Herbal Formula Science (대한한의학방제학회지)

The Korean Medicine Society for the Herbal Formula Study (대한한의학방제학회)
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Antioxidant Potential of Cinnamomum cassia Ethanolic Extract: Identification Of Compounds

계피 에탄올 추출물의 유효성분 분석 및 항산화 효능 평가

  • Ji Woong Heo (Departments of Veterinary Medicine, Gyeongsang National University) ;
  • Jae Dong Son (Departments of Veterinary Medicine, Gyeongsang National University) ;
  • Ye Jin Yang (Departments of Veterinary Medicine, Gyeongsang National University) ;
  • Min Jung Kim (Departments of Veterinary Medicine, Gyeongsang National University) ;
  • Ju Hye Yang (Korean Medicine (KM) Application Center, Korea Institute of Oriental Medicine) ;
  • Kwang Il Park (Departments of Veterinary Medicine, Gyeongsang National University)
  • 허지웅 (경상국립대학교 수의과대학) ;
  • 손재동 (경상국립대학교 수의과대학) ;
  • 양예진 (경상국립대학교 수의과대학) ;
  • 김민정 (경상국립대학교 수의과대학) ;
  • 양주혜 (한국한의학연구원 한의기술응용센터) ;
  • 박광일 (경상국립대학교 수의과대학)
  • Received : 2024.07.09
  • Accepted : 2024.08.11
  • Published : 2024.08.31
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Abstract

Objectives : Natural products containing bioactive compounds with high antioxidant activity are potentially important sources that can contribute to the improvement of various diseases. Therefore, the aim of this study was to investigate phenolic compounds of Cinnamomum cassia (C. cassia) ethanolic extract (CCEE). And then we evaluated the antioxidant effect. Methods : We used liquid chromatography with tandem mass spectrometry (LC-MS/MS) to identify the compounds in CCEE. LC-MS/MS was performed in positive ion mode using Shimadzu, Nexera HPLC system and IDA TOF mass system. Solvent A was distilled water and solvent B was acetonitrile as mobile phase. The analysis was performed at a flow rate of 0.5 ml/min, column temperature of 35 ℃ and wavelength of 284 nm. The antioxidant effect of CCEE was analyzed using DPPH (2,2-diphenyl-2-picrylhydrazyl free radical) and ABTS (2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid)). In addition, total phenolics and total flavonoids contents were measured to determine antioxidant effects. Results : Analysis using LC-MS/MS identified four compounds: Coumarin, Trans-cinnamaldehyde, Trans-cinnamic acid, and 2-Methoxycinnamaldehyde. Free radicals decreased in a concentration-dependent manner starting from 10 ㎍/ml of CCEE, and decreased to a level similar to Ascorbic acid (AA) from a concentration of 60 ㎍/ml onwards. Conclusions : Based on the findings, CCEE exhibits strong antioxidant activity as evidenced by the presence of Coumarin, Trans-cinnamaldehyde, Trans-cinnamic acid, and 2-Methoxycinnamaldehyde. Consequently, this study suggests that CCEE can serve as an important source of natural antioxidants and can be efficiently used in the management of oxidative stress diseases.

Keywords

Acknowledgement

This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (NRF-2022R1I1A3053818).

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