Herbal Formula Science (대한한의학방제학회지)

The Korean Medicine Society for the Herbal Formula Study (대한한의학방제학회)
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Protective Effects of the Calyx of Diospyros kaki Thunb. on H2O2-induced Oxidative Stress in C2C12 Myoblasts

C2C12 근육세포에서 과산화수소로 유도된 산화 스트레스에 대한 시체(柿蒂)의 보호효과

  • Park, Jeong Hwan (KM Data Division, Korea Institute of Oriental Medicine) ;
  • Lim, Jinyeong (Department of Food Science and Biotechnology, Chungbuk National University) ;
  • Lee, Mina (Department of Food Science and Biotechnology, Chungbuk National University) ;
  • Seong, Eunjeong (Department of Food Science and Biotechnology, Chungbuk National University) ;
  • Lee, Junsoo (Department of Food Science and Biotechnology, Chungbuk National University)
  • 박정환 (한국한의학연구원 한의약데이터부) ;
  • 임진영 (충북대학교 식품생명공학과) ;
  • 이민아 (충북대학교 식품생명공학과) ;
  • 성은정 (충북대학교 식품생명공학과) ;
  • 이준수 (충북대학교 식품생명공학과)
  • Received : 2021.09.10
  • Accepted : 2021.11.15
  • Published : 2021.11.30
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Abstract

Objectives : The calyx of Diospyros kaki Thunb. has usually been used to treat obstinate hiccup. This study investigated the protective effects of Diospyros kaki using C2C12 myoblasts of H2O2-induced oxidative stress. Methods : Cell viability and cytotoxicity were determined by MTT assay. The level of reactive oxygen species (ROS), malondialdehyde (MDA), and reduced glutathione (GSH) were measured by using optical properties. Results : The calyx of Diospyros kaki Thunb. extract showed no toxicity to C2C12 myoblasts until 20 ㎍/mL concentration and increased cell viability compared to the H2O2 treated group. The calyx of Diospyros kaki Thunb. extract inhibited the production of ROS and MDA at all concentrations. In addition, the calyx of Diospyros kaki Thunb. extract increased the concentration of GSH. Conclusion : This study provides that the calyx of Diospyros kaki Thunb. can be used as a potential material that exhibit antioxidative and protective effects on H2O2-induced oxidative stress in C2C12 Myoblasts.

Keywords

References

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