Nutrition Research and Practice

The Korean Nutrition Society (한국영양학회)
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Characteristics of nonalcoholic fatty liver disease induced in wistar rats following four different diets

  • Fakhoury-Sayegh, Nicole (Department of Nutrition, Faculty of Pharmacy, Saint Joseph University) ;
  • Trak-Smayra, Viviane (Department of Pathology, Faculty of Medicine, Saint Joseph University) ;
  • Khazzaka, Aline (Department of Surgical Research, Faculty of Medicine, Saint Joseph University) ;
  • Esseily, Fady (Department of Laboratory Sciences, Faculty of Public Health II, Lebanese University) ;
  • Obeid, Omar (Department of Nutrition and Food Sciences, Faculty of Agricultural and Food Sciences, American University of Beirut) ;
  • Lahoud-Zouein, May (Department of Laboratory Sciences, Faculty of Public Health II, Lebanese University) ;
  • Younes, Hassan (Department of Nutrition and Health Sciences, Institut Polytechnique LaSalle Beauvais)
  • Received : 2014.05.20
  • Accepted : 2015.02.11
  • Published : 2015.08.01
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Abstract

BACKGROUND/OBJECTIVES: The prevalence of nonalcoholic fatty liver disease (NAFLD) has increased worldwide in parallel with overnutrition characterized by high-fat and high-carbohydrate intake. Our objective was to establish, in 16 weeks, a model of NAFLD in Wistar pathogen-free rats following four dietary types. MATERIALS/METHODS: Forty (6 weeks old) healthy Wistar male rats, weighing an average of 150 g were randomly divided into four groups of ten and assigned a diet with the same quantity (15 g/rat/day), but with different composition. The moderate-fat (MF) group was fed a moderate-fat diet (31.5% fat and 50% carbohydrates), the high-fat (HF) group was fed a fat-rich diet (51% fat), the high-sucrose (HS) group and the high-fructose (HFr) group were fed a carbohydrate-rich diet (61%). The carbohydrate contents of the HS group was composed of 60.3% sucrose while that of the HFr group was composed of 59.3% fructose. RESULTS: At week 16, the HF group had the highest percentage of cells enriched in fat (40%) and the highest weight and liver weight (P < 0.05). The HFr group showed significantly higher levels of serum triglycerides, alanine aminotransferase and adiponectin at week 16 as compared to week 1 (P < 0.05). CONCLUSIONS: The 15 g/rat/day diet composed of 51% fat or 61% carbohydrates enriched mainly in fructose may induce characteristics of NAFLD in rats.

