Journal of Korean Society of Occupational and Environmental Hygiene (한국산업보건학회지)

Korean Industrial Hygiene Association (한국산업보건학회)
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Biological Monitoring of Workers Exposed to Diisocyanates using Urinary Diamines

소변 중 디아민을 이용한 디이소시아네이트 노출 근로자의 생물학적 모니터링

  • Lee, Jong Seong (Occupational Lung Diseases Research Institute, Korea Workers' Compensation & Welfare Service) ;
  • Kim, Boowook (Occupational Lung Diseases Research Institute, Korea Workers' Compensation & Welfare Service) ;
  • Shin, Jungah (Occupational Lung Diseases Research Institute, Korea Workers' Compensation & Welfare Service) ;
  • Baek, JinEe (Occupational Lung Diseases Research Institute, Korea Workers' Compensation & Welfare Service) ;
  • Shin, Jae Hoon (Occupational Lung Diseases Research Institute, Korea Workers' Compensation & Welfare Service) ;
  • Kim, Ji-hye (Occupational Lung Diseases Research Institute, Korea Workers' Compensation & Welfare Service)
  • 이종성 (근로복지공단 직업성폐질환연구소) ;
  • 김부욱 (근로복지공단 직업성폐질환연구소) ;
  • 신정아 (근로복지공단 직업성폐질환연구소) ;
  • 백진이 (근로복지공단 직업성폐질환연구소) ;
  • 신재훈 (근로복지공단 직업성폐질환연구소) ;
  • 김지혜 (근로복지공단 직업성폐질환연구소)
  • Received : 2016.05.04
  • Accepted : 2016.06.28
  • Published : 2016.06.30
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Abstract

Objectives: Diisocyanates are a potent inducer of diseases of the airways, especially asthma. In this study, toluenediamine(TDA) and methylenedianiline(MDA) in urine were evaluated as biomarkers of exposure to tolunenediisocyanate(TDI) and methylenediphenyl diisocyanate(MDI), respectively. Methods: Workers exposed to TDI and MDI, as well as non-occupationally exposed subjects, were studied and pre- and post-shift urine samples were collected from 8 control subjects and 8 workers from a factory which manufactures polyurethane products for reducing noise and vibration in automobiles. Airborne TDI and MDI(n=8) were sampled on solvent-free glass filters impregnated with n-butylamine and detected by liquid chromatography atmospheric pressure ionization tandem mass spectrometry. Urinary TDA and MDA were detected as pentafluoropropionic acid anhydride(PFPA) derivatives by liquid chromatography electrospray ionization tandem mass spectrometry. Results: The median levels of urinary 2,6-TDA(p<0.001), 2,4-TDA(p=0.001), and MDA(p<0.001) of workers in post-shift samples were significantly higher than those of controls. The median levels of urinary 2,6-0TDA($0.63{\mu}g/g$ creatinine vs $0.34{\mu}g/g$ creatinine, p=0.017) and MDA($4.21{\mu}g/g$ creatinine vs $3.18{\mu}g/g$ creatinine, p=0.017) of workers in post-shift samples were significantly higher than those of the pre-shift samples. There were significant correlations between the urinary 2,6-TDA, 2,4-TDA, and MDA of workers in post-shift samples and the airborne 2,6-TDI(rho=0.952, p<0.001), 2,4-TDI(rho=0.833, p=0.001), and MDI(rho=0.952, p<0.001). Conclusions: These urinary diamines, metabolites of diisocyanates, in post-shift samples were useful biomarkers to assess occupational exposure to diisocyanates.

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