Journal of the Korean Applied Science and Technology (한국응용과학기술학회지)

The Korean Society of Applied Science and Technology (한국응용과학기술학회)
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Biological Effects of Vinca minor extract; Tyrosinase inhibition, stimulation of ROS generation and increasement of cell migration activity in keratinocytes

  • Kim, Jun-Sub (Department of Biotechnology, Korea National University of Transportation) ;
  • Yu, Il-Hwan (Department of Biotechnology, Korea National University of Transportation) ;
  • Joo, Ji-Hye (Department of Biotechnology, Korea National University of Transportation) ;
  • Nam, Gyeong Hoe (Department of Biotechnology, Korea National University of Transportation) ;
  • Jung, Kyung-Hwan (Department of Biotechnology, Korea National University of Transportation) ;
  • Chung, Young Soo (Beauty Cosmetic Co., Ltd.) ;
  • Lee, Hyang-Yeol (Department of Biotechnology, Korea National University of Transportation)
  • Received : 2016.11.24
  • Accepted : 2016.12.28
  • Published : 2016.12.30
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Abstract

Vinca alkaloids from plant Vinca minor have been investigated for their effects of tyrosinase inhibition, stimulation of ROS generation and increasement of cell migration activity. The methanolic crude extract and the water-soluble fraction exhibited $IC_{50}$ value of 3.1 mg/mL and 2.1 mg/mL. Vinca minor extract treatment significantly increased ROS levels in HaCaT cells, in a concentration-dependent manner. Treatments of Vinca minor extract led to increase wound closure when compared with non-treatment. Low dose (0.1% or 0.3%) of extracts have not significantly affected, compared with that in controls. By contrast, 0.5% extract have dramatic effect on wound healing activity of keratinocytes. Effects of Vinca minor extract in a filter-based cell mobility assay appear similar to that of wound closure assay, which suggests that the Vinca minor extract have wound healing effects on skin.

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References

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