Herbal Formula Science (대한한의학방제학회지)

The Korean Medicine Society for the Herbal Formula Study (대한한의학방제학회)
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LC-MS/MS analysis and antioxidant activity of Dendropanax morbiferus extract.

황칠나무(Dendropanax morbiferus) 잎 추출물의 LC-MS/MS 분석 및 항산화 효과

  • Min Jung Kim (College of Veterinary Medicine, Gyeongsang National University) ;
  • Jae Dong Son (College of Veterinary Medicine, Gyeongsang National University) ;
  • Ye Jin Yang (College of Veterinary Medicine, Gyeongsang National University) ;
  • Ji Woong Heo (College of Veterinary Medicine, Gyeongsang National University) ;
  • Hu Jang Lee (College of Veterinary Medicine, Gyeongsang National University) ;
  • Kwang Il Park (College of Veterinary Medicine, Gyeongsang National University)
  • 김민정 (경상국립대학교 수의과대학) ;
  • 손재동 (경상국립대학교 수의과대학) ;
  • 양예진 (경상국립대학교 수의과대학) ;
  • 허지웅 (경상국립대학교 수의과대학) ;
  • 이후장 (경상국립대학교 수의과대학) ;
  • 박광일 (경상국립대학교 수의과대학)
  • Received : 2024.08.05
  • Accepted : 2024.08.16
  • Published : 2024.08.31
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Abstract

Objective : The study's objective is to assess the components of Dendropanax morbifera (DM) utilizing UPLC-MS/MS and assess their antioxidant properties in order to establish fundamental information for quality control of herbal formulations. Methods : The DM leaves were ground into powder and extracted with water at 80℃. The extract was subsequently concentrated and subjected to freeze-drying for subsequent analysis. The LC-MS/MS analysis was performed using a 1260 series HPLC system and a 3200 QTrap tandem mass system in positive ion mode, with detection conducted at 280 nm. The Folin-Ciocalteu method was employed to measure the phenolic content, while a colorimetric method using aluminum chloride was used to determine the flavonoid content, with gallic acid and quercetin as standards, respectively. The evaluation of antioxidant activity was conducted through the measurement of DPPH radical scavenging activity, by adding the DPPH solution to the extract and recording the absorbance at 517 nm. Results : The UPLC-MS/MS analysis identified five polyphenolic compounds in the DM extract, specifically syringin, 6-hydroxyluteolin 7-O-laminaribioside, shaftoside, rutin, and kaempferol-3-O-rutinoside. The extract was found to contain a total phenolic content of 83.106 ± 0.21 mg GAE/g and a total flavonoid content of 87.963 ± 1.014 mg QE/g. The DM extract demonstrated substantial antioxidant properties, resulting in a reduction of DPPH radicals that was evident at concentrations as low as 40 ㎍/㎖. Conclusions : The study determined important polyphenolic compounds in DM and established its considerable antioxidant efficacy. These findings provide evidence for the efficacy of DM in disease prevention related to oxidative stress and establish a foundation for ensuring quality control in herbal preparations.

Keywords

Acknowledgement

This research was funded by the Basic Science Research Program through the National Research Foundation of Korea (NRF) and supported by the Ministry of Education (NRF-2022R1I1A3053818), Republic of Korea.

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