대한한의학방제학회지 (Herbal Formula Science)

  • 제30권4호
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  • Pages.259-267
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  • 2022
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  • 1229-1218(pISSN)
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  • 2288-5641(eISSN)
대한한의학방제학회 (The Korean Medicine Society for the Herbal Formula Study)
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ICR 마우스를 이용한 오미자박 추출물의 소핵 시험

Erythrocyte Micronucleus Test of Pomace Schisandra chinensis Extracts Using ICR Mouse

  • 투고 : 2022.11.04
  • 심사 : 2022.11.14
  • 발행 : 2022.11.30
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초록

Objectives : In this study, erythrocyte micronucleus test of pomace Schisandra chinensis extracts was conducted in order to up-cycling to a high value-added industry using by-products discarded in the production process of Schisandra chinensis products and active ingredients such as dibenzocyclooctadiene lignans in Schisandra chinensis. Methods : The micronucleus test was performed according to the 'OECD Guidelines'. Including the negative control group(0 mg/kg) and the positive control group(CPA 70 mg/kg), pomace Schisandra chinensis extracts were orally administered to ICR mouse at doses of 500, 1,000, and 2,000 mg/kg. After sacrificing the experimental animals bone marrow cells were collected and micronucleated polychromatic erythrocyte were counted. And genetic toxicity was confirmed according to the frequency of micronucleus. Results : As a result of the micronucleus test, there were no changes in body weight, clinical signs, or death in any group. But, a significant increase was observed in the frequency of micronucleated polychromatic erythrocyte among polychromatic erythrocytes in the positive control group administered with CPA compared to the negative control group(p<0.05). Whereas, no significant increase was observed in the group administered with pomace Schisandra chinensis extracts compared to the negative control group. Conclusions : Pomace Schisandra chinensis extracts did not induce micronucleus in bone marrow cells of ICR mouse up to a concentration of 2,000 mg/kg, and it was judged that no genetic toxicity was observed.

키워드

과제정보

본 결과물은 환경부의 재원으로 한국환경산업기술원의 야생생물 유래 친환경 신소재 및 공정기술개발사업(과제번호 : 2021003270008)의 지원을 받아 연구되었습니다.

참고문헌

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