Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Understanding of Intrauterine Environment Changes based on Proteomics and Bioinformatics during Estrous Cycle

단백체학과 생물정보학을 이용한 자궁 내 환경의 이해

  • Lee, Sang-Hee (Discipline of ICT, School of Technology, Environments and Design, University of Tasmania) ;
  • Lee, Seunghyung (College of Animal Life Sciences, Kangwon National University)
  • 이상희 (타즈매니아대학교 기술환경디자인대학 ICT학과) ;
  • 이승형 (강원대학교 동물생명과학대학)
  • Received : 2019.04.30
  • Accepted : 2019.05.21
  • Published : 2019.05.30
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Abstract

Fertilization is the beginning of a new life that occurs in the female uterine. The female reproductive tract is composed ovary, oviduct, uterine, vagina and cervix, their physiological features are regulated by estrous cycle. Of these, uterine is a main point to establish embryo development and implantation, and intercommunication between embryo and uterine environment is necessary for suitable pregnancy. Endometrium is part of the uterine, its morphology is repetitively changed by hormones, and characteristic of uterine fluid from endometrium is also changed. Recently, massive proteins of endometrium and uterine fluid can be detected according to develop proteomics and bioinformatics and have been accelerated the understanding of the reproductive biology fields. Moreover, the massive protein information is actively studying with deeply studied theory such as sex hormone signal pathway and angiogenesis in mammals. In this paper, we review understanding of endometrium remodeling, uterine gland and fluid during estrous cycle, additionally studies on endometrium and uterine fluid based on proteomics techniques. Lastly, we introduced methods of the protein-protein correlation using bioinformatics tool that interaction with hormone receptors, representative angiogenetic factors and detected proteins using proteomics in endometrium and uterine fluid. This review will be useful to understanding the study on search of new cell mechanism in endometrium and uterine fluid.

암컷의 자궁에서 일어나는 수정은 새로운 생명의 시작점이다. 암컷의 번식기관은 난소, 난관, 자궁, 자궁경부 및 질로 구성되어 있으며, 이들기관은 발정주기에 따라 생리학적인 역할이 조절된다. 자궁은 수정란의 발달과 착상이 이루어지는 곳이기 때문에, 수정란과 자궁 환경의 상호작용은 안정적인 임신을 위한 필수적인 조건으로 알려져있다. 자궁내막은 자궁의 한 부분으로써 이들의 형태학적인 특징은 호르몬에 의해 반복적으로 변화되며, 자궁내막으로부터 분비되는 자궁액 역시 그 특징이 변화하게 된다. 최근, 자궁내막 및 자궁액 내 포함된 대량의 단백질을 단백체학과 생물정보학의 발전에 따라 검출할 수 있게 되었으며, 이러한 기술에 의해 번식학 발전을 가속화하고 있다. 대량의 단백질 정보는 성호르몬 신호기전 및 혈관신생과 같은 이론 등을 깊게 연구할 수 있는 도구로써 이용되고 있다. 본 총설에서는 자궁내막의 재구성, 자궁선 및 자궁액에 대한 기초적인 생물학적인 지식을 바탕으로, 단백체학과 생물정보학을 활용한 자궁내막 및 자궁액 연구에 대해서 소개하고자 한다. 또한, 생물정보학 도구를 활용하여 단백체학에서 탐색된 자궁내막 및 자궁액 관련 단백질들의 상호작용 알아보는 방법에 대해서도 소개하였다. 따라서, 본 총설의 내용은 발정주기동안 자궁내막 안에서 일어나는 새로운 세포 신호기전을 탐색하는데 큰 도움이 될것이라 생각된다.

Keywords

SMGHBM_2019_v29n5_621_f0001.png 이미지

Fig. 1. Images of the ovary (A-D) and uterus (E-H) morphology during estrous cycle in sows.

SMGHBM_2019_v29n5_621_f0002.png 이미지

Fig. 2. Sixteen proteins interaction among progesterone receptor (PGR), estrogen receptor alpha (ESR1), myoglobin (MB), vascular endothelial grwoth factor D (VEGFD), vinculin (VCL), actin beta (ACTB), annexin A4 (ANXA4), haptoglobin (HP), metalloproteinase inhibitor (TIMP1), coatomer subunit gamma-2 (COPG2), alpha-enolase (ENO1), annexin A2 (ANXA2), VEGFA, VEGFR2 (KDR), angiopoetin 2 (ANGPT1) and Tie2, protein-protein interaction was STING database, protein interaction with only sixteen protein (a) and added network nodes based on STRING database (b), Red cycle indicate that paxillin (PXN), the protein was conneted between angiogenetic proteins (VEGFA, KDR) and cytoskeleton protein (VCL).

Table 1. Biological process and molecular funtion of estrous cycle-associated proteins

SMGHBM_2019_v29n5_621_t0001.png 이미지

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