Journal of Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Combinatorial effects of arginine and n-hexane extract from Korean red ginseng marc against Streptococcus mutans

Streptococcus mutans에 대한 홍삼박 n-hexane 추출물과 Arginine의 병용 효과

  • Dong Chung, Kim (Department of Chemical Engineering, Chungwoon University) ;
  • Man-Jin, In (Department of Chemical Engineering, Chungwoon University)
  • Received : 2022.10.19
  • Accepted : 2022.11.14
  • Published : 2022.12.31
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Abstract

In this study, the effect of the combined use of a lipid-soluble n-hexane extract of red ginseng marc (HERGM) and water-soluble arginine, which exhibits anticaries activity, on the growth of Streptococccus mutans was investigated. As a result of checkerboard assay, HERGM and arginine showed a synergistic effect in inhibiting the growth of S. mutans with a fractional inhibitory concentration index of 0.396. Combination treatments of HERGM and arginine resulted in leakage of nucleic acid components and decrease in the viable cell counts of S. mutans, all of which were proportional to the concentration of HERGM. In conclusion, the synergistic effect of HERGM and arginine on the growth inhibition of S. mutans is mainly attributed to HERGM.

본 연구에서는 항충치 활성을 보이는 지용성의 홍삼박 n-hexane 추출물과 수용성의 arginine의 병용이 S. mutans의 생육에 미치는 영향을 조사하였다. Checkerboard assay로 분석한 결과, 홍삼박 n-hexane 추출물과 arginine은 fractional inhibitory concentration index 0.396으로 S. mutans의 생육 저해에 시너지 효과를 나타내었다. 홍삼박 n-hexane 추출물과 arginine의 병용은 핵산 성분의 유출과 생균수의 감소를 초래하였으며, 이는 모두 홍삼박 n-hexane 추출물의 농도에 비례하였다. 결론적으로 S. mutans의 생육 저해에 대한 홍삼박 n-hexane 추출물과 arginine의 시너지 효과는 주로 홍삼박 n-hexane 추출물이 기여하는 것으로 판단된다.

Keywords

Acknowledgement

본 연구는 2022년 청운대학교 학술연구조성비의 지원으로 수행 되었습니다.

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