The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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First Report of Anthracnose Caused by Colletotrichum sojae on Peanut in Korea

Colletotrichum sojae에 의한 땅콩 탄저병 발생 보고

  • Shinhwa Kim (National Institute of Crop Science, Rural Development Administration) ;
  • Soo Yeon Choi (National Institute of Crop Science, Rural Development Administration) ;
  • Hyunjung Chung (National Institute of Crop Science, Rural Development Administration) ;
  • Nak Jung Choi (National Institute of Crop Science, Rural Development Administration) ;
  • Bo Yoon Seo (National Institute of Crop Science, Rural Development Administration) ;
  • Sang-Min Kim (National Institute of Crop Science, Rural Development Administration)
  • 김신화 (농촌진흥청 국립식량과학원 작물기초기반과) ;
  • 최수연 (농촌진흥청 국립식량과학원 작물기초기반과) ;
  • 정현정 (농촌진흥청 국립식량과학원 작물기초기반과) ;
  • 최낙중 (농촌진흥청 국립식량과학원 작물기초기반과) ;
  • 서보윤 (농촌진흥청 국립식량과학원 작물기초기반과) ;
  • 김상민 (농촌진흥청 국립식량과학원 작물기초기반과)
  • Received : 2023.12.14
  • Accepted : 2024.03.29
  • Published : 2024.03.31
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Abstract

In August 2023, leaf spot disease was observed in peanuts in Cheongju-si, Korea. Leaf spots occurred at the leaf margins and the lesions gradually expanded. Diseased leaf areas were light or dark brown and irregular in shape. A fungal isolate was obtained from symptomatic leaf and cultured on potato dextrose agar (PDA) medium at 25℃. An isolate was identified as Colletotrichum sojae based on morphological characteristics and sequences of the internal transcribed spacers, glyceraldehyde-3-phosphate dehydrogenase, chitin synthase-1, actin, and 𝛽-tubulin genes. Pathogenicity tests were performed on peanut seedlings in a conidial suspension (1×106 conidia/mL). Lesions were observed on the peanut leaf 5 d after inoculation. The pathogen was re-isolated from the lesions of the inoculated leaves. To the best of our knowledge, this is the first report of anthracnose on peanut caused by C. sojae in Korea.

2023년 8월 청주 땅콩 재배포장에서 기보고되어 있는 점무늬 병징과는 다른 양상의 잎 반점을 발견하였다. 갈색 반점은 주로 잎 가장자리에 형성되었으며, 반점 부위에서 원인균을 분리하였다. 순수 분리한 병원균을 땅콩 유묘에 인공접종하여 병원성 검정을 수행하였다. 그 결과, 접종 5-7일 뒤에 유사한 병징을 확인할 수 있었고 병징 부위에서 동일한 균을 재분리하였다. 분리한 균주의ACT-CHS-1-GAPDH-ITS-TUB2 영역을 이용하여 동정 및 유연관계 분석을 수행한 결과 C. sojae임을 확인하였다. 따라서 본 연구는 국내 최초로C. sojae에 의해 발생한 땅콩 탄저병을 보고하고자 한다.

Keywords

Acknowledgement

This study was supported by grant (Project No. PJ016095012023) funded by the Rural Development Administration.

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