Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Bacillus siamensis 3BS12-4 Extracellular Compounds as a Potential Biological Control Agent against Aspergillus flavus

  • Patapee Aphaiso (Graduate School, Khon Kaen University) ;
  • Polson Mahakhan (Department of Microbiology, Faculty of Science, Khon Kaen University) ;
  • Jutaporn Sawaengkaew (Department of Microbiology, Faculty of Science, Khon Kaen University)
  • Received : 2024.02.29
  • Accepted : 2024.06.18
  • Published : 2024.08.28
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Abstract

Aspergillus flavus, the primary mold that causes food spoilage, poses significant health and economic problems worldwide. Eliminating A. flavus growth is essential to ensure the safety of agricultural products, and extracellular compounds (ECCs) produced by Bacillus spp. have been demonstrated to inhibit the growth of this pathogen. In this study, we aimed to identify microorganisms efficient at inhibiting A. flavus growth and degrading aflatoxin B1. We isolated microorganisms from soil samples using a culture medium containing coumarin (CM medium) as the sole carbon source. Of the 498 isolates grown on CM medium, only 132 bacterial strains were capable of inhibiting A. flavus growth. Isolate 3BS12-4, identified as Bacillus siamensis, exhibited the highest antifungal activity with an inhibition ratio of 43.10%, and was therefore selected for further studies. The inhibition of A. flavus by isolate 3BS12-4 was predominantly attributed to ECCs, with a minimum inhibitory concentration and minimum fungicidal concentration of 0.512 g/ml. SEM analysis revealed that the ECCs disrupted the mycelium of A. flavus. The hydrolytic enzyme activity of the ECCs was assessed by protease, β-1,3-glucanase, and chitinase activity. Our results demonstrate a remarkable 96.11% aflatoxin B1 degradation mediated by ECCs produced by isolate 3BS12-4. Furthermore, treatment with these compounds resulted in a significant 97.93% inhibition of A. flavus growth on peanut seeds. These findings collectively present B. siamensis 3BS12-4 as a promising tool for developing environmentally friendly products to manage aflatoxin-producing fungi and contribute to the enhancement of agricultural product safety and food security.

Keywords

Acknowledgement

This research was co-financed by the research capability enhancement program through graduate student scholarship, Faculty of Science, Khon Kaen University; and the framework of Plant Genetic Project under The Royal Initiative of Her Royal Highness Princess Maha Chakri Sirindhorn, Khon Kaen University, which is supported by the Fundamental Fund of Khon Kaen University and the National Science, Research and Innovation Fund (NSRF), Thailand.

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