Journal of Species Research

The National Institute of Biological Resources (국립생물자원관)
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New record of Pleuronema marinum Dujardin, 1841 (Protozoa, Ciliophora) from South Korea

  • Jeong Hyeon, Yeo (Department of Biology, Gangneung-Wonju National University) ;
  • Ji Hye, Choi (Department of Biology, Gangneung-Wonju National University) ;
  • Atef, Omar (Department of Zoology, Al Azhar University) ;
  • Jae-Ho, Jung (Department of Biology, Gangneung-Wonju National University)
  • Received : 2022.07.13
  • Accepted : 2022.08.09
  • Published : 2022.11.30
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Abstract

During a field survey of Korean marine ciliates, we collected Pleuronema marinum from a brackish water sample. It is characterized by the presence of a contractile vacuole in mid-body, rather than the subterminal/ terminal contractile vacuole as in other congeners. The cells were examined in vivo and based on protargol and 'wet' silver nitrate impregnation. In addition, the nuclear 18S rRNA gene was sequenced using a single cell. The Korean population morphologically and molecularly resembles a Chinese population of P. marinum. Historical review of the species concludes that 1) two or more species have been assigned into P. marinum, 2) the position of contractile vacuole (e.g., in mid-body) is a valid character state, and 3) P. marinum is probably a rare species. Here we provide a monographic treatment of P. marinum to clarify the issue and for further studies of relevant species. Considering there are about 40 nominal species and complex nomenclatural acts in the genus Pleuronema, further studies should provide descriptions based on protargol and 'wet' silver impregnation with marker gene(s).

Keywords

Acknowledgement

This work was supported by grants from the National Institute of Biological Resources (NIBR), funded by the Ministry of Environment (MOE) of the Republic of Korea (NIBR202002204, NIBR202231206).

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