2. Lee JJ, Lee YM, Jung SK, Kim KY, Lee MY. Effects of dietary mulberry leaf on loperamide-induced constipation in rats. Korean J Food Preserv. 2008;15(2):280-287.
  3. Kyung Bae Lee, A Ram Yu, In-Bong Song, Jeong Yong Park, and Min-Jung Bae. Role of Nutraceuticals in Gut Health. Food Industry and Nutrition. 2020;25(1):45-49.
  4. Mizoguchi E, Low D, Ezaki Y, Okada T. Recent updates on the basic mechanisms and pathogenesis of inflammatory bowel diseases in experimental animal models. Intest Res. 2020;18:151-167.
  5. Jang BI, Lee SH. Update in the pathogenesis of inflammatory bowel disease. J Korean Soc Coloproctol. 2008;24:302-309.
  6. Gilbert JA, Quinn RA, Debelius J, Xu ZZ, Morton J, Garg N, et al. Microbiome-wide association studies link dynamic microbial consortia to disease. Nature. 2016;535:94-103.
  7. Cumming JH. The effect of dietary fiber on fecal weight and composition. Spiller GA, editors. CRC Handbook of Dietary Fiber in Human Nutrition. Boca Raton: CRC Press. 1993;263-349.
  8. Xianli Wu and Alexander G. Schauss. Mitigation of Inflammation with Foods. J. Agric. Food Chem. 2012;60:6703-6717.
  9. Martin CR, Osadchiy V, Kalani A, Mayer EA. The brain-gut-microbiome axis. Cell Mol Gastroenterol Hepatol. 2018;6:133-148.
  10. Jeon HH, Park HJ. Diagnosis and treatment of constipation. Korean J Med. 2012;83(5):568-579.
  11. A. Olajide, O. A., Sarker, S. D. Anti-inflammatory natural products. Annual Reports in Medicinal Chemistry. 2020;55:153-177
  12. Chan GCF, Chan WK, Sze DMY. The effect of β-glucan on human immune and cancer cells. J Hematol Oncol. 2009;2:1-11.
  13. Song HS, Moon KY. In vitro antioxidant activity profiles of β-glucan isolated from yeast Saccharomayces cerevisiae and mutant Saccharomayces cerevisiae IS2. Food Sci. Biotechnol. 2006;15:437-440.
  14. Novak M, Vetvicka V. Beta-glucans, history, and the present: immunomodulatory aspects and mechanisms of action. J Immunotoxicol. 2008;5:47-57.
  15. Seo HP, Kim JM, Shin HD, Kim TK, Chang HJ, Park BR, Lee JW. Production of β-1,3/1,6-glucan by Aureobasidium pullulans SM-2001. Kor J Biotechnol Bioeng. 2002;17:376-380.
  16. Shin HD, Yang KJ, Park BR, Son CW, Jang HJ, Ku SK: Antiosteoporotic effect of polycan, beta-glucan from Aureobasidium, in ovariectomized osteoporotic mice. Nutrition. 2007; 23:853-860.
  17. Song HB, Park DC, Do GM, et al.: Effect of exopolymers of Aureobasidium pullulans on improving osteoporosis induced in ovariectomized mice. J Microbiol Biotechnol. 2006; 16:37-45.
  18. Chan GC, Chan WK, Sze DM. The effects of beta-glucan on human immune and cancer cells. J. Hematol. Oncol. 2009; 2:25
  19. Kim JH, Kim KR, Jin HJ, Im SU, Song KB, Choi YH. The Effect of Polycan-Calcium Gluconate Complex on Inflammatory Mediators from Periodontitis Patients. J Dent Hyg Sci. 2014;14(2):223-229.
  20. Do HJ, Kim Y-S, Oh TW. Effect of Polycan, a-Glucan from Aureobasidium pullulans SM-2001, on Inflammatory Response and Intestinal Barrier Function in DSS-Induced Ulcerative Colitis. Int. J. Mol. Sci. 2023;24:14773.
  21. Ko G-P, Unno T, Kim Y-S, Kim J. Dietary Polycan, a β-glucan originating from Aureobasidium pullulans SM-2001, attenuates high-fat-diet-induced intestinal barrier damage in obese mice by modulating gut microbiota dysbiosis. Food Sci Nutr. 2024; 00:1-12.
  22. Hwang SJ, Lim JM, Ku BH, Cheon DM, Jung YJ, Kim YS, et al. Effects of polysaccharide (polycan) derived from black yeast in dexamethasone-induced muscle atrophy cell model. Herbal Formula Science. 2021;29(1);45-55.
  23. Lee HJ. Study on the mechanism of consumer decision-making for purchasing health functional foods in the post-COVID era: focusing on latent class analysis (LCA). J. Korea Academia Industrial. 2023;24(9);610-622.
  24. Shimotoyodomo A, Meguro S, Hase T, Tokimitsu I, Sakata T. Decreased colonic mucus in rats with loperamide induced constipation. Comparative Biochemistry and Physiology - Part A: Mol and Integr Physiol. 2000;126(2):203-212.
  25. Kyanko MV, Canel RS, Ludemann V. et al. β -Glucan content and hydration properties of filamentous fungi. Appl Biochem Microbiol. 2013;49:41-45.
  26. Ahmad A, Kaleem M. β-Glucan as a Food Ingredient. In: Grumezescu AM, Holban AM. Biopolymers for Food Design. 1st ed. Amsterdam:Elsevier. 2018:351-381.
  27. Tejinder S, Bhupinder K, Harinder K. Flow behavior and functional properties of barley and oat water-soluble β-D-glucan rich extractions. Int. J. Food Prop. 2000;3:259-274.
  28. Kaur R, Sharma M, Ji D, Xu M, Agyei D. Structural Features, Modification, and Functionalities of Beta-Glucan. Fibers. 2020;8:1.