Korean journal of applied entomology (한국응용곤충학회지)

Korean Society of Applied Entomology (한국응용곤충학회)
권호 표지
DOI QR코드

DOI QR Code

Molecular Identification of the Dominant Species of Dark-winged Fungus Gnat (Diptera: Sciaridae) from Button Mushroom (Agaricus bisporus) in Korea

국내 양송이버섯 재배 중 발생하는 버섯파리류 분자생물학적 종 동정

  • Yoon, Jung-Beom (Horticultural and herbal crop environment division, National Institute of Horticultural and Herbal Science, RDA) ;
  • Kim, Hyeong-Hwan (Horticultural and herbal crop environment division, National Institute of Horticultural and Herbal Science, RDA) ;
  • Jung, Chung-Ryul (Forest medical resources research center, National Institute of Forest Science) ;
  • Kang, Min-Gu (Gyeongbuk-do Agricultural Research and extension Services) ;
  • Kwon, Sun-Jung (Horticultural and herbal crop environment division, National Institute of Horticultural and Herbal Science, RDA) ;
  • Kim, Dong-Hwan (Horticultural and herbal crop environment division, National Institute of Horticultural and Herbal Science, RDA) ;
  • Yang, Chang-Yeol (Horticultural and herbal crop environment division, National Institute of Horticultural and Herbal Science, RDA) ;
  • Seo, Mi-Hye (Horticultural and herbal crop environment division, National Institute of Horticultural and Herbal Science, RDA)
  • 윤정범 (국립원예특작과학원 원예특작환경과) ;
  • 김형환 (국립원예특작과학원 원예특작환경과) ;
  • 정충렬 (국립산림과학원 산림약용자원연구소) ;
  • 강민구 (경상북도농업기술원 농업환경과) ;
  • 권선정 (국립원예특작과학원 원예특작환경과) ;
  • 김동환 (국립원예특작과학원 원예특작환경과) ;
  • 양창열 (국립원예특작과학원 원예특작환경과) ;
  • 서미혜 (국립원예특작과학원 원예특작환경과)
  • Received : 2016.08.29
  • Accepted : 2016.11.08
  • Published : 2016.12.01
Download PDF
⟨ Previous Next ⟩

Abstract

The dark-winged fungus gnats are one of the most serious fly pests attacking the mushroom cultivation in Korea. They cause severe damage to the artificial sawdust beds used to cultivate mushroom, and reduce the production of button mushroom, Agaricus bisporus, in greenhouses. In this study, we collected nine species of the mushroom flies in order to identify the dominant species of the dark-winged fungus gnat attacking the A. bisporus plantation using the yellow sticky trap in Buyeo-gun, Boryeong-gun, Yongin-si and Chilgok-gun from April to June 2015. The collected samples were used to determine the DNA sequence of the cytochrome c oxidase subunit I (COI) of the nine different species by DNA barcoding. The sequencing results showed that Lycoriella ingenua was the dominant dark-winged fungus gnat species destroying A. bisporus cultivated on the artificial sawdust beds in Korea.

버섯파리는 국내 양송이 균상 재배지에서 발생하여 버섯 균사와 자실체를 가해하고 간접적으로는 병을 매개하여 피해는 해마다 증가하고 있다. 부여군 세도면, 보령시 성주면, 용인시 남사면, 칠곡군 약목면 등 총 4개 지역의 버섯 재배사에서 끈끈이트랩을 이용하여 채집한 버섯파리 성충을 DNA barcoding 방법으로 Cytochrome Oxidase I (COI)의 염기서열 분석을 통해 종 동정을 실시하였다. 그 결과 4개 조사지역의 양송이에서 발생하는 버섯파리는 모두 긴수염버섯파리(Lycoriella ingenua)로 동정되었다.

