The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology (한방안이비인후피부과학회지)

The Society of Korean Medicine Ophthalmology, Otolaryngology & Dermatology (대한한방안이비인후피부과학회)
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Anti-aging and Anti-oxidative Effect of Naesohwangryun-tang(NSHRT)

내소황련탕(內疎黃連湯)의 항노화 및 항산화 효능 연구

  • Oh, Sol-Ra (Department Of Cosmetic Science, Semyung University) ;
  • Nam, Sang-Woo (Department Of Cosmetic Sciences & Beauty Biotechnology, Semyung University) ;
  • Song, Young-Chae (Department Of Korean Medical Ophtalmology & Otolaryngology & Dermatology, College of Korean Medicine, Semyung University) ;
  • Kim, Hee-Taek (Department Of Korean Medical Ophtalmology & Otolaryngology & Dermatology, College of Korean Medicine, Semyung University) ;
  • Kim, Yong-Min (Department Of Cosmetic Sciences & Beauty Biotechnology, Semyung University) ;
  • Lee, Chun-Il (Department Of Cosmetic Sciences & Beauty Biotechnology, Semyung University)
  • 오솔라 (세명대학교 화장품과학과) ;
  • 남상우 (세명대학교 화장품뷰티생명공학부) ;
  • 송영채 (세명대학교 한의과대학 한방안이비인후피부과) ;
  • 김희택 (세명대학교 한의과대학 한방안이비인후피부과) ;
  • 김용민 (세명대학교 화장품뷰티생명공학부) ;
  • 이천일 (세명대학교 화장품뷰티생명공학부)
  • Received : 2021.10.01
  • Accepted : 2021.11.09
  • Published : 2021.11.25
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Abstract

Objectives : This study is to investigate the anti-aging and antioxidant effects of Naesohwangryeon-tang(NSHRT) extract in Hs68 fibroblasts. Methods : The fibroblast cell line Hs68 cells were used and MTT analysis was performed to measure the cell viability of Naesohwangryeon-tang, and then MMP-1 was induced with the cytokine TNF-𝛼 to confirm the anti-aging effect through real-time PCR and ELISA. In addition, ROS was induced with the TNF-𝛼 to confirm the anti-oxidant effect through DCF-DA. The signaling pathway according to the antioxidant effect was performed by western blot. Results : Naesohwangryeon-tang did not show cytotoxicity at all concentrations(100-800㎍/㎖) treated on Hs68 cells, and the inhibitory effect of MMP-1 was also confirmed. In addition, it was confirmed that the intracellular ROS increased by TNF-α was decreased by Naesohwangryeon-tang, and it was confirmed that the expression of NQO1 was increased in a concentration-dependent manner of Naesohwangryeon-tang. Conclusions : These results suggest that Naesohwangryeon-tang can be used as an anti-aging material by demonstrating the anti-aging and antioxidant effects of Naesohwangryeon-tang.

Keywords

Acknowledgement

본 연구는 2021년도 세명대학교 대학혁신 지원사업에 의해 수행되었습니다.

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