Mycobiology

The Korean Society of Mycology (한국균학회)
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Biodegradative Activities of Fungal Strains Isolated from Terrestrial Environments in Korea

  • Lee, Seung-Yeol (School of Applied Biosciences, College of Agriculture and Life Sciences, Kyungpook National University) ;
  • Ten, Leonid N. (School of Applied Biosciences, College of Agriculture and Life Sciences, Kyungpook National University) ;
  • Das, Kallol (School of Applied Biosciences, College of Agriculture and Life Sciences, Kyungpook National University) ;
  • You, Young-Hyun (Microorganism Resources Division, National Institute of Biological Resources) ;
  • Jung, Hee-Young (School of Applied Biosciences, College of Agriculture and Life Sciences, Kyungpook National University)
  • Received : 2021.01.18
  • Accepted : 2021.03.10
  • Published : 2021.06.30
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Abstract

Polylactic acid (PLA) and polycaprolactone (PCL) are commercially available bioplastics that are exploited worldwide, and both are biodegradable. The PLA and PCL polymer-degrading activity of 30 fungal strains that were isolated from terrestrial environments were screened based on the formation of a clear zone around fungal colonies on agar plates containing emulsified PLA or PCL. Among them, five strains yielded positive results of biodegradation. Strains Korean Agricultural Culture Collection (KACC) 83034BP and KNUF-20-PPH03 exhibited PCL degradation; two other strains, KACC 83035BP and KNUF-20-PDG05, degraded PLA; and the fifth strain, KACC 83036BP, biodegraded both tested plastics. Based on phylogenetic analyses using various combinations of the sequences of internal transcribed spacer (ITS) regions, RPB2, LSU, CAL, and b-TUB genes, the above-mentioned strains were identified as Apiotrichum porosum, Penicillium samsonianum, Talaromyces pinophilus, Purpureocillium lilacinum, and Fusicolla acetilerea, respectively. Based on our knowledge, this is the first report on (i) plastic biodegraders among Apiotrichum and Fusicolla species, (ii) the capability of T. pinophilus to degrade biodegradable plastics, (iii) the biodegradative activity of P. samsonianum against PCL, and (iv) the accurate identification of P. lilacinum as a PLA biodegrader. Further studies should be conducted to determine how the fungal species can be utilized in Korea.

Keywords

Acknowledgement

This work was supported by a grant from the National Institute of Biological Resources (NIBR), funded by the Ministry of Environment (MOE) of the Republic of Korea [NIBR202002104, NIBR202102109] and the Brain Pool Program (Grant No. 2020H1D3A2A01103925) through the National Research Foundation funded by the Ministry of Science and ICT, Republic of Korea.

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