Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Biodegradation of Low-Density Polyethylene by Acinetobacter guillouiae PL211 Isolated from the Waste Treatment Facility

  • Ye-Jin Kim (Department of Bioengineering and Technology, Kangwon National University) ;
  • Jang-Sub Lee (Department of Bioengineering and Technology, Kangwon National University) ;
  • Jeong-Ann Park (Department of Environmental Engineering, Kangwon National University) ;
  • Hyun-Ouk Kim (Department of Bioengineering and Technology, Kangwon National University) ;
  • Kwang Suk Lim (Department of Bioengineering and Technology, Kangwon National University) ;
  • Suk-Jin Ha (Department of Bioengineering and Technology, Kangwon National University)
  • Received : 2024.04.04
  • Accepted : 2024.06.07
  • Published : 2024.06.28
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Abstract

Plastics are consistently produced owing to their practicality and convenience. Unmanaged plastics enter the oceans, where they adversely impact marine life, and their degradation into nano-plastics due to sunlight and weathering is of concern for all living beings. Nano-plastics affect humans via the food chain, emphasizing the necessity for effective solutions. Microbial biodegradation has been suggested as a solution, offering the advantages of minimal environmental impact and the utilization of decomposition byproducts in microbial metabolic pathways. In this study, fifty-seven bacterial strains were isolated and identified from a waste-treatment facility. Cultivation in a minimum medium with low-density polyethylene (LDPE) beads as the sole carbon source resulted in the selection of the LDPE-degrading strain Acinetobacter guillouiae PL211. The selected strain was cultured at high cell density with LDPE as a carbon source, and Fourier transform infrared (FT-IR) analysis confirmed chemical changes on the LDPE bead's surface. Field-emission scanning electron microscopy (FE-SEM) analysis revealed substantial biodegradation of the LDPE surface. These results demonstrated the capability of A. guillouiae PL211 to biodegrade LDPE beads. This discovery demonstrates the potential of an environmentally friendly process to addressing polyethylene waste issues.

Keywords

Acknowledgement

This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF).

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