Journal of Periodontal and Implant Science

Korean Academy of Periodontology (대한치주과학회)
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Tissue integration patterns of non-crosslinked and crosslinked collagen membranes: an experimental in vivo study

  • Xiang Jin (Department of Periodontology, Research Institute of Periodontal Regeneration, Yonsei University College of Dentistry) ;
  • Jin-Young Park (Department of Periodontology, Research Institute of Periodontal Regeneration, Yonsei University College of Dentistry) ;
  • Jung-Seok Lee (Department of Periodontology, Research Institute of Periodontal Regeneration, Yonsei University College of Dentistry) ;
  • Ui-Won Jung (Department of Periodontology, Research Institute of Periodontal Regeneration, Yonsei University College of Dentistry) ;
  • Seong-Ho Choi (Department of Periodontology, Research Institute of Periodontal Regeneration, Yonsei University College of Dentistry) ;
  • Jae-Kook Cha (Department of Periodontology, Research Institute of Periodontal Regeneration, Yonsei University College of Dentistry)
  • Received : 2022.07.15
  • Accepted : 2022.10.12
  • Published : 2023.06.30
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Abstract

Purpose: Non-crosslinked and crosslinked collagen membranes are known to exhibit distinct degradation characteristics, resulting in contrasting orientations of the adjacent tissues and different biological processes. The aim of this study was to conduct a histomorphometric assessment of non-crosslinked and crosslinked collagen membranes regarding neovascularization, tissue integration, tissue encapsulation, and biodegradation. Methods: Guided bone regeneration was performed using either a non-crosslinked (BG) or a crosslinked collagen membrane (CM) in 15 beagle dogs, which were euthanized at 4, 8, and 16 weeks (n=5 each) for histomorphometric analysis. The samples were assessed regarding neovascularization, tissue integration, encapsulation, the remaining membrane area, and pseudoperiosteum formation. The BG and CM groups were compared at different time periods using nonparametric statistical methods. Results: The remaining membrane area of CM was significantly greater than that of BG at 16 weeks; however, there were no significant differences at 4 and 8 weeks. Conversely, the neovascularization score for CM was significantly less than that for BG at 16 weeks. BG exhibited significantly greater tissue integration and encapsulation scores than CM at all time periods, apart from encapsulation at 16 weeks. Pseudoperiosteum formation was observed in the BG group at 16 weeks. Conclusions: Although BG membranes were more rapidly biodegraded than CM membranes, they were gradually replaced by connective tissue with complete integration and maturation of the surrounding tissues to form dense periosteum-like connective tissue. Further studies need to be performed to validate the barrier effect of the pseudoperiosteum.

Keywords

Acknowledgement

This work was supported by the Korea Medical Device Development Fund grant funded by the Korean government (the Ministry of Science and ICT, the Ministry of Trade, Industry and Energy, the Ministry of Health & Welfare, the Ministry of Food and Drug Safety) (Project Number: KMDF_PR_20200901_0240), and also by a grant of the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), funded by the Ministry of Health & Welfare, Republic of Korea (grant number: HI20C2114).

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