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References

  1. Paschos P, Paletas K. Non alcoholic fatty liver disease and metabolic syndrome. Hippokratia 2009;13:9-19.
  2. Amacher DE. Strategies for the early detection of drug-induced hepatic steatosis in preclinical drug safety evaluation studies. Toxicology 2011;279:10-8. https://doi.org/10.1016/j.tox.2010.10.006
  3. Lieber CS, Leo MA, Mak KM, Xu Y, Cao Q, Ren C, Ponomarenko A, DeCarli LM. Model of nonalcoholic steatohepatitis. Am J Clin Nutr 2004;79:502-9. https://doi.org/10.1093/ajcn/79.3.502
  4. Marchesini G, Brizi M, Bianchi G, Tomassetti S, Bugianesi E, Lenzi M, McCullough AJ, Natale S, Forlani G, Melchionda N. Nonalcoholic fatty liver disease: a feature of the metabolic syndrome. Diabetes 2001;50:1844-50. https://doi.org/10.2337/diabetes.50.8.1844
  5. Farrell GC, Larter CZ. Nonalcoholic fatty liver disease: from steatosis to cirrhosis. Hepatology 2006;43:S99-112. https://doi.org/10.1002/hep.20973
  6. Basaranoglu M, Kayacetin S, Yilmaz N, Kayacetin E, Tarcin O, Sonsuz A. Understanding mechanisms of the pathogenesis of nonalcoholic fatty liver disease. World J Gastroenterol 2010;16:2223-6. https://doi.org/10.3748/wjg.v16.i18.2223
  7. Day CP, James OF. Steatohepatitis: a tale of two "hits"? Gastroenterology 1998;114:842-5. https://doi.org/10.1016/S0016-5085(98)70599-2
  8. Dowman JK, Tomlinson JW, Newsome PN. Pathogenesis of nonalcoholic fatty liver disease. QJM 2010;103:71-83. https://doi.org/10.1093/qjmed/hcp158
  9. Postic C, Girard J. Contribution of de novo fatty acid synthesis to hepatic steatosis and insulin resistance: lessons from genetically engineered mice. J Clin Invest 2008;118:829-38. https://doi.org/10.1172/JCI34275
  10. Kawasaki T, Igarashi K, Koeda T, Sugimoto K, Nakagawa K, Hayashi S, Yamaji R, Inui H, Fukusato T, Yamanouchi T. Rats fed fructoseenriched diets have characteristics of nonalcoholic hepatic steatosis. J Nutr 2009;139:2067-71. https://doi.org/10.3945/jn.109.105858
  11. Xu ZJ, Fan JG, Ding XD, Qiao L, Wang GL. Characterization of high-fat, diet-induced, non-alcoholic steatohepatitis with fibrosis in rats. Dig Dis Sci 2010;55:931-40. https://doi.org/10.1007/s10620-009-0815-3
  12. Abdelmalek MF, Suzuki A, Guy C, Unalp-Arida A, Colvin R, Johnson RJ, Diehl AM; Nonalcoholic Steatohepatitis Clinical Research Network. Increased fructose consumption is associated with fibrosis severity in patients with nonalcoholic fatty liver disease. Hepatology 2010; 51:1961-71. https://doi.org/10.1002/hep.23535
  13. Animal Research Review Panel (AU). Guideline 20: Guidelines for the Housing of Rats in Scientific Institutions. Sydney: Animal Research Review Panel; 2004.
  14. Reeves PG, Nielsen FH, Fahey GC Jr. AIN-93 purified diets for laboratory rodents: final report of the American Institute of Nutrition Ad Hoc Writing Committee on the reformulation of the AIN-76A rodent diet. J Nutr 1993;123:1939-51. https://doi.org/10.1093/jn/123.11.1939
  15. Kleiner DE, Brunt EM, Van Natta M, Behling C, Contos MJ, Cummings OW, Ferrell LD, Liu YC, Torbenson MS, Unalp-Arida A, Yeh M, McCullough AJ, Sanyal AJ; Nonalcoholic Steatohepatitis Clinical Research Network. Design and validation of a histological scoring system for nonalcoholic fatty liver disease. Hepatology 2005;41:1313-21. https://doi.org/10.1002/hep.20701
  16. AOAC International (US). Official Methods of Analysis of AOAC International. 18th ed. Gaithersburg (MD): AOAC International; 2005.
  17. Smedes F. Determination of total lipid using non-chlorinated solvents. Analyst 1999;124:1711-8. https://doi.org/10.1039/a905904k
  18. Subcommittee on Laboratory Animal Nutrition; Committee on Animal Nutrition; Board on Agriculture; Institute for Laboratory Animal Research; National Research Council (US). Nutrient Requirements of Laboratory Animals. 4th rev [Internet]. Washington, D.C.: National Academies Press; 1995 [cited 2014 Aug 11]. Available from: http://www.nap.edu/.
