Anatomy and Cell Biology

  • 제55권3호
  • /
  • Pages.311-319
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  • 2022
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  • 2093-3665(pISSN)
  • /
  • 2093-3673(eISSN)
대한해부학회 (Korean Association of Anatomists)
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Effect of caffeine on genes expressions of developing retinas in the chick model

  • Thanyarat Lekchaoum (Department of Anatomy, Faculty of Science, Mahidol University) ;
  • Aticha Buddawong (Chulabhorn International College of Medicine, Thammasat University) ;
  • Sunalin Ahi (Department of Anatomy, Faculty of Science, Mahidol University) ;
  • Nichapha Chandee (Department of Anatomy, Faculty of Science, Mahidol University) ;
  • Wattana Weerachatyanukul (Department of Anatomy, Faculty of Science, Mahidol University) ;
  • Somluk Asuvapongpatana (Department of Anatomy, Faculty of Science, Mahidol University)
  • 투고 : 2022.02.14
  • 심사 : 2022.04.17
  • 발행 : 2022.09.30
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초록

It has been reported that overconsumption of caffeine during pregnancy leads to a deleterious effect within the nervous tissues during embryonic development. In this study, we further extrapolated the effect of caffeine in the developing retinas, which is known to be one of the most sensitive tissues in chick embryos. Morphological changes of retinal thickness and organization of neuroretinal epithelium were monitored using three gene markers, Atoh7, FoxN4, and Lim1. Upon treating with a single dose of caffeine (15 µmol at embryonic day 1 [E1]), relative thicknesses of developing retinas (particularly of E7 and E9) were significantly altered. Among the three genes studied, the expression pattern of Atoh7 was notably altered while those of FoxN4, and Lim1 mRNA showed only a slight change in these developing retinas. Quantitative polymerase chain reaction results supported the most notable changes of Atoh7 but not FoxN4, and Lim1 gene in the developing retinas, particularly at E7. The effect of caffeine towards other organs during development should be extrapolated and the awareness of its intensive consumption should be raised.

키워드

과제정보

This study was supported by a grant from the Development and Promotion of Science and Technology Talents Project (DPST), Thailand. We also thank the Center of Nanoimaging (CNI) and the Central Instrument Facility (CIF), Faculty of Science, Mahidol University, for providing instrumental support throughout this work.

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