Herbal Formula Science (대한한의학방제학회지)

The Korean Medicine Society for the Herbal Formula Study (대한한의학방제학회)
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Effect of water extract Phellinus linteus-discard Schisandra chinensis solid fermented extracts in an Animal Model of Dexamethasone-Induced Muscle Loss

Dexamethasone으로 유도한 근감소 동물모델에서 상황버섯-오미자박 고상발효 열수추출물의 근감소 개선에 대한 효과

  • Su-Jin, Hwang (Department of Physiology, College of Korean Medicine Dongguk University) ;
  • Young-Suk, Kim (Glucan Co. Ltd.) ;
  • Tae Woo, Oh (Korean Medicine (KM)-Application Center, Korea Institute of Oriental Medicine (KIOM))
  • 황수진 (동국대학교 한의과대학, 한방생리학교실) ;
  • 김영숙 ((주)글루칸) ;
  • 오태우 (한국한의학연구원 한의기술응용센터)
  • Received : 2022.11.06
  • Accepted : 2022.11.13
  • Published : 2022.11.30
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Abstract

Objectives : In this study, it was investigated the effects of solid-phase fermentation extraction with Phellinus linteus of discarded Schisandra chinensis extract (PS) and its action mechanism on dexamethasone-induced muscle atrophy in mice. Methods : In mice, muscle atrophy model was induced by dexamethasone (5 mg/kg, I.p) once daily for 2 weeks and with PS extract administration (100 and 300 mg/kg, p.o.) as treatment groups. The changes in body weights, grip strength, Treadmill test, muscle weights, and the expression of atrophy-related genes were measured in muscle atrophy mice. The histological changes of gastrocnemius tissues were also observed by H&E staining with measurement of myofiber size. Results : The administration of PS extract increased significantly body weights, grip strength, treadmill test and muscle weights in muscle atrophy mice. PS extract administration increased significantly the area of myofibers and inhibited structural damages of muscle and increased significantly the expression of myogenin and decreased significantly the expression of MuRF1, Atrogin1 and phosphorylation of AMPK and PGC1α in muscle tissues of muscle atrophy mice. Conclusions : These results indicate that PS extract has a improvement effects on muscle atrophy with stimulation of myogenic differentiation and inhibition of mRNA degradation that could be related with the activation of AMPK and PGC1α signaling pathways in muscle. This suggests that PS extract can apply to treat muscle atrophy in clinics.

Keywords

Acknowledgement

본 연구는 환경부의 재원으로 한국환경산업기술원의 야생생물 유래 친환경 신소재 및 공정기술개발사업의 지원을 받아 연구되었습니다. (RE202101318, 한국한의학연구원 ERTERT2112080).

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