Fig. 1. The effects of Ascidian shell extract on the growth of HaCaT cell line. Serum-starved HaCaT cells were incubated with Ascidian shell extract for 24 hours at the indicated concentration of Ascidian shell extract. The cell viability was estimated using WST-1 assay.
Fig. 2. The effects of Ascidian shell extract on the growth of B16F10 cell line. Serum-starved B16F10 cells were incubated with Ascidian shell extract for 24 hours at the indicated concentration of Ascidian shell extract. The cell viability was estimated using WST-1 assay.
Fig. 3. DPPH radical scavenging activity(%) of Ascidian shell extract.
Fig. 4. SOD-like activity (%) of Ascidian shell extract.
Fig. 5. Coagulation rate (%), and Hatching rate (%) of Ascidian shell extract.
Fig. 6. Arrhythmia test of Ascidian shell extract on zebrafish larvae. The heartbeat of zebrafish larvae after having been treated with Ascidian shell extract were counted.
Fig. 7. Protective effect of ASE against UVB-induced ROS generation in zebrafish. The embryos were exposed to UVB (50 mJ/㎠) and treated with ASE. After incubation, the embryos were stained with DCF-DA and intracellular ROS were detected by spectrofluorometry (A) and fluorescencemicroscopy (B).
Fig. 8. Protective effect of ASE against UVB-induced cell death in zebrafish. The embryos were exposed to UVB (50 mJ/㎠) and treated with ASE. After incubation, the embryos were stained with acridine orange and cell death was detected by spectrofluorometry (C) and fluorescence microscopy (D).
Fig. 9. Protective effect of ASE against LPS-induced ROS production in zebrafish. After incubation, the embryos were stained with DCF-DA and intracellular ROS were detected by spectrofluorometry (E) and fluorescence microscopy (F).
Fig. 10. Protective effect of ASE against LPS-induced cell death in zebrafish. After incubation, the embryos were stained with acridine orange and cell death were detected by spectrofluorometry (G) and fluorescence microscopy (H).
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