Journal of Plant Biotechnology

  • 제50권
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  • Pages.19-26
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  • 2023
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  • 1229-2818(pISSN)
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  • 2384-1397(eISSN)
한국식물생명공학회 (The Korean Society of Plant Biotechnology)
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인삼 약 배양을 통한 배 발생과 식물 재분화 및 유세포 분석기를 이용한 배수성 검정

Embryogenesis and plant regeneration of Panax ginseng Meyer via anther culture and ploidy assessment using flow cytometry

  • 이정우 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 방경환 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 김동휘 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 김장욱 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 김영창 (농촌진흥청 연구정책국 융복합혁신전략팀) ;
  • 조익현 (단국대학교 생명공학대학 생명자원학부)
  • Jung-Woo Lee (Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, Rural Development Administration) ;
  • Kyong-Hwan Bang (Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, Rural Development Administration) ;
  • Dong-Hwi Kim (Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, Rural Development Administration) ;
  • Jang-Uk Kim (Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, Rural Development Administration) ;
  • Young-Chang Kim (Research Policy Bureau, Rural Development Administration) ;
  • Ick-Hyun Jo (Department of Crop Science and Biotechnology, Dankook University)
  • 투고 : 2023.02.20
  • 심사 : 2023.03.21
  • 발행 : 2023.03.29
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초록

이번 연구에서 인삼의 약 배양을 통한 배 형성과 식물체의 재분화가 확인되었다. 인삼의 약 배양에서 캘러스 유도에 적합한 기본 배지는 MS 배지였고 sucrose 농도는 3%였으며, 배 형성에 적합한 호르몬 농도는 2,4-D 1.0 mg/L 또는 3.0 mg/L이었다. 저온 전처리에 의한 인삼 약 배양의 캘러스 유도와 배 형성의 효율성 차이는 확인할 수 없었다. 인삼의 품종별 약 배양의 효율성을 검정한 결과, 청선 품종의 배 형성률이 가장 우수하였으며 선운 품종은 배 형성이 관찰되지 않았다. 약 배양으로 유도된 캘러스의 배수성을 검정한 결과, 반수체 캘러스가 확인되었지만 이 캘러스에서 배는 형성되지 않았다. 약 배양 유래의 배를 발아배지에 치상하였을 때 정상적으로 식물체가 재분화 하였으며, 이 식물체의 배수성을 검정한 결과, 모두 2배체로 확인되었다. 추후 DNA 마커를 이용한 약 배양 유래 식물체의 동형접합성의 확인이 필요하다고 판단된다. 인삼 약 배양을 통하여 동형접합성 식물체를 생산할 수 있다면 인삼 육종 효율성을 증진시키는데 크게 기여할 수 있을 것이다.

Korean ginseng (Panax ginseng Meyer) is an economically important plant because of it is rich in saponins. It is mainly cultivated in Asia, including Korea and China. Since ginseng requires a long breeding period due to juvenility, homozygote production techniques, such as anther culture, must be urgently established. In the present study, callus induction and embryogenesis through anther culture were observed in P. ginseng. Murashige and Skoog medium was used as the basal medium suitable for callus induction. When the medium was supplemented with 3% sucrose, the callus induction rate was high and the callus size was large. Cold pretreatment did not significantly affect callus induction and embryogenesis. Embryogenesis was the most efficient when the embryo-formation medium was supplemented with 1.0 or 3.0 mg/L 2,4-dichlorophenoxyacetic acid. Cultivar significantly affected anther culture efficiency. Specifically, 'Cheongseon' showed the highest embryo-formation efficiency, whereas no embryogenesis occurred in 'Sunun'. Ploidy assessment revealed the haploid status of the induced calli. Embryos derived from anther culture formed shoots upon transfer to germination medium, although no difference in ploidy was noted between the induced callus and control. Overall, the anther culture conditions established in the present study may contribute to the production of homozygous P. ginseng plants in the future.

키워드

과제정보

본 연구는 농촌진흥청에서 주관하는 인삼 육종효율 증진을 위한 기반기술 개발(과제번호: PJ01018702)의 연구비 지원에 의해 이루어진 결과로 이에 감사드립니다.

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