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Investigation on the SARS-CoV-2 RNA and PM-2.5 inside Public Transportations in Seoul

서울시 대중교통 내 SARS-CoV-2 RNA와 PM-2.5 오염도 실증연구

  • Seo, Minjeong (Seoul Metropolitan Government Research Institute of Public Health and Environment) ;
  • Hong, Juhee (Seoul Metropolitan Government Research Institute of Public Health and Environment) ;
  • Rhee, Hojun (Seoul Metropolitan Government Research Institute of Public Health and Environment) ;
  • Park, Jinsol (Seoul Metropolitan Government Research Institute of Public Health and Environment) ;
  • Lim, Hakmyeong (Seoul Metropolitan Government Research Institute of Public Health and Environment) ;
  • Park, Myung Kyu (Seoul Metropolitan Government Research Institute of Public Health and Environment) ;
  • Min, Byungchul (Seoul Metropolitan Government Research Institute of Public Health and Environment) ;
  • Park, Eun Sun (Seoul Metropolitan Government Research Institute of Public Health and Environment) ;
  • Lee, Sanghoon (Seoul Metropolitan Government Research Institute of Public Health and Environment) ;
  • Kim, Hanjun (Seoul Metropolitan Government Research Institute of Public Health and Environment) ;
  • Ha, Kwangtae (Seoul Metropolitan Government Research Institute of Public Health and Environment) ;
  • Kwon, Seungmi (Seoul Metropolitan Government Research Institute of Public Health and Environment) ;
  • Shin, Jinho (Seoul Metropolitan Government Research Institute of Public Health and Environment) ;
  • Lee, Jaein (Seoul Metropolitan Government Research Institute of Public Health and Environment) ;
  • Hwang, Youngok (Seoul Metropolitan Government Research Institute of Public Health and Environment) ;
  • Oh, Younghee (Seoul Metropolitan Government Research Institute of Public Health and Environment) ;
  • Shin, Yongseung (Seoul Metropolitan Government Research Institute of Public Health and Environment)
  • 서민정 (서울특별시보건환경연구원) ;
  • 홍주희 (서울특별시보건환경연구원) ;
  • 이호준 (서울특별시보건환경연구원) ;
  • 박진솔 (서울특별시보건환경연구원) ;
  • 임학명 (서울특별시보건환경연구원) ;
  • 박명규 (서울특별시보건환경연구원) ;
  • 민병철 (서울특별시보건환경연구원) ;
  • 박은선 (서울특별시보건환경연구원) ;
  • 이상훈 (서울특별시보건환경연구원) ;
  • 김한준 (서울특별시보건환경연구원) ;
  • 하광태 (서울특별시보건환경연구원) ;
  • 권승미 (서울특별시보건환경연구원) ;
  • 신진호 (서울특별시보건환경연구원) ;
  • 이재인 (서울특별시보건환경연구원) ;
  • 황영옥 (서울특별시보건환경연구원) ;
  • 오영희 (서울특별시보건환경연구원) ;
  • 신용승 (서울특별시보건환경연구원)
  • Received : 2022.02.04
  • Accepted : 2022.04.01
  • Published : 2022.06.30

Abstract

Recently, passengers using public transport are concerned about the effects of COVID-19 and fine dust. Therefore, from February 2020 to February 2021, we investigated whether SARS-CoV-2 RNA was detected even after disinfection in 55 public transportation places visited by confirmed patients in Seoul. 34 air samples and 702 object surface samples were collected and tested with RT-PCR, one surface sample was positive. In addition, preemptive investigations were conducted in 22 subway trains that passengers were being on board at that time. 1,018 preemptive tests were performed, and all were negative. Although PM-2.5 is dangerous in itself, it can be a potential carrier of viruses. It seemed that a solution was needed as one line continuously exceeded the criteria of PM-2.5. Through this study, it is judged that cluster infection in public transportation can be prevented if efforts to reduce the concentration of fine dust, appropriate disinfection management, and personal disinfection such as wearing a mask in public transportations.

