Journal of Applied Biological Chemistry

  • Volume 66
  • /
  • Pages.7-14
  • /
  • 2023
  • /
  • 1976-0442(pISSN)
  • /
  • 2234-7941(eISSN)
The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Effects of Fermented Achyranthes japonica Nakai, Angelica gigas Nakai, and Eucommia ulmoides Oliver Extracts on Regulation of Apoptosis in Articular Chondrocytes

Primary Chondrocytes에서 발효우슬, 당귀, 두충 복합물의 세포사멸 조절 효과

  • Dakyung Kim (Department of Medical Nutrition, Kyung Hee University) ;
  • Wonhee Jo (Department of Medical Nutrition, Kyung Hee University) ;
  • Minhee Lee (Department of Medical Nutrition, Kyung Hee University) ;
  • Hyun Cheol Jeong (Biohealthcare R&D Center, Hyundaibioland Co., Ltd.) ;
  • Sung-Jin Lee (Biohealthcare R&D Center, Hyundaibioland Co., Ltd.) ;
  • Seunghun Lee (Biohealthcare R&D Center, Hyundaibioland Co., Ltd.) ;
  • Jeongmin Lee (Department of Medical Nutrition, Kyung Hee University)
  • Received : 2022.12.09
  • Accepted : 2023.01.06
  • Published : 2023.12.31
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Abstract

The effects of fermented Achyranthes japonica Nakai, Angelica gigas Nakai, and Eucommia ulmoides Oliver extracts (FAAE) on regulation of inflammation and apoptosis were investigated in primary cultured rat cartilage cells. To identify the protective effects of FAAE against H2O2, cell survival was measured by MTT assay. Smad3, Collagen type I, MMP3, and MMP13 were measured by real-timpe PCR and westernbot and the inflammatory (NF-κB pathway, COX-2, iNOS) factors were determined by western blot. The apoptosis related factors (JNK, c-Fos, c-Jun, caspase 3, Bax, and Bcl-2) were determined by western blot. FAAE significantly increased the follwing: H2O2 treated cell survival, mRNA and protein expression of Smad 3, collagen type I. In addition, FAAE significantly decreased the protein expression of inflammatory and apoptosis related factors. This study suggests that FAAE have a protection effect of chondrocytes through inhibition of inflammation and apoptosis. Thus, FAAE is a therapeutic potential food componet in osteoarthritis.

SD rat에서 primary culture한 chondrocyte에서 발효우슬, 당귀, 두충 추출 복합물(FAAE)이 염증 및 세포사멸 조절에 미치는 영향을 알아보고자 하였다. FAAE는 H2O2에 대한 세포 생존률, Smad3, Collagen type 1의 mRNA 및 단백질 발현을 증가시켰고, 염증 및 세포사멸 관련인자(NF-κB pathway, COX-2, iNOS, JNK, c-Fos, c-Jun, caspase 3, Bax, Bcl-2)의 단백질 발현을 감소시켰다. 본 연구는 FAAE가 염증 및 세포 사멸 억제를 통해 연골세포 보호효과가 있음을 시사한다.

Keywords

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