The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Correlation Analysis Study Between Spent Mushroom Substrate and Microbial Community

수확 후 버섯 배지와 미생물 군집의 상관관계 분석 연구

  • In Kyu Lee (Department of Applied Plant Sciences, Interdisciplinary Program in Smart Agriculture, Kangwon National University) ;
  • Hyun Seung Kim (Department of Applied Plant Sciences, Interdisciplinary Program in Smart Agriculture, Kangwon National University) ;
  • Ji Min Woo (Department of Applied Plant Sciences, Kangwon National University) ;
  • Won Jun Chang (Department of Applied Plant Sciences, Interdisciplinary Program in Smart Agriculture, Kangwon National University) ;
  • Eun Jeong Byeon (Department of Applied Plant Sciences, Kangwon National University) ;
  • Ki Byung Park (Jungbubiotech) ;
  • Youn Su Lee (Department of Applied Plant Sciences, Interdisciplinary Program in Smart Agriculture, Kangwon National University)
  • 이인규 (강원대학교 스마트농업융합학과) ;
  • 김현승 (강원대학교 스마트농업융합학과) ;
  • 우지민 (강원대학교 식물자원응용과학과) ;
  • 장원준 (강원대학교 스마트농업융합학과) ;
  • 변은정 (강원대학교 식물자원응용과학과) ;
  • 박기병 (중부바이오텍) ;
  • 이윤수 (강원대학교 스마트농업융합학과)
  • Received : 2024.01.31
  • Accepted : 2024.03.29
  • Published : 2024.03.31
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Abstract

Next-generation sequencing of shiitake, oyster, and king oyster spent mushroom substrates collected from Chun-cheon, Yeo-ju, Hong-cheon, Gwang-ju, Ui-ryeong, and A-san was performed. Metabarcoding analysis using amplicon sequence variants was performed to confirm the microbial content ratio in the medium after harvesting the collected mushrooms; the ratio of the contents of various microorganisms in the medium after mushroom harvest varied depending on the materials added to produce the mushroom medium. The WPGMA analysis of the similarity between microbial communities, which was based on the β-diversity, confirmed that the microbial communities in the substrates of the different mushroom varieties were similar.

버섯 배지에 첨가되는 재료에 따라 변하는 수확 후 배지내의 세균 군집도를 확인하여 더 넓은 재활용 연구에 기여하고자 춘천, 여주, 홍천, 광주, 의령, 아산에서 수집한 표고, 느타리, 새송이버섯의 수확 후 배지를 대상으로 차세대 염기서열 분석을 진행하였다. ASV 값을 기반으로 α-diversity인 Rarefaction, Chao1, Shannon, Gini-Simpson를 분석한 결과, 유일하게 폐면이 혼합되어 있는 느타리버섯 수확 후 배지인 H-NT 처리구의 세균다양성이 매우 풍부하였다. 또한 β-diversity의 WPGMA를 이용하여 처리구간 세균 군집의 유사성을 분석한 결과, 버섯 종류에 따라 유연관계가 가까운 것을 확인하였다. 본 연구를 시작으로 작물 및 토양에 유용한 특정 세균 비율이 높게 분포하고 있는 수확 후 배지를 연구한다면 맞춤형 유기농자재로서의 활용 가능성이 있음을 시사한다.

Keywords

Acknowledgement

This study was supported by Industry-academia-research collaboration R&D project (RS-2023-00223480) from the Ministry of SMEs and Startups.

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