Anatomy and Cell Biology

  • 제55권3호
  • /
  • Pages.320-329
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  • 2022
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  • 2093-3665(pISSN)
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  • 2093-3673(eISSN)
대한해부학회 (Korean Association of Anatomists)
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Tamarindus indica ameliorates behavioral and cytoarchitectural changes in the cerebellar cortex following prenatal aluminum chloride exposure in Wistar rats

  • Ibe Michael Usman (Department of Human Anatomy, Faculty of Biomedical Sciences, Kampala International University) ;
  • Samuel Sunday Adebisi (Department of Human Anatomy, College of Medicine and Health Science, Ahmadu Bello University) ;
  • Sunday Abraham Musa (Department of Human Anatomy, College of Medicine and Health Science, Ahmadu Bello University) ;
  • Ibrahim Abdullahi Iliya (Department of Human Anatomy, Federal University Dutse) ;
  • Victor Bassey Archibong (Department of Human Anatomy, Faculty of Biomedical Sciences, Kampala International University) ;
  • Ann Monima Lemuel (Department of Human Anatomy, Faculty of Biomedical Sciences, Kampala International University) ;
  • Keneth Iceland Kasozi (Infection Medicine, Deanery of Biomedical Sciences, College of Medicine and Veterinary Medicine, The University of Edinburgh)
  • 투고 : 2022.02.14
  • 심사 : 2022.04.17
  • 발행 : 2022.09.30
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초록

Aluminium exposure has been linked with developmental neurotoxicity in humans and experimental animals. The study aimed to evaluate the ameliorative effect of Tamarindus indica on the developing cerebellar cortex, neurobehavior, and immunohistochemistry of the cerebellar cortex following prenatal aluminum chloride (AlCl3) exposure. Pregnant timed Wistar rats were divided into 5 groups (n=4). Group I (negative control) was given distilled water, group II was treated with 200 mg/kg of AlCl3, group III were given 200 mg/kg of AlCl3 and 400 mg/kg of ethyl acetate leaf fraction of Tamarindus indica (EATI), group IV were given 200 mg/kg of AlCl3 and 800 mg/kg of EATI, and group V were treated with 200 mg/kg of AlCl3 s/c and 300 mg/kg of vitamin E for 14 days (prenatal day 7-21) via the oral route. Male pups (n=6) were randomly selected and taken for neurobehavioral studies, and humanely sacrificed via intraperitoneal injection of thiopental sodium. The cerebellum was removed, fixed and tissue processed for histological and immunohistochemical studies. The results revealed that prenatal AlCl3 exposure impacted neurodevelopment and neurobehaviour among exposed pups. Prenatal AlCl3 exposure was marked with delayed cytoarchitectural development of the cerebellar cortex and increased GFAP expression in the cerebellar cortex. On the other hand, treatment with EATI and vitamin E were marked with significant improvements. The present study therefore concluded treatment with EATI shows an ameliorative effect to prenatal AlCl3 exposure.

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과제정보

The researchers acknowledge the support of the Department of Human Anatomy, Ahmadu Bello University, Zaria, for providing suitable working conditions for the present research.

참고문헌

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