Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Uncovering the Antibacterial Potential of a Peptide-Rich Extract of Edible Bird's Nest against Staphylococcus aureus

  • Thi-Phuong Nguyen (NTT Hi-Tech Institute, Nguyen Tat Thanh University) ;
  • Tang Van Duong (Vietnam National Museum of Nature, Vietnam Academy of Science and Technology) ;
  • Thai Quang Le (NTT Hi-Tech Institute, Nguyen Tat Thanh University) ;
  • Khoa Thi Nguyen (NTT Hi-Tech Institute, Nguyen Tat Thanh University)
  • Received : 2024.02.29
  • Accepted : 2024.06.25
  • Published : 2024.08.28
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Abstract

The diverse pharmacological properties of edible bird's nest (EBN) have been elucidated in recent years; however, investigations into its antibacterial effects are still limited. In the present study, we explored the antibacterial activity of a peptide-rich extract of EBN against Staphylococcus aureus, a notorious pathogen. The EBN extract (EEE) was prepared by soaking EBN in 80% ethanol for 2 days at 60℃. Biochemical analyses showed that peptides at the molecular weight range of 1.7-10 kDa were the major biochemical compounds in the EEE. The extract exhibited strong inhibition against S. aureus at a minimum inhibitory concentration (MIC) of 125 ㎍/ml and a minimum bactericidal concentration (MBC) of 250 ㎍/ml. This activity could be attributed to the impact of the extract on cell membrane integrity and potential, biofilm formation, and reactive oxidative species (ROS) production. Notably, the expression of biofilm- and ROS-associated genes, including intercellular adhesion A (icaA), icaB, icaC, icaD, and superoxide dismutase A (sodA), were deregulated in S. aureus upon the extract treatment. Our findings indicate a noteworthy pharmacological activity of EBN that could have potential application in the control of S. aureus.

Keywords

Acknowledgement

We are grateful to Phuoc Loc Thi Nguyen, Director of Phuoc Tin Development Trading Service Company, Ltd. for kindly providing edible bird's nest. This research did not receive any specific grant from funding agencies in the public, commercial, or not-for-profit sectors.

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