Korean journal of applied entomology (한국응용곤충학회지)

Korean Society of Applied Entomology (한국응용곤충학회)
권호 표지
DOI QR코드

DOI QR Code

Occurrence of the Mite Pathogenic Fungus Neozygites floridana on Two Spotted Spider Mite (Tetranychus urticae) in Korea

점박이응애에서 병원성 곰팡이 Neozygites floridana의 발생

  • Choi, Seon-U (Jeollabuk-do Agricultural Research and Extension Services) ;
  • Lee, Gong-Jun (Jeollabuk-do Agricultural Research and Extension Services) ;
  • Moon, Young-Hun (Jeollabuk-do Agricultural Research and Extension Services) ;
  • Seo, Kyoung-Won (Jeollabuk-do Agricultural Research and Extension Services) ;
  • Kang, Chan-Ho (Jeollabuk-do Agricultural Research and Extension Services) ;
  • Kim, Jin-Ho (Jeollabuk-do Agricultural Research and Extension Services) ;
  • Kim, Jae-Su (Department of Agricultural Biology, College of Agriculture & Life Scinece, Chonbuk National University)
  • Received : 2016.09.07
  • Accepted : 2016.11.07
  • Published : 2016.12.01
Download PDF
⟨ Previous Next ⟩

Abstract

An entomopathogenic fungus was isolated from the two-spotted spider mite (Tetranychus urticae) in a rearing house, and identified as Neozygites floridana (Entomophthorales: Neozygitaceae). A high infection rate induced by N. floridana could increase the price of the natural enemy. The body color of mites infected by this fungus changed to red or orange and swelling occurred. Fungal conidia were discharged into the webbing produced by the spider mites, making it relatively easy to infect the mites. Primary conidia were pear shaped and capilliconidia almond shaped. The fungus could not be cultured on solid media (PDA, SDAY, or EYSDA), but could possibly be cultured in liquid media (Grace's insect tissue culture medium + 5% fetal bovine serum). Kidney beans were supplied as food for T. urticae; the mite infection rate in a kidney bean canopy was about 36.1%. The density of infected mites was higher on the underside than on the upper side of leaves. Based on the results of this survey, we need to identify methods of fungal control for natural enemy production and biological control agents for T. urticae for effective crop management.

충남 논산시의 천적생산업체인 세실의 점박이응애 사육장에서 발견된 곰팡이를 조사한 결과 Neozygites floridana 1종이 동정되었다. 종 동정은 형태학적 현미경적 특징과 DNA 염기서열 분석을 통해 이루어졌다. 곤충조직배양용 전용배지(Grace's insect tissue culture medium + 5% fetal bovine serum)에서 이 균의 생존을 확인할 수 있었다. 강낭콩 군락 내에서의 감염률은 36.1%이었다. 점박이응애는 잎의 윗면보다 뒷면에 상대적으로 높게 밀집되어 있었다. 곰팡이의 감염률은 윗면보다 뒷면에서 매우 높게 나타나, 균 발생 진단을 위해서는 잎 윗면보다는 뒷면을 확인하는 것이 요구된다. 이 균에 대하여 천적 생산을 위한 방제방법과 더불어 작물을 가해하는 점박이응애 방제를 위한 생물적 조절인자로서의 가능성에 대한 연구가 필요하다.

Keywords

References

  1. Ahn, K.S., Lee, S.Y., Lee, K.Y., Lee, Y.S., Kim, G.H., 2004. Selective toxicity of pesticides to the predatory mite, Phytoseiulus persimilis and control effects of the two-spotted spider mite, Tetranychus urticae by predatory mite and pesticide mixture on rose. Korean J. Appl. Entomol. 43, 71-79.
  2. Altschul, S.F., Madden, T.L., Schaffer, A.A., Zhang, J., Zhang, Z., Miller, W., Lipman, D.J., 1997. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res. 25, 3389-3402. https://doi.org/10.1093/nar/25.17.3389
  3. Delalibera, I., Hajek, A.E., Humber, R.A., 2003. Use of cell culture media for cultivation of the mite pathogenic fungi Neozygites tanajoae and Neozygites floridana. J. Invertebr. Pathol. 84, 119-127. https://doi.org/10.1016/j.jip.2003.09.005
  4. Fisher, F.E., 1951. An Entomophthora attacking citrus red mite. Fla. Entomol, 83-88.
  5. Humber, R.A., 1997. Fungi: identification. Manual of techniques in insect pathology. Academic, San Diego, CA, 153-185.
  6. Keller, S., 1997. The genus Neozygites (Zygomycetes, Entomophthorales) with special reference to species found in tropical regions. SYDOWIA-HORN-49, 118-146.
  7. Kim, D.S., Lee, J.H., 1994. Foraging behavior of Amblyseius longispinosus (Acarina: Phytoseiidae) for Tetranychus urticae (Acarina: Tetranychidae) Eggs. Korean J. Appl. Entomol. 33, 33-38.
  8. Kim, S.S., Kim, D.I., Lee, S.C., 1992. Joint toxic action of acaricide mixtures to the dicofol and the ethion resistant strain of Tetranychus urticae. Korean J. Entomol. 22, 243-250.
  9. Lim, J.R., Choi, S.U., Kim, J.H., Moon, H.C., Lee, K.K., Kim, D.H., Ryu, J., Lee, S.K., Hwang, C.Y., 2008. Economic Injury Levels of Tetranychus urticae Koch (Acari, Tetranychidae) Infesting Eggplant in Greenhouse. Korean J. Appl. Entomol. 47, 395-400. https://doi.org/10.5656/KSAE.2008.47.4.395
  10. Ribeiro, A.E.L., Gondim, M.G.C., Calderan, E., Delalibera, I., 2009. Host range of Neozygites floridana isolates (Zygomycetes: Entomophthorales) to spider mites. J. Invertebr. Pathol. 102, 196-202. https://doi.org/10.1016/j.jip.2009.08.012
  11. Van der Geest, L., Elliot, S., Breeuwer, J., Beerling, E., 2000. Diseases of mites. Experimental & applied acarology 24, 497-560. https://doi.org/10.1023/A:1026518418163
  12. Wekesa, V., Moraes, G., Ortega, E., Delalibera, I., 2010. Effect of temperature on sporulation of Neozygites floridana isolates from different climates and their virulence against the tomato red spider mite, Tetranychus evansi. J. Invertebr. Pathol. 103, 36-42. https://doi.org/10.1016/j.jip.2009.10.003
  13. Wekesa, V., Vital, S., Silva, R., Ortega, E., Klingen, I., Delalibera, I., 2011. The effect of host plants on Tetranychus evansi, Tetranychus urticae (Acari: Tetranychidae) and on their fungal pathogen Neozygites floridana (Entomophthorales: Neozygitaceae). J. Invertebr. Pathol. 107, 139-145. https://doi.org/10.1016/j.jip.2011.04.003
  14. Yoon, C.S., Lee, M.H., Lee, S.H., Yoo, J.K., Lee, J.O., 1999. First record of the entomopathogenic fungus Neozygites fresenii on the aphid in Korea. Kor. J. Mycol. 27, 66-67.