Keywords

Acknowledgement

서울특별시 서울산업진흥원 <서울글로벌챌린지 2021> 사업을 통해 우수 논문으로 선정되어 작성된 논문입니다.

References

  1. Biryukov, J., Boydston, J.A., Dunning, R.A., Yeager J.J., Wood, S., Reese, A.L., Ferris, A., Miller, D., Weaver, W., Zeitouni, N.E., Phillips, A., Freeburger, D., Idris Hooper, I., Shumate, S.R., Yolitz, J., Krause, M., Williams, G., Dawson, D.G., Herzog, A., Dabisch, P., Wahl, V., Hevey, M.C., and Altamura,L.A. (2020). Increasing temperature and relative humidity accelerates in- activation of SARS-CoV-2 on surfaces, mSphere, 5:e00441-20.
  2. CDC(Centers for Disease Control and Prevention). (2021). Coronavirus disease 2019 (COVID-19). How COVID-19 spreads https://www.cdc.gov/coronavirus/2019-ncov/prevent-getting-sick/how-covid-spreads.html
  3. Chin, A.W.H., Chu, J.T.S., Perera, M.R.A., Hui, K.P.Y., Yen, H., Chan, M.C.W., Peiris, M., Poon, L.L.M., (2020). Stability of SARS-CoV-2 in different environmental conditions, The Lancet Microbe, vol. 1, no. 1, p.e10. https://doi.org/10.1016/s2666-5247(20)30003-3
  4. Corman, V.M., Landt, O., Kaiser, M., Molenkamp, R., Meijer, A., Chu, D.K.W., Bleicker, T., Brunink, S., Schneider, J., Schmidt, M.L., Mulders, D.G., Haagmans, B.L., Veer, B., Brink, S., Wijsman, L., Goderski, G., Romette, J.L., Ellis, J., Zambon, M., Peiris, M., Goossens, H., Reusken, C., Koopmans, M.P.G., and Drosten, C. (2020). Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR. Euro Surveill, 25(3), 23-30.
  5. Doremalen, N., Bushmaker, T., Morris, D.H., Holbrook, M.G., Gamble, A., Williamson, B.N., Tamin, A., Harcourt, J.L., Thornburg, N.J., Gerber, S.I., Lloyd-Smith, J.O., Wit, E., and Munster, V.J. (2020) Aerosol and surface stability of SARS-CoV-2 as compared with SARS-CoV-1. N Engl J Med. 382:1564–7. https://doi.org/10.1056/NEJMc2004973
  6. Fears, A.C., Klimstra, W.B., Duprex, P., Hartman, A., Weaver, S.C., Plante, K.C., Mirchandani, D., Plante, J.A., Aguilar, P.V., Fernandez, D., Nalca, A., Totura, A., Dyer, D., Kearney, B., Lackemeyer, M., Bohannon, J.K., Johnson, R., Garry, R.F., Reed, D.S., and Roy, C.J. (2020). medRxiv; doi: 10.1101/2020.04.13.20063784
  7. Kampf, G.,Todt, D.,. Pfaender, S., and Steinmann, E. (2020). Persistence of coronaviruses on inanimate surfaces and their inactivation with biocidal agents. J. Hosp. Infect. 104, 246&# 8211;251. https://doi.org/10.1016/j.jhin.2020.01.022
  8. Kampf, G., Lemmen, S., and Suchomel, M. (2021a) Ct values and infectivity of SARS-CoV-2 on surfaces. Lancet Infectious Diseases. Vol21, e141. https://doi.org/10.1016/S1473-3099(20)30883-5
  9. Kampf, G., Pfaender, S., Goldman. E., and Steinmann, E. (2021b). SARS-CoV-2 Detection Rates from Surface Samples Do Not Implicate Public Surfaces as Relevant Sources for Transmission, Hygiene, 1(1), 24–40. https://doi.org/10.3390/hygiene1010003
  10. Kim, S.H., Chang, S.Y., Sung, M., Park, J.H., Kim, H.B., Lee, H., Choi, J.P., Choi, W.S., and Min, J.Y. (2016). Extensive Viable Middle East Respiratory Syndrome(MERS) Coronavirus Contamination in Air and Surrounding Environment in MERS Isolation Wards. Clinical Infectious Diseases, 63(3), 363–369. https://doi.org/10.1093/cid/ciw239
