Acknowledgement
We thank Dr. Kang, J. C., Dr. Yang, M. Y. and members of the Molecular Phylogeny of Marine Algae Laboratory at Jeju National University for helping us to collect samples. This study was supported by the 2022 education, research and student guidance grant funded by Jeju National University.
References
- Bae, E.H. 2010. Ulothrichales, Ulvales. In: Algal flora of Korea. Volume 1, Number 1. Chlorophyta: Ulvophyceae: Ulothrichales, Ulvales, Cladophorales, Bryopsidales. Marine green algae. (Bae, E. H., Kim, H.-S., Kwon, C.-J., Hwang, I.-K., Kim, G. H. & Klochkova, T. A. Eds), pp. 7-52. Incheon: National Institute of Biological Resources.
- Chihara, M. 1969. Ulva arasakii, a new species of green algae: its life history and taxonomy. Bulletin of the National Science Museum, Tokyo, Series B(Botany) 12:849-862.
- Cormaci, M., G. Furnari and G. Alongi. 2014. Flora marina bentonica del Mediterraneo: Chlorophyta. Bolletino dell' Accademia Gioenia di Scienze Naturali di Catani 47:11-436.
- Fort, A., M. McHale, K. Cascella, P. Potin, M. Rerrineau, P.D. Kerrison, E. da Coasta, R. Calado, M. do Rosario Domingues, I.C. Azevedo, I. Sousa-Pinto, C. Gachon, A. van der Werf, W. de Visser, J.E. Beniers, H. Jansen, M.D. Guiry and R. Sulpice. 2022. Exhaustive reanalysis of barcode sequences from public repositories highlights ongoing misidentifications and impacts taxa diversity and distribution. Mol. Ecol. Resour. 22:86-101. https://doi.org/10.1111/1755-0998.13453
- Fort, A., M. McHale, K. Cascella, P. Potin, B. Usadel, M.D. Guiry and R. Sulpice. 2021. Foliose Ulva species show considerable inter-specific genetic diversity, low intra-specific variation, and rare occurrence of inter-specific hybrid in the wild. J. Phycol. 57:219-233. https://doi.org/10.1111/jpy.13079
- Guiry, M.D. and G.M. Guiry. 2022. AlgaeBase. World-wide electronic publication, National University of Ireland, Galway [Available from: http://www.algaebase.org. Accessed on January 30, 2022].
- Hall, T.A. 1999. BioEdit: a user-friendly biological alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp. Ser. 41:95-98.
- Hanyuda, T., S. Arai and K. Ueda. 2000. Variability in the rbcL Introns of Caulerpalean Algae (Chlorophyta, Ulvophyceae). J. Plant Res. 113:403-413. https://doi.org/10.1007/PL00013948
- Hayden, H.S., J. Blomster, C.A. Maggs, P.C. Silva, M.J. Stanhop and R. Waaland. 2003. Linnaeus was right all along: Ulva and Enteromorpha are distinct genera. Eur. J. Phycol. 38:277-294. https://doi.org/10.1080/1364253031000136321
- Heesch, S., J.E.S. Broom, K.F. Neill, T.J. Farr, J.L. Dalen and W.A. Nelson. 2009. Ulva, Umbraulva and Gemina: genetic survey of New Zealand taxa reveals diversity and introduced species. Eur. J. Phycol. 44:143-154. https://doi.org/10.1080/09670260802422477
- Heesch, S., J.E. Sutherland, S. Ringham and W.A. Nelson. 2021. Ulva L.(Ulvales, Chlorophyta) from Manawatawhi/Three Kings Islands, New Zealand: Ulva piritoka Ngati Kuri, Heesch & W.A. Nelson sp. nov. and records of two non-native species, U. compressa and U. rigida. Cryptogam., Algol. 42:129-140.
- Hiraoka, M., S. Shimada, M. Uenosono and M. Masuda. 2004. A new green-tide-forming alga, Ulva ohnoi Hiraoka et Shimada sp. nov. (Ulvales, Ulvophyceae) from Japan. Phycol. Res. 52:17-29. https://doi.org/10.1111/j.1440-1835.2003.00321.x
- Hughey, J.R., C.A. Maggs, F. Mineur, C. Jarvis, K.A. Miller, S.H. Shabaka and P.W. Gabrielson. 2019. Genetic analysis of the Linnaean Ulva lactuca (Ulvales, Chlorophyta) holotype and related type specimens reveals name misapplications, unexpected origins, and new synonymies. J. Phycol. 55:503-508. https://doi.org/10.1111/jpy.12860
- Hughey, J.R., P.W. Gabrielson, C.A. Maggs and F. Mineur. 2022. Genomic analysis of the lectotype specimens of European Ulva rigida and Ulva lacinulata (Ulvaceae, Chlorophyta) reveals the ongoing misapplication of names. Eur. J. Phycol. 52:143-153.
