Journal of the Korean Applied Science and Technology (한국응용과학기술학회지)

The Korean Society of Applied Science and Technology (한국응용과학기술학회)
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Comparative study of the biological activities effect of Mentha arvensis L. extracts from water and 80% ethanol

열수 및 80% 에탄올로 추출한 박하의 생리활성 비교연구

  • Choi, Dong-Hun (Department of beauty healthcare, Graduate School, Daejeon University) ;
  • Seung, O-Tak (Department of Oriental Health Care, Joongbu University) ;
  • Lim, Mi-Hye (Department of beauty healthcare, Graduate School, Daejeon University)
  • 최동훈 (대전대학교 자연과학대학 뷰티건강관리학과 대학원) ;
  • 승오탁 (중부대학교 한방건강관리학과) ;
  • 임미혜 (대전대학교 자연과학대학 뷰티건강관리학과 대학원)
  • Received : 2019.03.05
  • Accepted : 2019.03.29
  • Published : 2019.03.31
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Abstract

The purpose of this study was to investigated the biological activities such as cytotoxic, anti-oxidant and anti-inflammatory using Mentha arvensis L. extracts. Mentha arvensis L. was prepared by extracting with DW and 80% ethanol, after cell viability was assessed by MTT assay using RAW 264.7 cells. Antioxidant activities, and Anti-inflammatory activities measured through changes in the levels of reactive oxygen species (ROS), nitric oxide (NO), leukotrien B4 (LTB4), and anti- or in-flammatory cytokines($IL-1{\beta}$, IL-6, tumor necrosis factor $(TNF)-{\alpha}$, and IL-10) on LPS-induced RAW 264.7 cells. All test measured by ELISA reader and Luminex. Mentha arvensis L. was no cytotoxic in water and 80% ethanol extracts, and Concentration of 100 ug/ml of 80% ethanol extract was suppressed the productions of ROS in LPS-induced RAW 264.7 cells. Also, Productions of NO, LTB4, inflammatory or anti-inflammatory cytokines showed efficacy change that dose-independent of all extracts. In other words, Mentha arvensis L. showed significant biological activities showing anti-inflammatory, and anti-oxidant. These results may be developed as a raw material for new health food and therapeutics to ease the symptoms mentioned above.

본 연구는 박하 추출물을 통해 독성검사 및 항산화, 항염증과 같은 생리활성을 평가하고자 하였다. 박하 추출은 열수 및 80% 에탄올로 추출하였으며, RAW 264.7 세포를 통해 MTT를 통해 세포 생존율을 평가하였다. 또한, LPS로 유도한 RAW 264.7 세포를 통해 활성산소(ROS), 산화질소(NO), 류코트리엔 B4($LTB_4$), 항염 또는 염증성 사이토카인($IL-1{\beta}$, IL-6, $TNF-{\alpha}$, IL-10) 등을 ELISA 및 Luminex로 측정하였다. 그 결과, 박하 추출물은 열수 및 80% 에탄올 추출물에서 세포독성이 없었으며, 80% 에탄올 추출물의 $100{\mu}g/m{\ell}$ 농도에서 활성산소의 생성을 억제하였다. 또한, NO, LTB4, 염증 또는 항염증성 사이토카인의 생성을 모든 추출물에서 농도 의존적 효능을 나타냈다. 즉, 박하 추출물은 세포독성 없이 항산화 및 항염증 활성을 나타내는 생리학적 효능이 나타났다. 이와 같은 결과는 본 연구와 관련된 증상을 개선하기 위한 새로운 건강식품 및 치료제의 원료로 개발될 수 있다.

Keywords

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Fig. 1. Effect of Mentha arvensis L. extracts from DW and 80% EtOH on cell viability in RAW 264.7 cells.

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Fig. 2. Effect of Mentha arvensis L. extracts from DW and 80% EtOH on ROS levels in RAW 264.7 cells.

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Fig. 3. Effect of Mentha arvensis L. extracts from DW and 80% EtOH on NO levels in RAW 264.7 cells.

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Fig. 4. Effect of Mentha arvensis L. extracts from DW and 80% EtOH on LTB4 levels in RAW 264.7 cells.

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Fig. 5. Effect of Mentha arvensis L. extracts from DW and 80% EtOH on cytokine IL-1β (A), IL-6 (B), TNF-α (C), and IL-10 (D) levels in RAW 264.7 cells.

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