Herbal Formula Science (대한한의학방제학회지)

The Korean Medicine Society for the Herbal Formula Study (대한한의학방제학회)
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Analysis of Restraint Stress-induced Behavioral Alteration and Liver Injury in Mice

구속 스트레스로 인한 동물모델의 행동학적 변화와 간 손상

  • Ye Jin Yang (Departments of Veterinary Medicine, Gyeongsang National University) ;
  • Young Zoo You (Departments of Veterinary Medicine, Gyeongsang National University) ;
  • Min Jung Kim (Departments of Veterinary Medicine, Gyeongsang National University) ;
  • Jae Dong Son (Departments of Veterinary Medicine, Gyeongsang National University) ;
  • Tae Woo Oh (Korean Medicine (KM) Application Center, Korea Institute of Oriental Medicine) ;
  • Kwang Il Park (Departments of Veterinary Medicine, Gyeongsang National University)
  • 양예진 (경상국립대학교 수의과대학) ;
  • 유영주 (경상국립대학교 수의과대학) ;
  • 김민정 (경상국립대학교 수의과대학) ;
  • 손재동 (경상국립대학교 수의과대학) ;
  • 오태우 (한국한의학연구원 한의기술응용센터) ;
  • 박광일 (경상국립대학교 수의과대학)
  • Received : 2024.08.07
  • Accepted : 2024.08.20
  • Published : 2024.08.31
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Abstract

Background : Recent studies have shown that stress fundamentally influences the functional modulation of organ and stress-related disease causes high morbidity and mortality rates. Objective : The present research investigated the effect of restraint stress on psychological and physiological responses. Results : Body weight and food intake were changed in stress group. Body weight has continuously decreased, and food intake has been slightly altered. As a result of measuring each tissue's weight, the liver and kidney's weight loss was greater than that of other organs. The lipid profile of stressed animals showed significant increases in cholesterol (TC), triglyceride (TG), low-density lipoprotein (LDL), and high-density lipoprotein (HDL) levels compared to control. As hepatic marker enzymes, serum glutamic pyruvic transaminase (GPT; alanine aminotransferase), glutamic oxalacetic transaminase (GOT; aspartate aminotransferase), and lactic dehydrogenase (LDH) were increased in the stress group. However, levels of serum cortisol and corticosterone did not affect. Results of the behavioral tests show that the stress group has increased activity, sluggish movements, and anxiety in the central part compared with the control group through the open field test. In the forced swim test, the stress group models had a longer duration of slowing movement, and its rate also increased. Also, in immunoblotting, stress increased the inflammatory factors Inducible Nitric Oxide Synthase (iNOS), cyclooxygenase-2 (COX-2) and activated the mitogen-activated protein kinase (MAPK) pathway. Conclusions : We observed that mouse model were affected behavioral response and liver injury when exposed to restraint stress, indicating the importance of the restraint stress in the development of psychological and physiological processes.

Keywords

Acknowledgement

This research was funded by the Basic Science Research Program through the National Research Foundation of Korea (NRF) and supported by the Ministry of Education (NRF-2022R1I1A3053818), Republic of Korea.

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