Asian Pacific Journal of Cancer Prevention

  • 제17권4호
  • /
  • Pages.1891-1898
  • /
  • 2016
  • /
  • 1513-7368(pISSN)
  • /
  • 2476-762X(eISSN)
아시아태평양암예방학회 (Asian Pacific Journal of Cancer Prevention)
권호 표지
DOI QR코드

DOI QR Code

Anti-Cancer Effects of Imperata cylindrica Leaf Extract on Human Oral Squamous Carcinoma cell line SCC-9 in Vitro

  • 발행 : 2016.06.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Imperata cylindrica, a tall tufted grass which has multiple pharmacological applications is one of the key ingredients in various traditional medicinal formula used in India. Previous reports have shown that I. cylindrica plant extract inhibited cell proliferation and induced apoptosis in various cancer cell lines. To our knowledge, no studies have been published on the effect of I. cylindrica leaf extract on human oral cancers. The present study was undertaken in order to evaluate the anticancer properties of the leaf extract of I. cylindrica using an oral squamous cell carcinoma cell line SCC-9 as an in vitro model system. A methanol extract from dried leaves of I. cylindrica (ICL) was prepared by standard procedures. Effects of the ICL extract on the morphology of SCC-9 cells was visualized by microscopy. Cytotoxicity was determined by MTT assay. Effects of the ICL extract on colony forming ability of SCC-9 cells was evaluated using clonogenic assay. Cell cycle analysis was performed by flow cytometry and induction of apoptosis was determined by DNA fragmentation assay. The ICL extract treatment caused cytotoxicity and induced cell death in vitro in SCC-9 cells in a dose-dependent manner. This treatment also significantly reduced the clonogenic potential and inhibited cell proliferation by arresting the cell cycle in the G2/M phase. Furthermore, DNA fragmentation assays showed that the observed cell death was caused by apoptosis. This is the first report showing the anticancer activity of the methanol extracts from the leaves of I. cylindrica in human oral cancer cell line. Our data indicates that ICL extract could be considered as one of the lead compounds for the formulation of anticancer therapeutic agents to treat/manage human oral cancers. The natural abundance of I. cylindrica and its wide geographic distribution could render it one of the primary resource materials for preparation of anticancer therapeutic agents.

