Journal of Plant Biotechnology

  • 제46권4호
  • /
  • Pages.318-322
  • /
  • 2019
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  • 1229-2818(pISSN)
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  • 2384-1397(eISSN)
한국식물생명공학회 (The Korean Society of Plant Biotechnology)
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사과 종자 발아에 미치는 기계적 상처, 자엽제거 및 영양분의 영향

Effects of mechanical Injury, cotyledon elimination and nutrition for the germination of apple seeds (Malus pulmila var. domestica)

  • 최필선 (남부대학교 한방제약개발학과) ;
  • 이영진 (남부대학교 응급구조학과) ;
  • 유영법 (남부대학교 응급구조학과) ;
  • 최명석 (경상대학교 농업생명과학대학 환경산림과학부)
  • Choi, Pil Son (Department of Oriental Pharmaceutical Development, Nambu University) ;
  • Lee, Young Jin (Department of Emergency Medical Rescue, Nambu University) ;
  • Yu, Young Beob (Department of Emergency Medical Rescue, Nambu University) ;
  • Choi, Myung Suk (Division of Environmental Forest Science, Gyeongsang National University)
  • 투고 : 2019.10.11
  • 심사 : 2019.10.23
  • 발행 : 2019.12.31
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초록

유근과 자엽 부위에 기계적 상처를 준 사과종자의 발아율을 측정하였을 때 상처를 받지 않은 종자에 비하여 발아율이 증가되었으며, 상처부위와 밀접한 관계가 있는 것으로 나타났다. 또한 ¾자엽 부위를 갖는 배(¾CE), ½자엽 부위를 갖는 배(½CE), ¼자엽 부위를 갖는 배(¼CE) 그리고 자엽을 완전히 제거한 배(NCE)의 발아율은 정상적인 자엽을 갖는 배에 비해 감소하였으며, 감소된 발아율은 영양분으로서 MS용액과 적정 농도의 호르몬, 즉 GA3가 단독 혹은 혼용 첨가된 한천배지에서 증가되는 것으로 나타났다. 이러한 결과는 자엽 제거에 의해 사과의 발아율이 감소되며, 그 감소된 발아율은 GA3 (80 mg/L)와MS용액에 의해 상당히 회복됨을 보여주었다.

When dehulled apple seeds (Malus pilmila var. domestica) were induced to germinate, the rate of germination, which was increased by piercing both the radicle and cotyledon, was related to the site of injury. Also, the germination rates of an embryo with ¾ cotyledon (¾ CE), embryo with ½ cotyledon (½ CE), embryo with ¼ cotyledon (¼ CE), and embryo without cotyledon (NCE) gradually decreased compared to an embryo with a normal cotyledon, but it significantly recovered on the agar media containing MS solution and/or 80 mg/L gibberellin (GA3) as nutrition. The results show that the germination of the embryo was inhibited by the partial removal of cotyledon and also demonstrated that the rate of germination could be significantly increased by adding GA3 (80 mg/L) and/or MS medium.

키워드

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