  11. KCDC(Korea Centers of Disease Control and Prevention). (2021). Korea Coronavirus Disease-19, Republic of Korea, Media Resources, Press release, http://ncov.mohw.go.kr/en/tcmBoardList.do?brdId=12&brdGubun=125&data-Gubun=&ncvContSeq=&contSeq=&board_id=&gubun=
  12. KCDC(Korea Centers of Disease Control and Prevention). (2020). COVID-19 Disinfection Guidelines for Public-use Facilities and Multi-use Facilities 2020. 3-4thed. http://www.kdca.go.kr/board/board.es?mid=a20507020000&bid=0019
  13. Lv, J., Yang, J., Xue, J., Zhu, P., Liu, L., and Li, S. (2020). Detection of SARS-CoV-2 RNA residue on object surfaces in nucleic acid testing laboratory using droplet digital PCR. Science of The Total Environment, 742, 140370. https://doi.org/10.1016/j.scitotenv.2020.140370
  14. Lee, E.S., Park, M.B., Lee, T.J., Kim, S.D., Park, D.S., and Kim, D.S. (2016). Characterizing Particle Matter on the Main Section of the Seoul Subway Line-2 and Developing Fine Particle Pollution Map. Korean Society for Atmospheric Environment, 32(2), 216-232 https://doi.org/10.5572/KOSAE.2016.32.2.216
  15. Lee, E.S., Lee, T.J., Park, M.B., Lee, T.J., Park, D.S., and Kim, D.S. (2017). Characteristics of particle matter concentration and classification of contamination patterns in the Seoul metropolitan subway tunnels. 33(6), 593-604 https://doi.org/10.5572/KOSAE.2017.33.6.593
  16. Moreno, T., Pinto, R.M., Bosch, A., Moreno, N., Alastuey, A., Minguillon, M.C., Anfruns-Estrada, E., Guix, S., Fuentes, C., Buonanno, G., Stabile, L., Morawska, L., Querola, X. (2020). Tracing surface and airborne SARS-CoV-2 RNA inside public buses and subway trains. Environment International, 147, 106326
  17. Moriarty, L.F., Plucinski, M.M., Marston, B.J., Kurbatova, E.V., Knust, B., Murray, E.L,, Pesik, N., Rose, D., Fitter, D., Kobayashi, M., Toda, M., Cantey, P.T., Scheuer, T., Halsey, E.S., Cohen, N.J., Stockman, L., Wadford, D.A., Medley, A.M., Green, G., Regan, J.J., Tardivel, K., White, S., Brown, C., Morales, C., Yen, C., Wittry, B., Freeland, A., Naramore, S., Novak, R.T., Daigle, D., Weinberg, M., Acosta, A., Herzig, C., Kapella, B.K., Jacobson, K.R., Lamba, K., Ishizumi, A., Sarisky, J., Svendsen, E., Blocher, T., Wu, C., Charles, J., Wagner, R., Stewart, A., Mead, P.S., Kurylo, E., Campbell, S., Murray, R., Weidle, P., Cetron, M., and Friedman, C.R. (2020). Public health responses to COVID-19 outbreaks on cruise ships-Worldwide, February–March. Morbidity and Mortality Weekly Report(MMWR). 69(12), 347–352. https://doi.org/10.15585/mmwr.mm6912e3
  18. Ministry of Environment. (2020a). Enforcement Rules of the Indoor Air Quality Management Act(abbrevi- ation: Enforcement Rules of the Indoor Air Quality Act). Ministry of Environment Ordinance No. 918