- Hughey, J.R., P.W. Gabrielson, C.A. Maggs, F. Mineur and K.A. Miller. 2021. Taxonomic revisions based on genetic analysis of type specimens of Ulva conglobata, U. laetevierns, U. pertusa and U. spathulata (Ulvales, Chlorophyta). Phycol. Res. 69:148-153. https://doi.org/10.1111/pre.12450
- Ichihara, K., K. Miyaji and S. Shimada. 2013. Comparing the low-salinity tolerance of Ulva species distributed in different environments. Phycol. Res. 61:52-57. https://doi.org/10.1111/j.1440-1835.2012.00668.x
- Kang, J.H., J.E. Jang, J.H. Kim, S.Y. Byeon, S. Kim, S.K. Choi, Y.H. Kang, S.R. Park and H.J. Lee. 2019. Species composition, diversity, and distribution of the genus Ulva along the coast of Jeju Island, Korea based on molecular phylogenetic analysis. PLoS ONE 14(7):e0219958. https://doi.org/10.1371/journal.pone.0219958.
- Kawai, H., T. Hanyuda, I. Mine, S. Takaichi, R. Terada and T. Kitayama. 2020. Morphology and molecular phylogeny of Umbraulva spp. (Ulvales, Ulvophyceae), and proposal of Ryuguphycus gen. nov. and R. kuaweuweu comb. nov. Eur. J. Phycol. 56(1). https://doi.org/10.1080/09670262.2020.1753815.
- Kirkendale, L., G.W. Saunders and P. Winberg. 2013. A molecular survey of Ulva (Chlorophyta) in temperate Australia reveals enhanced levels of cosmopolitanism. J. Phycol. 49:69-81. https://doi.org/10.1111/jpy.12016
- Koeman, R.P.T. and C. van den Hoek. 1981. The taxonomy of Ulva (Chlorophyceae) in the Netherlands. Br. Phycol. J. 16:9-53. https://doi.org/10.1080/00071618100650031
- Kraft, L.G.K., G.T. Kraft and R.F. Waller. 2010. Investigations into southern Australian Ulva (Ulvophyceae, Chlorophyta) taxonomy and molecular phylogeny indicate both cosmopolitanism and endemic cryptic species. J. Phycol. 46:1257-1277. https://doi.org/10.1111/j.1529-8817.2010.00909.x
- Krupnik, N., G. Paz, J. Douek, E. Lewinsohn, N. Carmel, F. Mineur and C.A. Maggs. 2018. Native, invasive and cryptogenic Ulva species from Israeli Mediterranean Sea: risk and potential. Mediterranean Marine Science 19:132-146. https://doi.org/10.12681/mms.2104
- Lee, H.W., E.H. Bae and M.S. Kim. 2020. Umbraulva yunseulla sp. nov. (Ulvaceae, Chlorophyta) from a subtidal habitat of Jeju Island. Algae 35:349-359. https://doi.org/10.4490/algae.2020.35.12.3
- Lee, H.W., J.C. Kang and M.S. Kim. 2019. Taxonomy of Ulva causing from Jeju Island, Korea with new species, U. pseudo-ohnoi sp. nov. (Ulvales, Chlorophyta). Algae 34:253-266. https://doi.org/10.4490/algae.2019.34.12.9
- Longhnane, C.J., L.M. McIvor, F. Rindi, D.B. Stengel and M.D. Guiry. 2008. Morphology, rbcL phylogeny and distribution of distromatic Ulva (Ulvophyceae, Chlorophyta) in Ireland and southern Britain. Phycologia 47:416-429. https://doi.org/10.2216/PH07-61.1
- Manhart, J.R. 1994. Phylogenetic analysis of green plant rbcL sequences. Mol. Phylogenet. Evol. 3:114-127. https://doi.org/10.1006/mpev.1994.1014
- Matsumoto, K. and S. Shimada. 2015. Systematics of green algae resembling Ulva conglobata, with a description of Ulva adhaerens sp. nov. (Ulvales, Ulvophyceae). Eur. J. Phycol. 50:100-111. https://doi.org/10.1080/09670262.2014.994189
- Matsumoto, T., F. Shinozaki, T. Chikuni, A. Yabuki, K. Takishita, M. Kawachi, T. Nakayama, I. Inouye, T. Hashimoto and Y. Inagaki. 2011. Green-colored plastids in the dinoflagellate genus Lepidodinium are of Core Chlorophyte Origin. Protist 162:268-276. https://doi.org/10.1016/j.protis.2010.07.001
- Miladi, R., A. Manghisi, S.A. Minicante, G. Genovese, S. Abdelkafi and M. Morabito. 2018. A DNA barcoding survey of Ulva (Chlorophyta) in Tunisia and Italy reveals the presence of the overlooked alien U. ohnoi. Cryptogam. Algol. 39:85-107. https://doi.org/10.7872/crya/v39.iss1.2018.85
- Monotilla, A.P., T. Nishimura, M. Adachi, Y. Tanii, D.B. Largo and M. Hiraoka. 2018. Examination of prezygotic and postzygotic isolating barriers in tropical Ulva (Ulvophyceae, Chlorophyta): evidence for ongoing speciation. J. Phycol. 54:539-549. https://doi.org/10.1111/jpy.12755
- National Institute of Biological Resources. 2019. National Species List of Korea. I. Plants, Fungi, Algae, Prokaryotes. Designzip. 924 pp. [in Korea].
