Journal of the Korean Applied Science and Technology (한국응용과학기술학회지)

The Korean Society of Applied Science and Technology (한국응용과학기술학회)
권호 표지
DOI QR코드

DOI QR Code

Physiological Activities using Root and Stem Extracts of Cymbidium

심비디움 뿌리 및 줄기 추출물의 생리 활성

  • Kim, Hye-Ran (Department of Clinical Laboratory Science, College of Health Sciences, Catholic University of Pusan) ;
  • Park, Gyu-Nam (Department of Clinical Laboratory Science, College of Health Sciences, Catholic University of Pusan) ;
  • Jung, Bo-Kyoung (Department of Clinical Laboratory Science, College of Health Sciences, Catholic University of Pusan) ;
  • Shin, Yu-Su (Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA) ;
  • Chang, Kyung-Soo (Department of Clinical Laboratory Science, College of Health Sciences, Catholic University of Pusan)
  • 김혜란 (부산가톨릭대학교 보건과학대학 임상병리학과) ;
  • 박규남 (부산가톨릭대학교 보건과학대학 임상병리학과) ;
  • 정보경 (부산가톨릭대학교 보건과학대학 임상병리학과) ;
  • 신유수 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 장경수 (부산가톨릭대학교 보건과학대학 임상병리학과)
  • Received : 2016.10.29
  • Accepted : 2016.12.29
  • Published : 2016.12.30
Download PDF
⟨ Previous Next ⟩

Abstract

Cymbidium is one of perennial herbs belonging to the Orchidaceae and is known as a medicinal plant. However, its scientific data are insufficient. The purpose of this study is to extract from root and stem of Cymbidium, to investigate the biological effects of them. Cymbidium antibacterial effects of the extracts were performed by antibacterial test against Staphylococcus aureus (S. aureus), Staphylococcus saphrophyticus (S. saprophyticus), Proteus vulgaris (P. vulgaris) and Klebsiella pneumonia (K. pneumonia). Antioxidant effects of the extracts were carried out by DPPH radical scavenging. Total phenolic contents were also determined. Moreover, Cell viability of extracts against MTT assay was cell viability against HepG2 cell and also measures Cholesterol adsorptivity of extracts. In this study, the extracts inhibited the growth of bacteria. Particularly Cymbidium root extracts by only ethanol extraction showed highest antimicrobial effect against S. aureus. The Cymbidium stem extracts by both ethanol extraction and sonication for 1 hour had higher antioxidant activites as well as total phenolic contents. Cell cytotoxicity showed higher than $50{\mu}g/mL$. Cholesterol adsorptivity showed lower than 20%. These results suggest that the Cymbidium might be a source of anti-bacterials and anti-oxidants.

심비디움은 난초과(orchidaceae)에 속하는 다년생 초(perennial herb)로 약용 식물로 알려져 있으나 이에 대한 과학적 자료가 부족한 실정이다. 본 연구의 목적은 심비디움의 뿌리, 줄기로부터 추출하여 생리 활성을 비교 분석하고자 한다. 심비디움 추출물의 항균 효과는 균 종별 특이성을 규명하기 위해 실험 균주는 그람 양성균 대표로 Staphylococcus aureus (S. aureus)와 Staphylococcus saphrophyticus (S. saprophyticus), 그람 음성균 대표로 Proteus vulgaris (P. vulgaris)와 Klebsiella pneumonia (K. pneumonia)를 사용하였다. 항산화 효과는 DPPH 라디칼 소거능 및 총 페놀 함량 시험을 수행하였다. 또한 간세포에 대한 세포독성 및 콜레스테롤 흡착능 시험을 수행하였다. 심비디움 추출물을 농도 별로 처리하여 균 성장 억제를 확인해본 결과, 심비디움 뿌리 에탄올 추출물에서 S. aureus에 대한 항균효과를 확인하였으며, 심비디움 줄기 에탄올 추출물 및 1시간 음파처리를 한 심비디움 줄기 에탄올 추출물에서 높은 항산화 효과를 확인하였다. 간세포에 대한 세포 독성은 $50{\mu}g/mL$이상의 농도로 확인하였으며, 콜레스테롤 흡착능은 20% 미만으로 미비한 결과를 확인하였다. 본 연구를 통해 심비디움 추출물의 항균 및 항산화 효과를 확인하였으며, 천연 항균 및 항산화 소재로 가능성이 높을 것으로 사료된다.

