Journal of Plant Biotechnology

  • 제46권4호
  • /
  • Pages.310-317
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  • 2019
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  • 1229-2818(pISSN)
  • /
  • 2384-1397(eISSN)
한국식물생명공학회 (The Korean Society of Plant Biotechnology)
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사과 '후지'의 잎 절편체로부터 신초 기관형성을 통한 식물체 재생에 적합한 배지조성

Optimal Medium Compositions for Plant Regeneration via Adventitious Shoot Formation Using 'Fuji' Apple Leaf Explants

  • 이윤경 (상명대학교 식물식품공학과) ;
  • 권영주 (상명대학교 식물식품공학과) ;
  • 형남인 (상명대학교 식물식품공학과)
  • Lee, Yoon Kyung (Department of Plant and Food Sciences, Sangmyung University) ;
  • ;
  • Hyung, Nam-In (Department of Plant and Food Sciences, Sangmyung University)
  • 투고 : 2019.10.01
  • 심사 : 2019.10.28
  • 발행 : 2019.12.31
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초록

사과 '후지'의 잎 절편체로부터 신초 기관형성을 통한 효율적인 식물체 재생 시스템을 확립하기 위하여 배지조성 중 식물생장조절제, 기본배지와 당의 종류 및 농도를 달리하여 실험을 실시하였다. 식물생장조절제 NAA가 첨가된 상태에서 기본배지 중 N6와 LS, cytokinin 중 BA와 TDZ를 비교하였을 때, N6 배지에 BA 5.0 mg/L를 사용하는 것이 신초 형성율과 절편체당 재생 신초수에서 효과적이었다. BA보다 신초 형성에 효과적인 것으로 알려진 TDZ는 본 실험에서는 전반적으로 신초 형성율이 낮았다. 탄소 공급원과 식물생장조절제의 조합 처리에서는 sorbitol 40 g/L의 처리가 신초 형성율 67.3%, 절편체당 신초수 4.3개로 가장 좋았고, 식물생장조절제는 BA 5.0 mg/L와 NAA 0.1 mg/L의 혼합처리가 가장 효과적이었다. 암조건에서 3주간 배양한 후 명조건으로 옮겨 총 8주간 배양하여 재생된 신초는 1/4MS에 IBA 0.2 mg/L가 첨가된 배지에서 발근을 유도한 후 활착시켜 온실에서 재배하였을 때 정상적인 표현형을 보여주었다.

Plant regeneration protocols for adventitious shoot organogenesis from apple (Malus domestica 'Fuji') leaf explants were developed in the present study. The effects of different basal media, types and concentrations of carbon sources, and concentrations of plant growth regulators were evaluated to determine the optimal shoot regeneration conditions for 'Fuji' apple leaf explants. On different treatments involving combinations of basal media, LS and N6 media, and different types and concentrations of cytokinins, 6-benzyl-adenine (BA) and thidiazuron (TDZ), shoot regeneration rates were the highest in the N6 medium combined with BA. Among the plant growth regulator and carbon source combination treatments, 5.0 mg/L BA, and 0.1 mg/L α-naphthalene acetic acid (NAA) with 40 g/L sorbitol was the optimal combination for shoot regeneration. In addition, the optimal sorbitol concentrations for shoot regeneration were 40 g/L and 60 g/L. The highest regeneration (81.8%) was achieved using 40 g/L sorbitol. The regenerated shoots elongated and rooted on rooting medium, consisting of 1/4 MS medium with 0.2 mg/L indole-3-butyric acid (IBA). The plantlets were acclimatized and the regenerated plants exhibited normal phenotypes.

키워드

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