Journal of Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Method validation of marker compounds from Angelicae Dahuricae Radix as functional food ingredients

건강기능식품 원료로서 구릿대의 지표성분 분석법 검증

  • Bo-Ram, Choi (Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA) ;
  • Dahye, Yoon (Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA) ;
  • Hyeon Seon, Na (Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA) ;
  • Geum-Soog, Kim (Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA) ;
  • Kyung-Sook, Han (Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA) ;
  • Sookyeong, Lee (National Agrobiodiversity Center, National Institute of Agricultural Sciences, RDA) ;
  • Dae Young, Lee (Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA)
  • Received : 2022.11.14
  • Accepted : 2022.11.19
  • Published : 2022.12.31
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Abstract

This study was performed to establish an analytical method for the standardization of Angelicae Dahuricae Radix as a functional ingredient. We established six compounds including oxypeucedanin hydrate (1), byakangelcol (2), oxypeucedanin (3), imperatorin (4), phellopterin (5) and isoimperatorin (6) as marker compounds of Angelicae Dahuricae Radix. An analytical method using Ultra Performance Liquid Chromatography (UPLC) was established and validated for marker compounds of Angelicae Dahuricae Radix. The specificity was confirmed by the chromatogram from UPLC and the value of coefficient determination was also higher than 0.999, indicating high linearity. The relative standard deviation (RSD) and recovery of marker compounds were less than 5% and in the range of 90- 110%, respectively, which means that this method has high accuracy and precision. Therefore, this analytical method could be used as basic data for the development of functional ingredients for health functional food of Angelicae Dahuricae Radix.

백지는 산형과(Umbelliferae)에 속하는 구릿대(Angelica dahurica)의 뿌리를 건조한 것으로, 다양한 약리작용을 하는 것으로 알려져 예로부터 한약재로 사용되어 왔다. 백지의 대표적인 활성 성분인 coumarin을 포함하여 다양한 성분이 알려져 있으며, 최근 백지의 다양한 생리활성이 보고되고 있다. 본 연구는 백지를 건강기능식품의 기능성 원료로 활용하고자, 백지의 지표성분 설정 및 이에 대한 UPLC 분석법을 확립하여 분석법 유효성 검증을 실시하였다. 백지의 지표성분으로는 oxypeucedanin hydrate, byakangelicol, oxypeucedanin, imperatoin, phellopterin, isoimperatorin의6종을 설정하였다. 분석법 유효성 검증은 특이성, 직선성, 검출한계, 정량한계, 정확성, 정밀성 등의 항목을 이용하여 확인하였다. 본 시험법에서 지표성분 6종은 서로 간섭없이 분리되어 특이성을 확인하였고, 결정계수의 값도 0.999 이상으로 나타나 높은 직선성을 나타내었다. 분석법 유효성 검증 결과, 지표 성분 6종의 RSD는 5% 이하로 나타났으며 90-110%의 범위의 회수율을 나타내 높은 정확성과 정밀성이 있는 것을 확인할 수있었다. 이러한 결과는 UPLC를 이용한 백지의 지표성분 분석법이, 새롭게 제시한 6종에 대해 적합한 동시분석 분석법임을 나타낸다. 따라서, 본 연구를 통하여 확립된 백지의 지표성분 분석법이 향후 백지(구릿대)의 건강기능식품 기능성 원료 개발을 위한 기초자료로 활용될 것으로 기대된다.

Keywords

Acknowledgement

본 논문은 농촌진흥청 연구사업 (과제번호: PJ01422702)의 지원에 의해 이루어진 것으로 이에 감사드립니다.

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