Korean Journal of Plant Resources (한국자원식물학회지)

The Plant Resources Society of Korea (한국자원식물학회)
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Plantlet Regeneration and PLBs Propagation of Bulbophyllum auricomum Lindl.

  • Aung, Win Theingi (Department of Lifestyle Medicine, Jeonbuk National University) ;
  • Lian, Thang Tung (Useful Plant Resource Center. Dagon University, Dagon Myothit Township (East)) ;
  • Aung, Zaw Phyo (Useful Plant Resource Center. Dagon University, Dagon Myothit Township (East)) ;
  • Bang, Keuk Soo (Department of Lifestyle Medicine, Jeonbuk National University) ;
  • Baek, Seung Hwa (Department of Biofood Science and Biotechnology / Department of Culinary and Baking, Chungbuk Provincial University)
  • Received : 2022.05.11
  • Accepted : 2022.06.08
  • Published : 2022.08.01
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Abstract

Bulbophyllum auricomum Lindl. is very popular among orchid growers due to the attractive fragrance of its flowers and has become an endangered orchid in Myanmar. In this study, we carried out an aseptic technique that can be used to conserve this endangered orchid species. The seeds of B. auricomum Lindl. were obtained from artificial pollination and cultured in MS basal medium for seed germination. The effect of coconut water and BAP in MS basal medium on callus induction was investigated. The highest callus induction was found at 2.0 mg/L BAP. The maximum growth of protocorm-like bodies (PLBs) was evaluated, and the best response was observed on MS medium supplemented with 150 mL/L coconut water at pH 5.6. MS basal medium supplemented with 150 mL/L coconut water along with 2.0 mg/L BAP and 1.0 mg/L NAA (MCBN) showed the highest number of plantlets at 15℃ at the second week of culture. At the second and third week of culture, MS medium supplemented with 2.0 mg/L BAP and 1.0 mg/L NAA (MBN) showed the best result in terms of the number of leaves and the longest leaves at 15℃ and 25℃, respectively. The present study showed evidence of successful in vitro propagation of B. auricomum Lindl.

Keywords

Acknowledgement

We would like to thank the Lifestyle Medicine Department of Jeonbuk National University for the technical support and providing the laboratory facilities used in this research.

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