키워드

참고문헌

  1. Amin AR, Kucuk O, Khuri FR, Shin DM, (2009). Perspectives for cancer prevention with natural compounds. J Clin Oncol, 27, 2712-25. https://doi.org/10.1200/JCO.2008.20.6235
  2. Anantharaman D, Gheit T, Waterboer T, et al (2013). Human papillomavirus infections and upper aero-digestive tract cancers: the ARCAGE study. J Natl Cancer Inst, 105, 536-45. https://doi.org/10.1093/jnci/djt053
  3. Arora S, Tandon S (2015). DNA fragmentation and cell cycle arrest: a hallmark of apoptosis induced by Ruta graveolens in human colon cancer cells. Homeopathy, 104, 36-47. https://doi.org/10.1016/j.homp.2014.10.001
  4. Ayurvedic Pharmacopoeia of India, Part 1, Vol. V, First Edition, (2006). Ministry of health and family welfare, department of ayurveda, yoga and naturopathy, unani, siddha and homoeopathy (AYUSH), Government of India, New Delhi.
  5. Chatelain K, Phippen S, McCabe J, et al (2011). Cranberry and grape seed extracts inhibit the proliferative phenotype of oral squamous cell carcinomas. Evid Based Complement Alternat Med, 2011, 467691.
  6. Chen M, Huang J, Yang X, et al (2012). Serum starvation induced cell cycle synchronization facilitates human somatic cells reprogramming. PLoS One, 7, 28203. https://doi.org/10.1371/journal.pone.0028203
  7. de Camargo-Cancela M, Voti L, Guerra-Yi M, et al (2010). Oral cavity cancer in developed and in developing countries: population-based incidence. Head Neck, 32, 357-67.
  8. Dikshit R, Gupta PC, Ramasundarahettige C, et al (2012). Cancer mortality in India: a nationally representative survey. Lancet, 379, 1807-16. https://doi.org/10.1016/S0140-6736(12)60358-4
  9. Doan, D.D., Nguyen, N.H., Doan, H.K., Nguyen, T.L., Phan, T.S., van Dau, N., Grabe, M., Johansson R, Lindgren G, Stjernstrom NE, (1992). Studies on the individual and combined diuretic effects of four Vietnamese traditional herbal remedies (Zea mays, Imperata cylindrica, Plantago major and Orthosiphon stamineus). J Ethnopharmacol, 36, 225-31. https://doi.org/10.1016/0378-8741(92)90048-V
  10. Ferlay J SI, Ervik M, Dikshit R, et al (2013). GLOBOCAN 2012 v1.0, Cancer Incidence and Mortality Worldwide. [Online]. lyon, france: international agency for research on cancer.
  11. Forman D, Bray F, Brewster DH, et al (2014). Cancer incidence in five continents. Vol X [Online]. Lyon, France: IARC scientific publication, No 164.
  12. Franken NA, Rodermond HM, Stap J, et al (2006). Clonogenic assay of cells in vitro. Nat Protoc, 1, 2315-9. https://doi.org/10.1038/nprot.2006.339
  13. Fulda S (2010). Modulation of apoptosis by natural products for cancer therapy. Planta Med, 76, 1075-9. https://doi.org/10.1055/s-0030-1249961
  14. Guruprasad KP, Mascarenhas R, Gopinath PM, et al (2010). Studies on Brahma rasayana in male swiss albino mice: chromosomal aberrations and sperm abnormalities. J Ayurveda Integr Med, 1, 40-4. https://doi.org/10.4103/0975-9476.59826
  15. Harborne JB (1998). Phytochemical methods- a guide to modern techniques of plant analysis, springer science & business media.
  16. Hengartner MO (2000). The biochemistry of apoptosis. Nature, 407, 770-6. https://doi.org/10.1038/35037710
  17. IARC. (2012). Personal Habits and Indoor Combustions [Online]. Lyon, France: IARC.
  18. Jayalakshmi S, Patra A, Lal VK, Ghosh, A K (2010). Pharmacognostical standardization of roots of Imperata cylindrica Linn (Poaceae). J Pharm Sci Res, 2, 5.
  19. Katz D, Ito E, Lau KS, et al (2008). Increased efficiency for performing colony formation assays in 96-well plates: novel applications to combination therapies and high-throughput screening. Biotechniques, 44, ix-xiv. https://doi.org/10.2144/000112757
  20. King M, Chatelain K, Farris D, et al (2007). Oral squamous cell carcinoma proliferative phenotype is modulated by proanthocyanidins: a potential prevention and treatment alternative for oral cancer. BMC Complement Altern Med, 7, 22. https://doi.org/10.1186/1472-6882-7-22
  21. Krishna Rao SV, Mejia G, Roberts-Thomson K, et al (2013). Epidemiology of oral cancer in Asia in the past decade--an update (2000-2012). Asian Pac J Cancer Prev, 14, 5567-77. https://doi.org/10.7314/APJCP.2013.14.10.5567
  22. Kuete V, Krusche B, Youns M, et al (2011). Cytotoxicity of some Cameroonian spices and selected medicinal plant extracts. J Ethnopharmacol, 134, 803-12. https://doi.org/10.1016/j.jep.2011.01.035
