Journal of Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
권호 표지
DOI QR코드

DOI QR Code

Identifying antibacterial activity components of cosmos flower extracts

코스모스 추출물의 항균활성 성분 탐색

  • Kim, Mijung (Department of Health and Cosmetics, Dongduk Women's University) ;
  • Ahn, Seunghyun (Department of Applied Chemistry, Dongduk Women's University) ;
  • Park, Seyeon (Department of Applied Chemistry, Dongduk Women's University)
  • Received : 2020.07.20
  • Accepted : 2020.09.03
  • Published : 2020.09.30
Download PDF
⟨ Previous Next ⟩

Abstract

This study investigated whether the extracts from cosmos flowers exhibit antibacterial activities and identified which components were ascribed to the antibacterial effects. The antibacterial effects of extracts from white, pink, and violet cosmos flowers were observed for 24 h after inoculation with four kinds of bacteria, including Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa, and Escherichia coli. Among the three fractions of cosmos flower extracts, the best antibacterial activity against the four bacteria was observed in the extracts isolated from the EtOAc layer. However, the extracts from the CHCl3 layer were also effective against S. aureus. Moreover, the first of white, second of pink, and first of violet silica gel fractions (Fr.) isolated from the EtOAc layer exhibited minimal inhibition at a concentration of 0.1 mg/mL. Comparison of NMR and High-Pressure Liquid Chromatography results between silica gel Fr. and apigenin suggested that the effective fractions can contain a component including apigenin moiety.

본 연구는 코스모스꽃 추출물이 박테리아에 대해서 항균 활성이 있는지를 알아보고 이를 통해 항균 효과를 내는 물질에 대해 규명하고자 하였다. 흰색, 분홍색, 자주색의 코스모스꽃이 4종의 박테리아인 S. aureus, S. epidermidis, P. aeruginosa, E. coli에 대해 항균 효과가 있는지 접종 후 24 h 동안 관찰하였다. 3종의 꽃 추출물 중에 EtOAc층 분획물에서 4개의 박테리아에 대해 가장 우수한 항균 활성이 관찰되었고 S. aureus에 대해서는 CHCl3층 분획물도 효과를 보였다. 추가적으로 흰색 코스모스꽃 silica gel Fr. 1과 분홍색 코스모스꽃 silica gel Fr. 2, 자주색 코스모스꽃 silica gel Fr. 1은 0.1 mg/mL에서 최소저해농도가 나타났다. 이 분획물들과 코스모스 내에 존재할 것으로 유추되는 apigenin 간의NMR과 HPLC 비교, 분석한 결과를 통해서 유효 성분내에는 apigenin구조 요소가 함유되어 있는 물질이 있을 것으로 판단되었다.

Keywords

References

  1. Dubey S, Singh VK (2011) Population dynamics of Aphis spiracecola Patch (Homoptera: Aphididae) on medicinal plant Cosmos bipinnatus in eastern Uttar Pradesh, India. Adv Life Sci 1: 54-58. doi: 10.5923/j.als.20110102.10
  2. Edward FG, Teresa H (1999) Salvia splendens. Institute of Food and Agriculture Sciences, Dissertation, University of Florida
  3. Alexandros SB (2007) Plants used traditionally to treat malaria in Brazil. J Ethnobiol Ethnomed 3:18, doi:10.1186/1746-4269-3-18
  4. Molder M, Owens JN (1985) Cosmos. CRC handbook of flowering. CRC, Boca Raton
  5. Woo JH, Shin SL, Lee CH (2010) Antioxidant effects of ethanol extracts from flower species of compositae plant. J Korean Soc Food Sci Nutr 39: 159-164, doi: 10.3746/jkfn.2010.39.2.15
  6. Woo J, Shin S, Chang Y, Lee C (2010) Antioxidant effect according to extraction method in extracts of Dendranthema zawadskii var. yezoense and Cosmos bipinnatus. Kor J Hort Sci Technol 28: 462-468
  7. Lee YG, Lee J, Lee NY, Kim NK, Jung DW, Wang W, Kim Y, Kim HG, Nguyen TN, Park H, Baek NI (2017) Evaluation for the flowers of compositae plants as whitening cosmetics functionality. J Appl Biol Chem 60: 511. doi: 103839/jabc.2017.002
  8. Shin YH (2020) A Study on Antioxidant and Antimicrobial Activities of Cosmos bipinnatus Flower Extracts for Cosmeceutical Formulations. Dissertation, University of Hannam
  9. Woo WS (2014) Natural chemical research method. Seoul University Publishing Center, Seoul, pp 63-64
  10. Friend J (1979) Phenolic substances and plant disease. In: Swain T, Harborne JB, Van Sunere CF (eds) Biochemistry of Plant Phenolic, Plenum Press, New York, pp 557-588
  11. Friend J (1976) Lignification in infected tissue. In: Friend J, Threlfall DR (eds) Biochemical Aspects of Plant-Parasite Relationships, Academic Press, London
  12. Jang (2013) Radical Scavenging and Tyrosinase Inhibitory Effects of the Extracts and Fractions of Asteraceae Medicinal Plants. Dissertation, University of Sunchon
  13. Jang IC, Park JH, Park E, Park HR, Lee SC (2008) Antioxidative and antigenotoxic activity of extracts from cosmos (Cosmos bipinnatus) flowers. Plant foods Hum Nutr 63: 205-210, doi: 10.1007/s11130-008-0086-8
  14. Akihisa T, Yasukawa K, Oinuma H, Kasahara Y, Yamanouchi S, Takido M, Kumaki K, Tamura T (1996) Triterpene alcohols from the flowers of compositae and their anti-inflammatory effects. Phytochem 43: 1255-1260, doi:10.1016/S0031-9422(96)00343-3
  15. Kim SY, Lee MH, Park SN (2010) Evaluations of antioxidative activity and whitening effect of extracts from different parts of Cosmos bipinnatus. J of the Korean Oil Chemists' Soc 27: 559-567
  16. Olajuyigbe O, Ashafa A (2014) Chemical composition and antibacterial activity of essential oil of Cosmos bipinnatus Cav. leaves from South Africa. Iran J Pharm Res 13: 1417-1423, doi 10.22037/ijpr.2014.1581
  17. Lee SE, Kim S (2009) Composition and cytotoxicity of Essential Oil Extracted from Cosmos bipinnatus Cav. Weed Turf Sci (Conference) 29: 70-72
  18. Shibamoto T (1987) Retension indices in essential oil analysis. In: Sandra P, Bicchi C (eds.) Capillary gas chromatography in essential oils analysis, Huethig, Heidelberg, pp 259-274
  19. Saito K (1979) Quantitative variation of flavonoids and related compounds in Cosmos bipinnatus. Acta Soc Bot Pol 48: 317-325, doi:10.5586/asbp.2011.020
  20. Telange DR, Patil AT, Pethe AM, Fegade H, Anand S, Dave VS (2017) Formulation and characterization of an apigenin-phospholipid phytosome (APLC) for improved solubility, in vivo bioavailability, and antioxidant potential. Eur J Pharm Sci 108: 36-49, doi.org/10.1016/j.ejps.2016.12.009