The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Characterization of Six Novel Endophytic Fungi Isolated from Leaves of Plants Inhabiting Jeju Island

제주도에 서식하는 식물 잎에서 분리된 6종의 국내 미기록 내생균 보고

  • Park, Hyeok (Department of Biology Education, Korea National University of Education) ;
  • Choi, Young-Joon (Department of Biology, Kunsan National University) ;
  • Eom, Ahn-Heum (Department of Biology Education, Korea National University of Education)
  • 박혁 (한국교원대학교 생물교육과) ;
  • 최영준 (군산대학교 생물학과) ;
  • 엄안흠 (한국교원대학교 생물교육과)
  • Received : 2018.11.15
  • Accepted : 2018.11.19
  • Published : 2018.12.01
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Abstract

Endophytic fungi were isolated from the leaves of diverse plants inhabiting Jeju Island, Korea. The fungal isolates were identified through phylogenetic analyses incorporating nucleotide sequences derived from the internal transcribed spacer region, large subunit region of ribosomal DNA, and beta-tubulin gene. Our results identified six endophytic fungi previously unknown in Korea namely, Diaporthe goulteri, Diaporthe vaccini, Rhizosphaera pini, Valsa friesii, Xylaria primorskensis, and Zalerion arboricola were identified. Here, we present their cultural and morphological characteristics and phylogenetic relationship.

제주도에 서식하는 다양한 식물의 잎에서 내생균을 분리하였다. 분리된 균주들은 형태적 특성 및 internal transcribed spacer, large subunit rDNA 영역 및 beta-tubulin rDNA 유전자의 염기서열의 계통 분석을 통해 종을 동정하였다. 그 결과 6종의 국내 미기록 내생균을 확인하였고, 확인된 종은 각각 Diaporthe goulteri, Diaporthe vaccini, Rhizosphaera pini, Valsa friesii, Xylaria primorskensis, Zalerion arboricola 이다. 확인된 6종의 미기록 내생균 균주의 형태적 특성 및 염기서열 계통분석의 결과에 대해 기술하였다.

Keywords

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Fig. 1. Colonies of strain P8 (Diaporthe goulteri) grown for 7 days on PDA (A) and MEA (G), conidia (M). Colonies of strain 17E026 (Diaporthe vaccinii) grown for 7 days on PDA (B) and MEA (H). Colonies of strain 16H073 (Rhizosphaera pini) grown for 7 days on PDA (C) and MEA (I), conidiophore and conidium (N, O). Colonies of strain 16H342 (Valsa friesii) grown for 7 days on PDA (D) and MEA (J). Colonies of strain 17E107 (Xylaria primorskensis) grown for 7 days on PDA (E) and MEA (K), conidia (P). Colonies of strain 16H107 (Zalerion arboricola) grown for 7 days on PDA (F) and MEA (L), conidiophore and conidia (Q, R). PDA, potato dextrose agar; MEA, malt extract agar (scale bar = 10 μm).

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Fig. 2. Neighbor-joining phylogenetic tree based on a combined alignment of internal transcribed spacer, large subunit rDNA, and beta-tubulin rDNA sequences. Nectria dacryocarpa was used as an outgroup. Numbers on branches indicate bootstrap values (1,000 replicates). Bold letters indicates fungal strains isolated in this study.

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Fig. 3. Neighbor-joining phylogenetic tree based on a combined alignment of internal transcribed spacer and large subunit rDNA sequences. Phomopsis tuberivora was used as an outgroup. Numbers on branches indicate bootstrap values (1,000 replicates). Bold letters indicates fungal strains isolated in this study.

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Fig. 4. Neighbor-joining phylogenetic tree based on a combined alignment of internal transcribed spacer and large subunit rDNA sequences. Diaporthe acaciarum was used as an outgroup. Numbers on branches indicate bootstrap values (1,000 replicates. Bold letters indicates fungal strains isolated in this study.

GNHHDL_2018_v46n4_405_f0005.png 이미지

Fig. 5. Neighbor-joining phylogenetic tree based on a combined alignment of internal transcribed spacer and large subunit rDNA sequences. Botryosphaeria dothidea was used as an outgroup. Numbers on branches indicate bootstrap values (1,000 replicates). Bold letters indicates fungal strains isolated in this study.

GNHHDL_2018_v46n4_405_f0006.png 이미지

Fig. 6. Neighbor-joining phylogenetic tree based on a combined alignment of internal transcribed spacer and large subunit rDNA sequences. Glonium stellatum was used as an outgroup. Numbers on branches indicate bootstrap values (1,000 replicates). Bold letters indicates fungal strains isolated in this study.

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