- National Marine Biodiversity Institute of Korea. 2022. 2022 National List of Marine Species. III. Marine Plants. Namuprint. 99 pp. [in Korea].
- Rambaut, A. 2012. FigTree, tree figure drawing tool v1.4.0. Institute of Evolutionary Biology, University of Edinburgh [Available from: http://tree.bio.ed.ac.uk/software/figtree/, Accessed on May 30, 2018].
- Ronquist, F. and J.P. Huelsenbeck. 2003. Mr Bayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 19:1572-1574. https://doi.org/10.1093/bioinformatics/btg180
- Saunders, G.W. and H. Kucera. 2010. An evaluation of rbcL, tufA, UPA, LSU and ITS as DNA barcode markers for the marine green macroalgae. Cryptogam. Algol. 31:487-528.
- Setchell, W.A. and N.L. Gardner. 1920. The marine algae of the Pacific coast of North America. Part II. Chlorophyceae. University of California Publications in Botany 8: 139-374, pls. 9-33.
- Shao, Z., W. Li, H. Guo and D. Duan. 2015. Isolation, expression and characterization of rbcL gene from Ulva prolifera J. Agardh (Ulvophyceae, Chlrophyta). J. Ocean Univ. China 14:1087-1095. https://doi.org/10.1007/s11802-015-2661-6
- Shimada, S., M. Hiraoka, S. Nabata, M. Lima and M. Masuda. 2003. Molecular phylogenetic analyses of the Japanese Ulva and Enteromorpha (Ulvales, Ulvophyceae), with special reference to the free-floating Ulva. Phycol. Res. 51:99-108. https://doi.org/10.1046/j.1440-1835.2003.00296.x
- Spalding, H.L., K.Y. Conklin, C.M. Smith, C.J. O'Kelly and A.R. Sherwood. 2016. New Ulvaceae (Ulvophyceae, Chlorophyta) from mesophotic ecosystems across the Hawaiian Archipelago. J. Phycol. 52:40-53. https://doi.org/10.1111/jpy.12375
- Stamatakis, A. 2006. RAxML-VI-HPC: maximum likelihood-based phylogenetic analyses with thousands of taxa and mixed models. Bioinformatics 22:2688-2690. https://doi.org/10.1093/bioinformatics/btl446
- Steinhagen, S., R. Karez and F. Weinberger. 2019. Cryptic, alien and lost species: molecular diversity of Ulva sensu lato along the German coasts of the North and Baltic Seas. Eur. J. Phycol. 54:466-483. https://doi.org/10.1080/09670262.2019.1597925
- Tamura, K., D. Peterson, N. Peterson, G. Stecher, M. Nei and S. Kumar. 2011. MEGA5: Molecular Evolutionary Genetic Analysis using Maximum Likelihood, Evolutionary Distance, and Maximum Parsimony Methods. Mol. Biol. & Evol. 28:2731-2739. https://doi.org/10.1093/molbev/msr121
- Tanner, C.E. 1986. Investigations of the taxonomy and morphological variation of Ulva (Chlorophyta): Ulva californica Wille. Phycologia 25:510-520. https://doi.org/10.2216/i0031-8884-25-4-510.1
- Zhigadlova, G.G. and N.A. Lopatina. 2020. [Green algae Chlrophyta algae]. In: Flora and Fauna of Matua Island(middle Kuril Islands): the field guide. Volume 1. Sea. (Sanamyan, K. E. & Sanamyan, N. P. Eds), pp. 468-485. Cherepovets: Intron [in Russian].