  19. Akiyama T, Tachibana I, Shirohara H, Watanabe N, Otsuki M. High-fat hypercaloric diet induces obesity, glucose intolerance and hyperlipidemia in normal adult male Wistar rat. Diabetes Res Clin Pract 1996;31:27-35. https://doi.org/10.1016/0168-8227(96)01205-3
  20. Gauthier MS, Favier R, Lavoie JM. Time course of the development of non-alcoholic hepatic steatosis in response to high-fat dietinduced obesity in rats. Br J Nutr 2006;95:273-81. https://doi.org/10.1079/BJN20051635
  21. Lustig RH. Fructose: metabolic, hedonic, and societal parallels with ethanol. J Am Diet Assoc 2010;110:1307-21. https://doi.org/10.1016/j.jada.2010.06.008
  22. Ackerman Z, Oron-Herman M, Grozovski M, Rosenthal T, Pappo O, Link G, Sela BA. Fructose-induced fatty liver disease: hepatic effects of blood pressure and plasma triglyceride reduction. Hypertension 2005;45:1012-8. https://doi.org/10.1161/01.HYP.0000164570.20420.67
  23. Ku era O, Garnol T, Lotkova H, Stankova P, Mazurova Y, Hroch M, Bolehovska R, Rousar T, Cervinkova Z. The effect of rat strain, diet composition and feeding period on the development of a nutritional model of non-alcoholic fatty liver disease in rats. Physiol Res 2011;60:317-28.
  24. Ahmed U, Redgrave TG, Oates PS. Effect of dietary fat to produce non-alcoholic fatty liver in the rat. J Gastroenterol Hepatol 2009;24: 1463-71. https://doi.org/10.1111/j.1440-1746.2009.05870.x
  25. Lanthier N, Horsmans Y, Leclercq IA. The metabolic syndrome: how it may influence hepatic stellate cell activation and hepatic fibrosis. Curr Opin Clin Nutr Metab Care 2009;12:404-11. https://doi.org/10.1097/MCO.0b013e32832c7819
  26. Saha JK, Xia J, Grondin JM, Engle SK, Jakubowski JA. Acute hyperglycemia induced by ketamine/xylazine anesthesia in rats: mechanisms and implications for preclinical models. Exp Biol Med (Maywood) 2005;230:777-84. https://doi.org/10.1177/153537020523001012
  27. Bugianesi E, Gastaldelli A, Vanni E, Gambino R, Cassader M, Baldi S, Ponti V, Pagano G, Ferrannini E, Rizzetto M. Insulin resistance in non-diabetic patients with non-alcoholic fatty liver disease: sites and mechanisms. Diabetologia 2005;48:634-42. https://doi.org/10.1007/s00125-005-1682-x
  28. Utzschneider KM, Kahn SE. Review: the role of insulin resistance in nonalcoholic fatty liver disease. J Clin Endocrinol Metab 2006;91:4753-61. https://doi.org/10.1210/jc.2006-0587
  29. Yamauchi T, Kamon J, Minokoshi Y, Ito Y, Waki H, Uchida S, Yamashita S, Noda M, Kita S, Ueki K, Eto K, Akanuma Y, Froguel P, Foufelle F, Ferre P, Carling D, Kimura S, Nagai R, Kahn BB, Kadowaki T. Adiponectin stimulates glucose utilization and fatty-acid oxidation by activating AMP-activated protein kinase. Nat Med 2002;8:1288-95. https://doi.org/10.1038/nm788
  30. Pagano C, Soardo G, Esposito W, Fallo F, Basan L, Donnini D, Federspil G, Sechi LA, Vettor R. Plasma adiponectin is decreased in nonalcoholic fatty liver disease. Eur J Endocrinol 2005;152:113-8. https://doi.org/10.1530/eje.1.01821
  31. Kamari Y, Grossman E, Oron-Herman M, Peleg E, Shabtay Z, Shamiss A, Sharabi Y. Metabolic stress with a high carbohydrate diet increases adiponectin levels. Horm Metab Res 2007;39:384-8. https://doi.org/10.1055/s-2007-976534
  32. Bray GA. How bad is fructose? Am J Clin Nutr 2007;86:895-6. https://doi.org/10.1093/ajcn/86.4.895
  33. Tetri LH, Basaranoglu M, Brunt EM, Yerian LM, Neuschwander-Tetri BA. Severe NAFLD with hepatic necroinflammatory changes in mice fed trans fats and a high-fructose corn syrup equivalent. Am J Physiol Gastrointest Liver Physiol 2008;295:G987-95. https://doi.org/10.1152/ajpgi.90272.2008
  34. Fried SK, Rao SP. Sugars, hypertriglyceridemia, and cardiovascular disease. Am J Clin Nutr 2003;78:873S-880S. https://doi.org/10.1093/ajcn/78.4.873S
  35. Getz GS, Bartley W, Stirpe F, Notton BM, Renshaw A. The lipid composition of rat liver. Biochem J 1961;80:176-81. https://doi.org/10.1042/bj0800176

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