Biomaterials and Biomechanics in Bioengineering

  • 제4권1호
  • /
  • Pages.1-8
  • /
  • 2019
  • /
  • 2465-9835(pISSN)
  • /
  • 2465-9959(eISSN)
테크노프레스 (Techno-Press)
권호 표지
DOI QR코드

DOI QR Code

Effect of polymer concentration in cryogelation of gelatin and poly (vinyl alcohol) scaffolds

  • Ceylan, Seda (Department of Chemical Engineering, Mersin University) ;
  • Demir, Didem (Department of Chemical Engineering, Mersin University) ;
  • Gul, Gulsah (Department of Chemical Engineering, Mersin University) ;
  • Bolgen, Nimet (Department of Chemical Engineering, Mersin University)
  • 투고 : 2018.03.09
  • 심사 : 2018.09.07
  • 발행 : 2019.03.25
⟨ 이전 논문 다음 논문 ⟩

초록

The aim of this study was to investigate the effect of total polymer concentration on the chemical structure, morphology of pores, porosity, swelling ratio, degradation of gelatin-poly (vinyl alcohol) (Gel-PVA) cryogel scaffolds. Porous cryogels were prepared with cryogelation technique by using glutaraldehyde as a crosslinker. Functional group composition of cryogels after crosslinking was investigated by Fourier Transform Infrared (FTIR). The morphology of cryogels was characterized via scanning electron microscopy (SEM) and porosity analysis. All of the cryogels had a porous structure with an average pore size between $45.58{\pm}14.28$ and $50.14{\pm}4.26{\mu}m$. The cryogels were biodegradable and started to degrade in 14 days. As the polymer concentration increased the swelling ratio, the porosity and the degradation rate decreased. Spongy and mechanically stable Gel-PVA cryogels, with tunable properties, can be potential candidates as scaffolds for tissue engineering applications.

키워드

과제정보

연구 과제 주관 기관 : Mersin University

참고문헌

  1. Alhosseini, S.N., Moztarzadeh, F., Mozafari, M., Asgari, S., Dodel, M., Samadikuchaksaraei, A., Kargozar, S. and Jalali, N. (2012), "Synthesis and characterization of electrospun polyvinyl alcohol nanofibrous scaffolds modified by blending with chitosan for neural tissue engineering", J. Nanomedicine, 7, 25-34.
  2. Annabi, N., Nichol, J.W., Zhong, X., Ji, C., Koshy, S., Khademhosseini, A. and Dehghani, F. (2010), "Controlling the porosity and microarchitecture of hydrogels for tissue engineering", Tissue Eng. Part B Rev., 16(4), 371-383. https://doi.org/10.1089/ten.teb.2009.0639
  3. Burg, K.J.L. and Orr, D.E. (2008), "An overview of bioresorbable materials", Degrad. Rate Bioresorbable Mater. Predict. Eval., 1, 3-18. https://doi.org/10.1533/9781845695033.1.3
  4. Ceylan, S., Gokturk, D. and Bolgen, N. (2016), "Effect of crosslinking methods on the structure and biocompatibility of polyvinyl alcohol/gelatin cryogels", Biomed. Mater. Eng., 27(4), 327-340.
  5. Ceylan, S., Gokturk, D., Demir, D., Damla Ozdemir, M. and Bolgen, N. (2017), "Comparison of additive effects on the PVA/starch cryogels: Synthesis, characterization, cytotoxicity, and genotoxicity studies", J. Polym. Mater. Polym. Biomater., 67(14), 855-864.
  6. Choi, S.M., Singh, D., Kumar, A., Oh, T.H., Cho, Y.W. and Han, S.S. (2013), "Porous three-dimensional PVA/gelatin sponge for skin tissue engineering", J. Polym. Mater. Polym. Biomater., 62(7), 384-389. https://doi.org/10.1080/00914037.2012.710862
  7. Choudhury, M., Mohanty, S. and Nayak, S. (2015), "Effect of different solvents in solvent casting of porous PLA scaffolds: In biomedical and tissue engineering applications", J. Biomater. Tissue Eng., 5(1), 1-9. https://doi.org/10.1166/jbt.2015.1243
  8. Dispinar, T., Van Camp, W., De Cock, L.J., De Geest, B.G. and Du Prez, F.E. (2012), "Redox-Responsive Degradable PEG Cryogels as Potential Cell Scaffolds in Tissue Engineering", Macromol. Biosci., 12(3), 383-394. https://doi.org/10.1002/mabi.201100396
  9. Gualandi, C. (2011), Porous Polymeric Bioresorbable Scaffolds for Tissue Engineering, Springer Science and Business Media, Berlin, Germany.
  10. Imani, R., Rafienia, M. and Hojjati Emami, S. (2013), "Synthesis and characterization of glutaraldehydebased crosslinked gelatin as a local hemostat sponge in surgery: An in vitro study", Biomed. Mater. Eng., 23(3), 211-224.
  11. Kemence, N. and Bolgen, N. (2017), "Gelatin-and hydroxyapatite-based cryogels for bone tissue engineering: synthesis, characterization, in vitro and in vivo biocompatibility", J. Tissue Eng. Regen. Med., 11(1), 20-33. https://doi.org/10.1002/term.1813
  12. Mansur, H.S., Sadahira, C.M., Souza, A.N. and Mansur, A.A P. (2008), "FTIR spectroscopy characterization of poly (vinyl alcohol) hydrogel with different hydrolysis degree and chemically crosslinked with glutaraldehyde", Mater. Sci. Eng. C, 28(4), 539-548. https://doi.org/10.1016/j.msec.2007.10.088
  13. Nasri-Nasrabadi, B., Mehrasa, M., Rafienia, M., Bonakdar, S., Behzad, T. and Gavanji, S. (2014), "Porous starch/cellulose nanofibers composite prepared by salt leaching technique for tissue engineering", Carbohydr. Polym., 108(1), 232-238. https://doi.org/10.1016/j.carbpol.2014.02.075
  14. Nguyen, T.H. and Lee, B.T. (2010), "Fabrication and characterization of cross-linked gelatin electro-spun nano-fibers", J. Biomed. Sci. Eng., 3(12), 1117. https://doi.org/10.4236/jbise.2010.312145
  15. Odabas, S. (2016), "Collagen-carboxymethyl cellulose-tricalcium phosphate multi-lamellar cryogels for tissue engineering applications: Production and characterization", J. Bioact. Compat. Polym., 31(4), 411-422. https://doi.org/10.1177/0883911515627472
  16. Rapa, M., Grosu, E., Andreica, M. and Hetvary, M. (2014), "Journal of Environmental Research and Protection Polyvinyl alcohol and starch blends : properties and biodegradation behavior", J. Environ. Res. Prot., 11(1), 34-42.
  17. Rodrigues, S.C., Salgado, C.L., Sahu, A., Garcia, M.P., Fernandes, M.H. and Monteiro, F.J. (2013), "Preparation and characterization of collagen-nanohydroxyapatite biocomposite scaffolds by cryogelation method for bone tissue engineering applications", J. Biomed. Mater. Res. Part A, 101A(4), 1080-1094. https://doi.org/10.1002/jbm.a.34394
  18. Tocchio, A., Tamplenizza, M., Martello, F., Gerges, I., Rossi, E., Argentiere, S., Rodighiero, S., Zhao, W., Milani, P. and Lenardi, C. (2015), "Versatile fabrication of vascularizable scaffolds for large tissue engineering in bioreactor", Biomaterials, 45, 124-131. https://doi.org/10.1016/j.biomaterials.2014.12.031