  19. Ministry of Environment. (2020b). Manufacture and operation management guidelines for public transportation vehicles for indoor air quality management. Ministry of Environment Notice No. 2020-64, 2020
  20. Norefrina, S.M.N., Chee, W.Y., Nazlina, I., Mohd, H.J., Zetti, Z.R., Norlaila, M., Haris, H.A.H., Kuhan, C., Mohd, T.L., Phei, E.S., Chin, Y.L., Kemal, M.A., Jamal, H.H., and Mohd, S.M.N. (2021). Particulate matter (PM2.5) as a potential SARS-CoV-2 carrier. Scientific Reports, 2508.
  21. Rahmani, A.R., Leili, M., Azarian, G., Poormohammadi, A. (2020). Sampling and detection of corona viruses in air: A mini review. Science of the Total Environment, 740, 1-8.
  22. Seo, M., Lim, H., Park, M., Ha, G., Kwon, S., Shin, J., Lee, J., Hwang, Y., Oh, Y., and Shin, Y. (2021) Field study of the indoor environments for pre- venting the spread of the SARS-CoV-2 in Seoul. Indoor Air. 2021;00:1–13.
  23. Seoul. (2020) Seoul transportation in 2020. ISBN : 9791165992736. 51-6110000-002504-01
  24. Seoul. (2021). Major traffic statistics. https://news.seoul.go.kr/traffic/archives/345
  25. United Nations COVID-19 Response. How does COVID-19 spread?. (2020). https://www.un.org/en/coronavirus/covid-19-faqs
  26. Vania, A.V., Bruno, P.R.L., Maria, E.G., Caroline, P.B., Eduardo, B., Viviane de, S.G.F., Meri, B.N., Sonia, M.R., Katherine, A.T.C., Izadora, C.F., Morgana, F.V., Ramiro, G.E., Jacques, F.M., Vanete, T.S., and Emanuel, M.S. (2021). Environmental Detection of SARS-CoV-2 Virus RNA in Health Facilities in Brazil and a Systematic Review on Contamination Sources. Int. J. Environ. Res. Public Health, 18, 3824.
  27. Virtanen, J., Aaltonen, K., Kivisto, I., and SironenJenni, T. (2021). Survival of SARS-CoV-2 on Clothing Materials. Hindawi Advances in Virology Volume 2021, Article ID 6623409.
  28. Wang, D., Hu, B., Hu, C., Zhu, F., Liu, X., Zhang, J., Wang, B., Xiang, H., Cheng, Z., Xiong, Y., Zhao, Y., Li, Y., Wang, X., and Peng, Z. (2020). Clinical characteristics of 138 hospitalized patients with 2019 novel coronavirus-infected pneumonia in Wuhan, China. JAMA, 323, 1061–1069. https://doi.org/10.1001/jama.2020.1585
  29. WHO(World Health Organization). (2020). Surface sampling of coronavirus disease (COVID-19): A practical "how to" protocol for health care and public health professionals. https://www.wh-o.int/publications/i/item/surface-sampling-of-coronavirus-dis-ease-(-covid-19)-a-practical-how-to-proto-col-for-health-care-and-public-health-professionals
  30. Wolfel, R., Corman, V.M., Guggemos, W., Seilmaier, M., Zange, S., Muller, M.A., Niemeyer, D., Jones, T.C., Vollmar, P., Rothe, C., Hoelscher, M., Bleicker, T., Brunink, S., Schneider, J., Ehmann, R., Zwirglmaier, K., Drosten, C., and Wendtner, C. (2020). Virological assessment of hospitalized patients with COVID-2019. Nature, 581, 465–469 https://doi.org/10.1038/s41586-020-2196-x
  31. Yun, S.J., Song, M.Y., Lee, J.H., and Chun, H.J. (2020). Performance assessment of air purification system on buses in Seoul. Seoul Institute of Technology, 2020-SR-05