  23. Kuete V, Sandjo LP, Wiench B, et al (2013). Cytotoxicity and modes of action of four Cameroonian dietary spices ethno-medically used to treat cancers: Echinops giganteus, Xylopia aethiopica, Imperata cylindrica and Piper capense. J Ethnopharmacol, 149, 245-53. https://doi.org/10.1016/j.jep.2013.06.029
  24. Mitra R, Devi BI, Gope ML, et al (2012). Sodium butyrate modulates pRb phosphorylation and induces cell death in human vestibular schwannomas in vitro. Indian J Exp Biol, 50, 19-27.
  25. More Y, D'Cruz AK (2013). Oral cancer: review of current management strategies. Natl Med J India, 26, 152-8.
  26. Mosmann T (1983). Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods, 65, 55-63. https://doi.org/10.1016/0022-1759(83)90303-4
  27. Nair SV, Hettihewa M, Rupasinghe HP (2014). Apoptotic and inhibitory effects on cell proliferation of hepatocellular carcinoma HepG2 cells by methanol leaf extract of Costus speciosus. Biomed Res Int, 2014, 637098.
  28. Newman DJ, Cragg GM (2007). Natural products as sources of new drugs over the last 25 years. J Nat Prod, 70, 461-77. https://doi.org/10.1021/np068054v
  29. Pagliacci MC, Smacchia M, Migliorati G, et al (1994). Growthinhibitory effects of the natural phyto-oestrogen genistein in MCF-7 human breast cancer cells. Eur J Cancer, 30, 1675-82. https://doi.org/10.1016/0959-8049(94)00262-4
  30. Parvathy NG, Padma R, Renjith V, Rahate KP, Saranya TS (2012). Phytochemical screening and anthelmintic activity of methanolic extract of Imperata cylindrica. Int J Pharm Pharmaceut Sci, 4, 3.
  31. Pinilla V, Luu B (1999). Isolation and partial characterization of immunostimulating polysaccharides from Imperata cylindrica. Planta Med, 65, 549-52. https://doi.org/10.1055/s-1999-14087
  32. Pragya T, Verma U, Singh R, Joshi PK, Rout OP, (2015). Rasayana' herbs used in ayurveda -a review. World J Pharmacy Pharmaceutical Sciences, 8.
  33. Qi F, Li A, Inagaki Y, et al (2010). Chinese herbal medicines as adjuvant treatment during chemo- or radio-therapy for cancer. Biosci Trends, 4, 297-307.
  34. Rafehi H, Orlowski C, Georgiadis GT, et al (2011). Clonogenic assay: adherent cells. J Vis Exp.
  35. Sankaranarayanan R, Ramadas K, Thomas G, et al (2005). Effect of screening on oral cancer mortality in Kerala, India: a cluster-randomised controlled trial. Lancet, 365, 1927-33. https://doi.org/10.1016/S0140-6736(05)66658-5
  36. Shah NT, Pandya TN, Sharma PP, Patel BR, Acharya R, (2012). Mootrala Karma of Kusha [Imperata cylindrica Beauv.] and Darbha [Desmostachya bipinnata Stapf.] - A comparative study. Ayu, 33, 387-90. https://doi.org/10.4103/0974-8520.108829
  37. Saraswathy M, Gong S (2013). Different strategies to overcome multidrug resistance in cancer. Biotechnol Adv, 31, 1397- 407. https://doi.org/10.1016/j.biotechadv.2013.06.004
  38. Sushama B, Nishteswar K (2014). Review of anti-oxidant herbal drugs w.s.r to madhuraskandha (charakasamhita). Int J Ayurvedic Herbal Med, 4, 1480-93.
  39. Thorat RV, Panse NS, Budukh AM, et al (2009). Prevalence of tobacco use and tobacco-dependent cancers in males in the rural cancer registry population at Barshi, India. Asian Pac J Cancer Prev, 10, 1167-70.
  40. Tsai YL, Chiu CC, Yi-Fu Chen J, et al (2012). Cytotoxic effects of Echinacea purpurea flower extracts and cichoric acid on human colon cancer cells through induction of apoptosis. J Ethnopharmacol, 143, 914-9. https://doi.org/10.1016/j.jep.2012.08.032
  41. Wyllie AH (1980). Glucocorticoid-induced thymocyte apoptosis is associated with endogenous endonuclease activation. Nature, 284, 555-6. https://doi.org/10.1038/284555a0
  42. Xavier CP, Lima CF, Preto A, et al (2009). Luteolin, quercetin and ursolic acid are potent inhibitors of proliferation and inducers of apoptosis in both KRAS and BRAF mutated human colorectal cancer cells. Cancer Lett, 281, 162-70. https://doi.org/10.1016/j.canlet.2009.02.041
  43. Yoon JS, Lee MK, Sung SH, et al (2006). Neuroprotective 2-(2-phenylethyl)chromones of Imperata cylindrica. J Nat Prod, 69, 290-1. https://doi.org/10.1021/np0503808

피인용 문헌

  1. Potential of Central, Eastern and Western Africa Medicinal Plants for Cancer Therapy: Spotlight on Resistant Cells and Molecular Targets vol.8, pp.1663-9812, 2017, https://doi.org/10.3389/fphar.2017.00343
  2. The Efficacy and Safety of a Herbal Toothpaste in Reducing Gingivitis: A Double-Blind, Randomized, Placebo-Controlled, Parallel Allocation Clinical Trial vol.2019, pp.1741-4288, 2019, https://doi.org/10.1155/2019/3764936