Journal of the Korean Applied Science and Technology (한국응용과학기술학회지)

The Korean Society of Applied Science and Technology (한국응용과학기술학회)
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Anti-inflammatory Effect of Skate Collagen Peptide through Attenuation of Oxidative Stress

홍어 콜라겐 펩타이드의 산화적 스트레스 완화를 통한 항염증효과

  • Jeong, Kap Seop (Department of Food Science & Nutrition, Tongmyong University) ;
  • Noh, Jeong Sook (Department of Food Science & Nutrition, Tongmyong University)
  • 정갑섭 (동명대학교 식품영양학과) ;
  • 노정숙 (동명대학교 식품영양학과)
  • Received : 2018.11.26
  • Accepted : 2018.12.23
  • Published : 2018.12.31
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Abstract

The purpose of this study was to investigate the effect of skate skin collagen peptide (SCP) according to its molecular weight (<1,000 Da or >1,000 Da) which was divided using the ultrafilatration method. The 200 mg/kg collagen peptide was administrated to obesity-induced db/db mice for 8 weeks. As the results, in collagen peptide-treated groups, body weight gain was decreased, the plasma and hepatic concentration of reactive oxygen species decreased and oxidative stress was alleviated. In addition, SCP-treated group showed the significant reduction of the protein expressions of nuclear transcription factors($NF-{\kappa}B$), enzymes(COX2, iNOS), and inflammatory cytokine(IL-6) in hepatic tissue, compared with those of the obese control group. There was a slight difference depending on the molecular weight of collagen peptide, but overall it was not significant. Therefore, SCP effectively inhibited the obesity-induced inflammatory response through attenuation of oxidative stress in the liver.

홍어 껍질 유래 콜라겐 펩타이드를 한외여과막을 이용하여 분자량의 크기를 1,000 Da 이하와 1,000 Da 이상으로 분리하고 그 효과를 비교하고자 하였다. 각각의 시료를 비만 유발 실험동물인 db/db mice에 체중당 200 mg의 콜라겐 펩타이드를 8주간 투여하였다. 본 연구의 결과는 콜라겐 펩타이드를 투여한 군에서 비만대조군에 비해 체중 증가량의 감소, 혈액 및 간조직의 활성산소 농도의 감소, 간조직의 산화적 스트레스가 완화된 것으로 나타났다. 또한 콜라겐 투여군에서 염증반응과 관련된 간조직의 핵전사인자($NF-{\kappa}B$) 및 효소(COX2, iNOS), 염증성 사이토카인(IL-6)발현이 비만대조군에 비해 유의적으로 감소하였다. 분자량의 크기에 따라 약간의 차이가 나타났지만, 전반적으로는 유의적이지 않았다. 따라서 홍어 콜라겐 펩타이드의 비만에 의해 유발된 염증반응이 억제되었는데, 이는 콜라겐의 산화적 스트레스 완화작용에 기인한 것으로 사료된다.

Keywords

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Fig. 1. Effect of collagen peptide of skate skin on serum ROS and peroxynitrite levels.

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Fig. 2. Effect of collagen peptide of skate skin on oxidative stress-related factors in the hepatic tissue.

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Fig. 3. Effect of collagen peptide of skate skin on inflammatory factors in the hepatic tissue.

Table 1. Effect of skate collagen peptide on body weight gain and food efficiency ratio in obese db/db mice

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