Keywords

References

  1. Bae, J.S., Kim, I.S., Kim, S.R., Jin, B.R., Sohn, H.D., 2001. Mitochondrial DNA sequence variation of the mushroom pest flies, Lycoriella mali (Diptera: Sciaridae) and Coboldia fuscipes (Diptera: Scatopsidae), in Korea. Appl. Entomol. Zool. 36(4), 451-457. https://doi.org/10.1303/aez.2001.451
  2. Chang, H.Y., 2003. http://m.blog.daum.net/kenny7978/136 (accessed on 16 Aug, 2016).
  3. Fletcher, J.T., Gaze, R.H., 2008. Mushroom pest and disease control, A colour handbook. Manson publishing, UK, pp. 145-165.
  4. Folmer O., Black, M., Hoef, W., Lutz, R., Vrijenhoek, R., 1994. DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Molecular Marine Biology and Biotechnology. 3, 294-299.
  5. Frank, M., 1999. Revision der palaarktischen Trauermucken (Diptera, Sciaridae) unter besonderer Berucksichtigung der deutschen Fauna. Inauguraldissertation, 355.
  6. Gotoh, T., Nakamuta, K., Tokoro, M., Nakashima, T., 1999. Copulatory behavior and sex pheromones in sciarid fly, Lycoriella mali (Fitch) (Sciaridae: Diptera). Jpn. J. Appl. Entomol. Zool. 43, 181-184. https://doi.org/10.1303/jjaez.43.181
  7. Gyeonggido Agricultural Research & Extension Services Mushroom research station, 2008. The right to know mushroom. Naturehuman, Gyeonggido, 172.
  8. Jhune, C.S., Jang, K.Y., Jeong, J.C., Lee, C.J., Kong, W.S., Yoo, Y.B., 2010. Field research of cultivation technique for stable production of common mushroom (Agaricus bisporus). J Mushroom. 8(3), 122-130.
  9. Kim, G.H., Yoo, J.S., Koo, C.D., Lee, S.G., Park, J.D., 2001. Selection of insecticides for controlling Lycoriella mali in Letinula edodes sawdust cultivation. The Korean Society Of Pesticide Science. 5, 62-66.
  10. Kim, H.H., Cho, M.R., Kang, T.J., Ahn, S.J., Lee, C.J., Cheong, J.C., 2012. Damage and biological control of dark winged fungus gnats, Lycoriella ingenua (Diptera: Sciaridae) in a shiitake cultivation. J Mushrooms. 10(4), 184-190.
  11. Kim, H.H., Choo, H.Y., Lee, D.H., Lee, H.S., Jeon, H.Y., Ha, P.J., 2004. Biological control of Mycophila speyeri Barnes (Diptera: Cecidomyiidae) using Korean Steinernema and Heterorhabditis isolates in Pleurotus ostreatus cultivation house. Kor. J. Appl. Entomol. 43(3), 233-239.
  12. Kim, H.H., Kim, D.H., Jung, Y.H., Yang, C.Y., Kang, T.K., Jeon, S.W., 2014. Attract effect of mushroom flies with different wavelength of light emitting diode (LED). J Mushrooms. 12(4), 375-378. https://doi.org/10.14480/JM.2014.12.4.375
  13. Kim, H.K., Lee, B.J., Kim, Y.G., Yun, Y.U., Yang, E.S., Kim, H.G., 2010. Study of the composting method using wheat straw on Agaricus bisporus cultivation. J Mushrooms. 8(1), 33-36.
  14. Kim, K.C., Hwang, C.Y., 1996. An investigation of insect pest on the mushroom (Lentinus edode, Pleurotus ostreatus) in south region of Korea. Kor. J. Appl. Entomol. 35, 45-51.
  15. Kim, S.R., Choi, K.H., Cho, E.S., Yang, W.J., Jin, B.R., Sohn, H.D., 1999. An investigation of the major dipteran pests on the oyster mushroom (Pleurotus ostreatus) in Korea. Kor. J. Appl. Entomol. 38, 41-46.
  16. Kitajima, H., Kawashima, Y., 2013. Artificial diet for larvae of the mushroom fly Neoempheria ferruginea and determination of larval instars by head capsule widths. Jpn. J. Appl. Entomol. Zool. 57(2), 79-84. https://doi.org/10.1303/jjaez.2013.79
