Parasites, Hosts and Diseases

The Korea Society for Parasitology and Tropical Medicine (대한기생충학·열대의학회)
권호 표지
DOI QR코드

DOI QR Code

Development and Clinical Evaluation of a Rapid Diagnostic Test for Yellow Fever Non-Structural Protein 1

  • Received : 2019.02.27
  • Accepted : 2019.06.16
  • Published : 2019.06.30
Download PDF
⟨ Previous Next ⟩

Abstract

A rapid diagnostic test (RDT) kit was developed to detect non-structural protein 1 (NS1) of yellow fever virus (YFV) using monoclonal antibody. NS1 protein was purified from the cultured YFV and used to immunize mice. Monoclonal antibody to NS1 was selected and conjugated with colloidal gold to produce the YFV NS1 RDT kit. The YFV RDTs were evaluated for sensitivity and specificity using positive and negative samples of monkeys from Brazil and negative human blood samples from Korea. Among monoclonal antibodies, clones 3A11 and 3B7 proved most sensitive, and used for YFV RDT kit. Diagnostic accuracy of YFV RDT was fairly high; Sensitivity was 0.0% and specificity was 100% against Dengue viruses type 2 and 3, Zika, Chikungunya and Mayaro viruses. This YFV RDT kit could be employed as a test of choice for point-of-care diagnosis and large scale surveys of YFV infection under clinical or field conditions in endemic areas and on the globe.