Keywords

References

  1. D. Bagchi, M. Bagchi, S. J. Stohs, D. K. Das, S. D. Ray, C. A. Kuszynski, et al. Free radicals and grape seed proanthocyanidin extract: importance in human health and disease prevention. Toxicology. 148, 187-197(2000). https://doi.org/10.1016/S0300-483X(00)00210-9
  2. D. Ivanova, R. Bakalova, D. Lazarova, V. Gadjeva, Z. Zhelev. The impact of reactive oxygen species on anticancer therapeutic strategies. Adv Clin Exp Med. 22, 899-908(2013).
  3. H. R. Kim, G. N. Park, B. K. Jung, W. J. Yoon, Y. H. Jung, K. S. Chang. Antioxidant activity of solvent fractions from Distylium racemosum in Jeju. Korean J Clin Lab Sci. 48, 62-67(2016). https://doi.org/10.15324/kjcls.2016.48.2.62
  4. A. Manayi, M. Khanavi, S. Saeidnia, E. Azizi, M. R. Mahmoodpour, F. Vafi, et al. Biological activity and microscopic characterization of Lythrum salicaria L. Daru. 21, 61(2013). https://doi.org/10.1186/2008-2231-21-61
  5. J. W. Lampe. Health effects of vegetables and fruit: assessing mechanisms of action in human experimental studies. Am J Clin Nutr. 70, S475-490(1999). https://doi.org/10.1093/ajcn/70.3.475s
  6. M. H. Hsieh, Z. J. Pan, P. H. Lai, H. C. Lu, H. H. Yeh, C. C. Hsu, et al. Virus-induced gene silencing unravels multiple transcription factors involved in floral growth and development in Phalaenopsis orchids. J Exp Bot. 64, 3869-3884(2013). https://doi.org/10.1093/jxb/ert218
  7. C. Simmler, C. Antheaume, A. Lobstein. Antioxidant biomarkers from Vanda coerulea stems reduce irradiated HaCaT PGE-2 production as a result of COX-2 inhibition. PLoS One. 5, e13713(2010). https://doi.org/10.1371/journal.pone.0013713
  8. S. K. Sharma, S. Kumaria, P. Tandon, R. R Satyawada. Comparative karyomorphological study of some Indian Cymbidium Swartz, 1799 (Cymbidieae, Orchidaceae). Comp Cytogenet. 6, 453-465(2012). https://doi.org/10.3897/compcytogen.v6i4.3461
  9. H. Motomura, M. A. Selosse, F. Martos, A. Kagawa, T. Yukawa. Mycoheterotrophy evolved from mixotrophic ancestors: evidence in Cymbidium (Orchidaceae). Ann Bot. 106, 573-581(2010). https://doi.org/10.1093/aob/mcq156
  10. P. Mohanty, S. Paul, M. C. Das, S. Kumaria, P. Tandon. A simple and efficient protocol for the mass propagation of Cymbidium mastersii: an ornamental orchid of Northeast India. AoB Plants. 2012, pls023(2012).
  11. S. F. Lo, S. M. Nalawade, V. Mulabagal, S. Matthew, C. L. Chen, C. L. Kuo, et al. In vitro propagation by asymbiotic seed germination and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity studies of tissue culture raised plants of three medicinally important species of dendrobium. Biol Pharm Bull. 27, 731-735(2004). https://doi.org/10.1248/bpb.27.731
  12. A. Luo, Y. Fan. In vitro antioxidant of a water-soluble polysaccharide from Dendrobium fimhriatum Hook. var. oculatum Hook. Int J Mol Sci. 12, 4068-4079(2011). https://doi.org/10.3390/ijms12064068
  13. A. Luo, Z. Ge, Y. Fan, A. Luo, Z. Chun, X. He. In vitro and in vivo antioxidant activity of a water-soluble polysaccharide from Dendrobium denneanum. Molecules. 16, 1579-1592(2011). https://doi.org/10.3390/molecules16021579
  14. F. Jiang, W. Li, Y. Huang, Y. Chen, B. Jin, N. Chen, et al. Antioxidant, antityrosinase and antitumor activity comparison: the potential utilization of fibrous root part of Bletilla striata (Thunb.) Reichb.f. PLoS One. 8, e58004(2013). https://doi.org/10.1371/journal.pone.0058004
  15. I. S. Kim, D. K. Choi, H. J. Jung. Neuroprotective effects of vanillyl alcohol in Gastrodia elata Blume through suppression of oxidative stress and anti-apoptotic activity in toxin-induced dopaminergic MN9D cells. Molecules. 16, 5349-5361(2011). https://doi.org/10.3390/molecules16075349
  16. M. A. Howlader, M. Alam, KhT. Ahmed, F. Khatun, A. S. Apu. Antinociceptive and anti-inflammatory activity of the ethanolic extract of Cymbidium aloifolium (L.). Pak J Biol Sci. 14, 909-911(2011). https://doi.org/10.3923/pjbs.2011.909.911
  17. H. J. Jo, Y. S. Shin, G. N. Park, K. S. Chang. Inhibition of hepatitis C virus (HCV) genotype 2a RNA replication by extracts of medicinal herbs with anticholesterol effects. Journal of Medicinal Plants Research. 7, 1089-1099(2013).
  18. X. Yang, C. Tang, P. Zhao, G. Shu, Z. Mei. Antimicrobial constituents from the tubers of Bletilla ochracea. Planta Med. 78, 606-610(2012). https://doi.org/10.1055/s-0031-1298264
  19. J. Liu, J. Zhang, Y. Shi, S. Grimsgaard, T. Alraek, V. Fonnebo V. Chinese red yeast rice (Monascus purpureus) for primary hyperlipidemia: a meta-analysis of randomized controlled trials. Chin Med. 1, 4(2006). https://doi.org/10.1186/1749-8546-1-4