  17. Kwon, S.J., Kim, H.H., Song, J.S., Kim, D.W., Cho, M.R., Yang, C.Y., Kang, T.J., Ahn, S.J., Jeon, S.W., 2013. Molecular identification of fungus gnats from shiitake mushroom in Korea. J Mushrooms. 11(4), 201-207. https://doi.org/10.14480/JM.2013.11.4.201
  18. Lee, B.E., Yoon, M.H., 2014. Effects of microorganism density and mushroom yields according to the sterilization of casing soils at the cultivation of button mushrooms. Journal of Mushrooms. 12(3), 220-225. https://doi.org/10.14480/JM.2014.12.3.220
  19. Lee, B.J., Lee, M.A., Kim, Y.G., Lee, K.W., Lee, B.E., Seo, G.S., 2015. Damages and developmental characteristics of fungus gnat, Lycoriella ingenua (Diptera: Sciaridae) in button mushroom cultivation. J Mushrooms. 13(2), 145-150. https://doi.org/10.14480/JM.2015.13.2.145
  20. Lee, C.J., Yoo, Y.M., Jhune, C.S., Cheong, J.C., Moon, J.W., Kong, W.S., Suh, J.S., Kim, Y.G., Lee, H.S., 1999. The control of oyster mushrooms. Journal of Mushrooms. 3(2), 141-149.
  21. Lee, H.S., Kim, K.C., Park, C.G., Shin, W.K., 1999. Description of fungus gnat, Lycoriella mali Fitch (Diptera: Sciaridae) from Korea. Kor. J. Appl. Entomol. 38, 209-212.
  22. Lee, H.S., Kim, K.J., 2003. Report on Mycophila speyeri Barnes (Diptera: Cecidomyiidae) as a Pest of Mushroom Cultivation in Korea. Kor. J. Appl. Entomol. 42(1), 71-75.
  23. MAFRA, Ministry of Agriculture, Food and Rural Affairs. 2000-2013. Major statistics of Agriculture, Food and Rural Affairs.
  24. Mariusz, L., Agnieszka, S., Andrzej, B., 2004. Biology and morphometry of Lycoriella ingenua (Diptera: Sciaridae). BIOL. LETT. 2004, 41(1), 41-50.
  25. Menzel, F., Smith, J.E., Colauto, N.B., 2003. Bradysia difformis Frey and Bradysia ocellaris (Comstock): two additional neotropical species of black fungus gnats (Diptera: Sciaridae) of economic importance: a redescription and review. Ann. Entomol. 96, 448-457. https://doi.org/10.1603/0013-8746(2003)096[0448:BDFABO]2.0.CO;2
  26. Shamshad, A., 2012. The development of intergrated pest management for the control of mushroom sciarid flies, Lycoriella ingenua (Dufor) and Bradysia ocellaris (Comstock), in cultivated mushrooms. Pest Manag Sci. 66, 1063-1074.
  27. Shin, S.G., Lee, H.S., Lee, S.H., 2012. Dark winged fungus gnats (Diptera: Sciaridae) collected from shiitake mushroom in Korea. J Asia-Pacific Entomol. 15, 174-181. https://doi.org/10.1016/j.aspen.2011.09.005
  28. Tamura, K., Dudley, J., Nei, M., Kumar, S., 2007. Mega4: Molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol. Biol. Evol. 24, 1596-1599. https://doi.org/10.1093/molbev/msm092
  29. Tamura, K., Peterson, D., Peterson, N., Stecher, G., Nei, M., Kumar, S., 2011. Mega5: Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol. Biol Evol. 28, 2731-2739. https://doi.org/10.1093/molbev/msr121
  30. White, P.F., Smith, J.E., 2000. Population development of mushroom pests on species and strains of Agaricus. Science and Cultivation of Edible Fungi, van Griensven(ed.), 707-712.
  31. Yi, J.H., Park, I.K., Choi, K.S., Shin, S.C., Ahn, Y.J., 2008. Toxicity of medicinal plant extracts to Lycoriella ingenua (Diptera: Sciaridae) and Coboldia fuscipes (Diptera: Scatopsidae). J. Asia Pac. Entomol. 11, 221-223. https://doi.org/10.1016/j.aspen.2008.09.002
  32. You, C.H., 2003. History of mushroom industry in Korea. J Mushrooms. 1(1), 1-8.

Cited by

  1. Control of Dark-winged Fungus Gnats (Lycoriella ingenua) using Mist Fogger with Natural Pyrethrum (Tanacetum cinerariifolium) vol.21, pp.3, 2017, https://doi.org/10.7585/kjps.2017.21.3.279