대한한의학방제학회지 (Herbal Formula Science)

  • 제29권2호
  • /
  • Pages.93-103
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  • 2021
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  • 1229-1218(pISSN)
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  • 2288-5641(eISSN)
대한한의학방제학회 (The Korean Medicine Society for the Herbal Formula Study)
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녹내장 동물모델에서 단풍나무 잎 열수 및 에탄올 추출물의 안압 및 신경세포 보호 효과

Intraocular Pressure and Neuroprotective Effect of water and ethanol extracts from Leaves of Acer palmatum thumb. of glaucoma in DBA2/J mice

  • 오태우 (한국한의학연구원 한의기술응용센터) ;
  • 박광일 (경상국립대학교 수의과대학) ;
  • 마진열 (한국한의학연구원 한의기술응용센터)
  • Oh, Tae Woo (Korean Medicine (KM)-Application Center, Korea Institute of Oriental Medicine (KIOM)) ;
  • Park, Kwang-Il (Department of Veterinary Physiology, College of Veterinary Medicine, Gyeongsang National University) ;
  • Ma, Jin Yeul (Korean Medicine (KM)-Application Center, Korea Institute of Oriental Medicine (KIOM))
  • 투고 : 2021.05.12
  • 심사 : 2021.05.16
  • 발행 : 2021.05.31
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초록

Objectives : This study was conducted to evaluate the Intraocular pressure (IOP) and retina neuroprotective effect of Acer palmatum thumb. extract in glaucoma of DBA2/J mice vivo model. Methods : The amount of change in intraocular pressure(IOP) was measured every two weeks for 30 weeks in the DBA2/J glaucoma animal model. The increased IOP were regrouping into drug treatment groups. EW and EE (1, 2 mg/㎖)extract was eye drop administered twice per day for 8 weeks. After administration of the extract, IOP was measured every 1 week at a fixed time for 8 weeks, and protein expression levels and serological changes related to retinal function were investigated. Results : The DBA2/J mice were IOP increased as time dependent, resulting in damage to the retinal function protein related in glaucoma animal model. However, administration of extracts from Leaves of Acer palmatum thumb. prevented an increase IOP, increased for Brn3α, PKCα, and OPN1SW. Additionally, the experssion of Aterocyte maker, GFAP was decreased in DBA2/J mice. Conclusions : These results demonstrate that both IOP and age can be considered in studies using DBA2/J mice and suggest that the extracts from Leaves of Acer palmatum thumb. regulating of IOP and expression of proteins related to retinal function.

키워드

과제정보

본 연구는 한국한의학연구원 주요사업 임상수요기반치료기술개발사업 (과제고유번호 KSN2021230)의 지원 의해 한국한의학연구원에서 이루어졌으며, 이에 감사드립니다.

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