The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology (한방안이비인후피부과학회지)

The Society of Korean Medicine Ophthalmology, Otolaryngology & Dermatology (대한한방안이비인후피부과학회)
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Skin Anti-aging and Anti-wrinkle Effects of Pinus koreaiensis Seed Oil

해송자 오일의 피부 항노화 및 주름 개선 효과

  • Kim, Hyung-Mook (Dept. of Manufacturing Pharmacy, College of pharmacy, Chungbuk National University) ;
  • Kim, Tae-Jun (Dept. of Korean Medicine, Ophthalmology, Otolaryngology & Dermatology, College of Korean Medicine, Semyung University) ;
  • Im, Dong-bin (Dept. of Korean Medicine, College of Korean Medicine, Semyung University) ;
  • Ha, Sun-Bong (Dept. of Korean Medicine, College of Korean Medicine, Semyung University) ;
  • Kim, Ee-Hwa (Dept. of Korean Medicine, College of Korean Medicine, Semyung University) ;
  • Cha, Byung-Sun (Dept. of Biological Sciences, College of Natural Sciences, Ajou University) ;
  • Heo, Hyo-Jin (Applied Biotechnology Program, Graduate School, Ajou University) ;
  • Brito, Sofia (Applied Biotechnology Program, Graduate School, Ajou University) ;
  • Lee, Yong-Moon (Dept. of Manufacturing Pharmacy, College of pharmacy, Chungbuk National University) ;
  • Bin, Bum-Ho (Dept. of Biological Sciences, College of Natural Sciences, Ajou University) ;
  • Kwak, Byeong-Mun (Dept. of Cosmetic Science and Beauty Biotechnology, Semyung University)
  • 김형묵 (충북대학교 약학부 응용약학과) ;
  • 김태준 (세명대학교 한의과대학 한방안이비인후피부과) ;
  • 임동빈 (세명대학교 한의과대학 한의학과) ;
  • 하순봉 (세명대학교 한의과대학 한의학과) ;
  • 김이화 (세명대학교 한의과대학 한의학과) ;
  • 차병선 (아주대학교 자연과학대학 생명과학과) ;
  • 허효진 (아주대학교 일반대학원 응용생명공학과) ;
  • 소피아브리토 (아주대학교 일반대학원 응용생명공학과) ;
  • 이용문 (충북대학교 약학부 응용약학과) ;
  • 빈범호 (아주대학교 자연과학대학 생명과학과) ;
  • 곽병문 (세명대학교 화장품뷰티생명공학부)
  • Received : 2021.10.02
  • Accepted : 2021.11.10
  • Published : 2021.11.25
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Abstract

Objectives : The aim of this study was to investigated the anti-aging and anti-wrinkles effects of the pinus koreaiensis seed(PKS) oil. Methods : The anti-oxidant effect was performed by beta-carotene bleaching assay and the intracellular proteome was analyzed expression of each 15 proteins by 2-D electrophoresis. And fatty acid was analysed by gas chromatography. Anti-wrinkle effect was analyzing human skin by the PRIMOS system. Results : Fatty acid analysis of PKS oil has shown oleic acid was 49.7% and linoleic acid was 34.1%. And the antioxidant effect was about 125% compared with alpha-tocoperol(0.1%) by beta carotene bleaching assay. In 2D PAGE analysis, fifteen protein changes in five mechanisms which was collagen synthesis pathway, MMPs, ECM-cell interaction, cytokine, antioxidant enzymes were analyzed. In case of anti-wrinkle effect was proved in vivo by analyzing human skin by the PRIMOS system. The analysis results of eye wrinkles for 4 weeks showed an improvement effect of over 6%. Conclusions : In this study, the amount of protein change in the five mechanism through the cell experiment and the skin anti wrinkle efficacy by the human in vivo test were investigated. As a result pinus koreaiensis seed oil by supercritical extraction could be used as a anti-aging and anti-wrinkle substance for the skin.

Keywords

Acknowledgement

본 연구는 2021학년도 세명대학교 대학혁신지원사업에 의한 연구임.

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