Journal of Periodontal and Implant Science

Korean Academy of Periodontology (대한치주과학회)
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Sinus augmentation with poly(ε) caprolactone-β tricalcium phosphate scaffolds, mesenchymal stem cells and platelet rich plasma for one-stage dental implantation in minipigs

  • Jeong-Hun Nam (Department of Oral & Maxillofacial Surgery, School of Dentistry, Seoul National University) ;
  • Akram Abdo Almansoori (Department of Oral & Maxillofacial Surgery, School of Dentistry, Seoul National University) ;
  • Oh-Jun Kwon (Department of Oral & Maxillofacial Surgery, School of Dentistry, Seoul National University) ;
  • Young-Kwon Seo (Department of Chemical and Biochemical Engineering, College of Engineering, Dongkuk University) ;
  • Bongju Kim (Dental Life Science Research Institute, Innovation Research & Support Center for Dental Science, Seoul National University Dental Hospital) ;
  • Young-Kyun Kim (Department of Oral & Maxillofacial Surgery, Section of Dentistry, Seoul National University Bundang Hospital) ;
  • Jong-Ho Lee (Dental Life Science Research Institute, Innovation Research & Support Center for Dental Science, Seoul National University Dental Hospital) ;
  • KangMi Pang (Department of Oral & Maxillofacial Surgery, Seoul National University Gwanak Dental Hospital)
  • Received : 2023.01.13
  • Accepted : 2023.04.03
  • Published : 2023.06.30
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Abstract

Purpose: This study evaluated the efficacy of a tube-shaped poly(ε) caprolactone - β tricalcium phosphate (PCL-TCP) scaffold with the incorporation of human umbilical cord-derived mesenchymal stem cells (hUCMSCs) and platelet-rich plasma (PRP) for bone regeneration in the procedure of single-stage sinus augmentation and dental implantation in minipigs. Methods: Implants were placed in the bilateral sides of the maxillary sinuses of 5 minipigs and allocated to a PCL-TCP+hUCMSCs+PRP group (n=5), a PCL-TCP+PRP group (n=5), and a PCL-TCP-only group (n=6). After 12 weeks, bone regeneration was evaluated with soft X-rays, micro-computed tomography, fluorescence microscopy, and histomorphometric analysis. Results: Four implants failed (2 each in the PCL-TCP+hUCMSCs+PRP and PCLTCP+hUCMSC groups). An analysis of the grayscale levels and bone-implant contact ratio showed significantly higher mean values in the PCL-TCP+hUCMSCs+PRP than in the PCL-TCP group (P=0.045 and P=0.016, respectively). In fluoromicroscopic images, new bone formation around the outer surfaces of the scaffolds was observed in the PCLTCP+hUCMSCs+PRP group, suggesting a tenting effect of the specially designed scaffolds. Bone regeneration at the scaffold-implant interfaces was observed in all 3 groups. Conclusions: Using a tube-shaped, honeycombed PCL-TCP scaffold with hUCMSCs and PRP may serve to enhance bone formation and dental implants' osseointegration in the procedure of simultaneous sinus lifting and dental implantation.

Keywords

Acknowledgement

This research was supported by a grant from the Korea Health Technology R&D Project through the Korea Health Industry Development Institute and funded by the Ministry of Health and Welfare, Republic of Korea (HI20C2114).

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