대한한의학방제학회지 (Herbal Formula Science)

  • 제29권2호
  • /
  • Pages.81-91
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  • 2021
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  • 1229-1218(pISSN)
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  • 2288-5641(eISSN)
대한한의학방제학회 (The Korean Medicine Society for the Herbal Formula Study)
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홍삼·백년초 복합물이 관절염 개선에 미치는 효과

Effects of red ginseng and Baeknyeoncho complex on the arthritis improvement

  • 이승현 (우석대학교 식품생명공학과) ;
  • 이창현 (우석대학교 한의과대학) ;
  • 김홍준 (우석대학교 한의과대학) ;
  • 김영식 (우석대학교 한의과대학) ;
  • 신민지 (우석대학교 식품생명공학과) ;
  • 김지현 (우석대학교 식품생명공학과) ;
  • 마상용 (우석대학교 식품생명공학과) ;
  • 이세연 (우석대학교 한약학과) ;
  • 권진 (한국복지대학 의료보장구과) ;
  • 오찬호 (우석대학교 식품생명공학과)
  • Lee, Seung-Hyeon (Department of Food & Biotechnology, Woosuk University) ;
  • Lee, Chang-Hyun (College of Korean Medicine, Woosuk University) ;
  • Kim, Hong-Jun (College of Korean Medicine, Woosuk University) ;
  • Kim, Young-Sik (College of Korean Medicine, Woosuk University) ;
  • Shin, Min-Ji (Department of Food & Biotechnology, Woosuk University) ;
  • Kim, Ji-Hyun (Department of Food & Biotechnology, Woosuk University) ;
  • Ma, Sang-Yong (Department of Food & Biotechnology, Woosuk University) ;
  • Lee, Se-Youn (Department of Korean Pharmacy, Woosuk University) ;
  • Kwon, Jin (Department of Prosthetics and Orthotics, Korean National College of Welfare) ;
  • Oh, Chan-Ho (Department of Food & Biotechnology, Woosuk University)
  • 투고 : 2021.04.01
  • 심사 : 2021.04.09
  • 발행 : 2021.05.31
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초록

Objectives : This study was conducted to evaluate the efficacy of a complex mixture of natural substances of ginseng and baeknyeoncho on the arthritic rats. Methods : In vitro experiments were conducted to ensure the stability of the complex. After setting toxicity and concentration by MTT assay, the antioxidant effect was measured through DPPH and ABTS radical scavenging activity. To confirm the anti-inflammatory effects of the complex, levels of nitric oxide (NO) and pro-inflammatory cytokines (IL-1β, TNF-α) were measured in LPS-treated macrophage cell lines (RAW264.7). We injected monosodium iodoacetate (MIA) 50 μl (60 mg/ml) into knee joints of rats to induce osteoarthritis. The rats were divided into three groups (normal (n=5), control (n=5), and OR (n=5) group). The control group consumed 2 mg/kg of physiological saline once a day for 4 weeks, and the OR group was mixed at a concentration of 416.5 mg/kg of Baengnyeoncho (O) and 208.25 mg/kg of red ginseng (R) and ingested 1 mL each 5 days a week. Results : This complex increased the DPPH and ABTS radical scavenging rate. The complex decreased NO production and pro-inflammatory cytokine production of macrophages. In the OR group, the secretion of cytokine in serum was decreased. In histopathological examination, the joint tissue of the composite showed less damage to the synovial membrane, cartilage, and fibrous tissue than the control group. Conclusions : As a result of this study, natural complexes have antioxidant, anti-inflammatory and cartilage protection effects. Therefore, we expect the complex to be effective in treating osteoarthritis.

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참고문헌

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