Keywords

References

  1. Jentes ES, Poumerol G, Gershman MD, Hill DR, Lemarchand J, Lewis RF, Staples JE, Tomori O, Wilder-Smith A, Monath TP. The revised global yellow fever risk map and recommendations for vaccination, 2010: consensus of the Informal WHO Working Group on Geographic Risk for Yellow Fever. The Lancet Infect Dis 2011; 11: 622-632. https://doi.org/10.1016/S1473-3099(11)70147-5
  2. Tomlinson W, Hodgson RS. Centennial year of yellow fever eradication in New Orleans and the United States, 1905-2005. J La State Med Soc 2005; 157: 216-217.
  3. Kubota RL, de Brito M, Voltolini JC. Sweeping method to scan breeding places for dengue and urban yellow fever vetors. Rev Saude Publica 2003; 37: 263-265 (in Portuguese). https://doi.org/10.1590/S0034-89102003000200017
  4. Gubler D. The changing epidemiology of yellow fever and dengue, 1900 to 2003: full circle? Comp Immunol and Microbiol of Infect Dis 2004; 27: 319-330. https://doi.org/10.1016/j.cimid.2004.03.013
  5. World Health Organization. Yellow fever (Updated March 2016) [Internet]; Available from: http://www.who.int/mediacentre/factsheets/fs100/en/. [cited 2016 Mar 24].
  6. Morlais, Severson DW. Intraspecific DNA variation in nuclear genes of mosquito Aedes aegypti. Insect Mol Biol 2003; 12: 631-639. https://doi.org/10.1046/j.1365-2583.2003.00449.x
  7. Ravel S, Herve JP, Diarrassouba S, Kone A, Cuny G. Microsatellite markers for population genetic studies in Aedes aegypti (Diptera: Culicidae) from Cote d'Ivoire: evidence for a microgeographic genetic differentiation of mosquitoes from Bouake. Acta Trop 2002; 82: 39-49. https://doi.org/10.1016/S0001-706X(02)00028-1
  8. Vasconcelos PF, Monath TP. Yellow Fever Remains a Potential Threat to Public Health. Vector Borne Zoonotic Dis 2016; 16: 566-567. https://doi.org/10.1089/vbz.2016.2031
  9. Garske T, Van Kerkhove MD, Yactayo S, Ronveaux O, Lewis RF, Staples JE, Perea W, Ferguson NM. Yellow Fever in Africa: Estimating the Burden of Disease and Impact of Mass Vaccination from Outbreak and Serological Data. PLoS Med 2014; 11: e1001638. https://doi.org/10.1371/journal.pmed.1001638
  10. Woodall JP, Yuill TM. Why is the yellow fever outbreak in Angola a 'threat to the entire world'? Int J Infect Dis 2016; 48: 96-97. https://doi.org/10.1016/j.ijid.2016.05.001
  11. Ministerio da Saude Secretaria de Vigilancia em Saude. COES - Febre Amarela. INFORME - $N^{\circ}$ 30/2017 [Internet]; Available from: http://portalarquivos2.saude.gov.br/images/pdf/2017/marco/14/COES-FEBRE-AMARELA-INFORME-30.pdf.
  12. Muller DA, Young PR. The flavivirus NS1 protein: Molecular and structural biology, immunology, role in pathogenesis and application as a diagnostic biomarker. Antiviral Res 2013; 98: 192-208. https://doi.org/10.1016/j.antiviral.2013.03.008
  13. McBride WJ. Evaluation of dengue NS1 test kits for the diagnosis of dengue fever. Diagn Microbiol Infect Dis 2009; 64: 31-36. https://doi.org/10.1016/j.diagmicrobio.2009.01.002
  14. Richman DD, Cleveland PH, Redfield DC, Oxman MN, Wahl GM. Rapid viral diagnosis. Infect Dis 1984; 149: 298-310. https://doi.org/10.1093/infdis/149.3.298
  15. Chernesky MA, Mahony JB. Detection of viral antigens, particles, and early antibodies in diagnosis. Yale J Biol Med 1984; 57: 757-776.
  16. Lee J, Kim HY, Chong CK, Song HO. Development and clinical evaluation of a highly accurate dengue NS1 rapid test: from the preparation of a soluble NS1 antigen to the construction of an RDT. Diagn Microbiol Infect Dis 2015; 82: 128-134. https://doi.org/10.1016/j.diagmicrobio.2015.03.009
  17. Kim YH, Lee J, Kim YE, Chong CK, Pinchemel Y, Reisdorfer F, Coelho JB, Dias RF, Bae PK, Gusmao ZPM, Ahn HJ, Nam HW. Development of a rapid diagnostic test kit to detect IgG/IgM antibody against Zika virus using monoclonal antibodies to the envelope and non-structural protein 1 of the virus. Korean J Parasitol 2018; 56: 61-70. https://doi.org/10.3347/kjp.2018.56.1.61
  18. Friquet B, Chaffotte AF, Djavadi-Ohaniance L, Goldberg ME. Measurements of the true affinity constant in solution of antigen-antibody complexes by enzyme-linked immunosorbent assay. J Immunol Methods 1985; 77: 305-319. https://doi.org/10.1016/0022-1759(85)90044-4
  19. Chong CK, Jeong W, Kim HY, An DJ, Jeoung HY, Ryu JE, Ko AR, Kim YJ, Hong SJ, Yang Z, Nam HW. Development and clinical evaluation of a rapid serodiagnostic test for toxoplasmosis of cats using recombinant SAG1 antigen. Korean J Parasitol 2011; 49: 207-212. https://doi.org/10.3347/kjp.2011.49.3.207
  20. Alcon S, Talarmin A, Debruyne M, Falconar A, Deubel V, Flamand M. Enzyme-linked immunosorbent assay specific to Dengue virus type 1 nonstructural protein NS1 reveals circulation of the antigen in the blood during the acute phase of disease in patients experiencing primary or secondary infections. J Clin Microbiol 2002; 40: 376-381. https://doi.org/10.1128/JCM.40.02.376-381.2002
  21. Watterson D, Modhiran N, Young PR. The many faces of the flavivirus NS1 protein offer a multitude of options for inhibitor design. Antiviral Res 2016; 130: 7-18. https://doi.org/10.1016/j.antiviral.2016.02.014
  22. Alcala AC, Medina F, Gonzalez-Robles A, Salazar-Villatoro L, Fragoso-Soriano RJ, Vasquez C, Cervantes-Salazar M, del Angel RM, Ludert JE. The dengue virus non-structural protein 1 (NS1) is secreted efficiently from infected mosquito cells. Virology 2016; 488: 278-287. https://doi.org/10.1016/j.virol.2015.11.020