Journal of the Korean Applied Science and Technology (한국응용과학기술학회지)

The Korean Society of Applied Science and Technology (한국응용과학기술학회)
권호 표지
DOI QR코드

DOI QR Code

Physiological Activities of Cudrania tricuspidata Extracts on the Skin

꾸지뽕나무 추출물의 피부 생리 활성

  • 최학주 (대전대학교 난치성면역질환 동서생명의학연구센터(TBRC)) ;
  • 김청택 ((주)알엔에스) ;
  • 도민연 (고성군 농업기술센터) ;
  • 랑문정 (배재대학교 제약공학과)
  • Received : 2015.05.31
  • Accepted : 2015.06.13
  • Published : 2015.06.30
Download PDF
⟨ Previous Next ⟩

Abstract

This paper has shown the experimental results about the physiological activities of water-, ethanol-, ethyl acetate-soluble fractions from ethanolic extracts of leaves, stems and roots of Cudrania tricuspidata on the skin, which has been used for a long time as a traditional herb medicine in Korea and China. The effects of these fractions on the secretion of nitric oxide and cytokines from macrophage(RAW 264.7 cell) exhibited that the ethyl acetate and water fractions from leaves inhibited the release of nitric oxide, all fractions inhibited thoses of inflammatory cytokine $IL-1{\alpha}$, and the ethyl acetate fractions of leaves, stems and roots inhibited thoses of inflammatory cytokine IL-6. The only ethylacetate fraction of leaves demonstrated significantly the reduction of melanin synthesis in melanoma cells. In order to evaluate the efficacy of collagen synthesis, the treatment with extracts on the human normal fibroblast cell(CCD-986sk cell) resulted in finding that the water fractions of leaves, stems and roots and the ethanol fractions of leaves and stems showed the increased synthesis of collagen.

본 논문은 한국과 중국에서는 전통 한방약재로 오랫동안 사용된 꾸지뽕나무(Cudrania tricuspidata)의 잎, 줄기, 뿌리부분의 에탄올추출물의 물, 에탄올, 에칠아세테이트 용해성 분획물에 대한 피부 생리활성에 관한 실험결과이다. 대식세포인 macrophage(RAW 264.7 cell)에 대하여 이들 분획물들의 nitric oxide 및 염증성 사이토카인 분비 억제효과를 검토한 결과, nitric oxide 생성 억제에는 잎부위의 에칠아세테이트 분획물과 에탄올분획물이, 염증성 사이토카인인 $IL-1{\beta}$ 생성 억제에는 모든 분획물들이 효과가 있고, 염증성 사이토카인인 IL-6 생성 억제에는 잎, 줄기, 뿌리부위의 에칠아세테이트 분획물들이 효과가 있는 것으로 나타났다. 흑색종세포인 melanoma cell(B16-F10 cell)에서 멜라닌 생성억제효과를 측정한 결과, 잎의 에틸아세테이트 분획물 처리에서만 유의성 있는 억제효과를 나타내었다. 주름 개선 효과를 확인하기 위해 인체 정상 피부 섬유아세포( CCD986sk cell)에 대하여 콜라겐의 합성 촉진 효과를 측정한 결과. 잎, 줄기, 뿌리부위의 물 분획물과 잎, 줄기의 에탄올 분획물에서 콜라겐 합성 촉진 효과를 보였다.

Keywords

References

  1. Y. N. Lee, New Flora of Korea, I, p.246-247, Kyo-Hak Publishing Co. Ltd. (2006).
  2. J. S. Kim and T. Y Kim, Woody Plants of Korea, p.142, Dolbegae(2011).
  3. Doopedia(Doosan Encyclopedia), Cudrania tricuspidata, Doosan Corporation, Available From http://www.doopedia.co.kr/doopedia/master/master.do?_method=view&MAS_ IDX=101013000831679 (accessed March 24, 2015).
  4. H. J. Choi, C. T. Kim, M. Y. Do and M. J. Rang, Physiological Activities of Cudrania tricuspidata Extracts (Part 1), J, Korea Academia-Industrial Cooperation Soc., 14(8), 3911(2013).
  5. Heo-Jun, Translation Dongui Bogam, p.1972, Bub-In Culture Co.(1999).
  6. C. M. Kim, Translation Encyclopedia of Oriental, p.4166, p.3357, p.3582, Herbal Medicine, Jeong-Dam (2004).
  7. J. C. Park, H. S. Young and J. S. Choi, Constituents of Cudrania tricuspidata in Korea, YAKHAK HOEJI, 36(1), 40 (1992).
  8. S. H. Kim, N. J. Kim, J. S. Choi and J. C. Park, Determination of Flavonoid by HPLC and Biological Activities from the Leaves of Cudrania tricuspidata Bureau, J. Korean Soc, Food Sci. Nutr., 22(1), 68 (1993).
  9. Z. P. Zheng, H. Y. Tan, J. Chen and M. F. Wang, Characterization of Tyrosinase Inhibitors in the Twigs of Cudrania tricuspidata and Their Structure-Activity Relationship Study, Fitoterapia, 84, 242 (2013). https://doi.org/10.1016/j.fitote.2012.12.006
  10. I. K. Lee, K. S. Song, C. J. Kim, H. M. Kim, G. T. Oh and I. D. Yoo, Tumor Cell Growth Inhibition and Antioxydative Activity of Flavonoids from the Stem Bark of Cudrania tricuspidata, J, Korean Soc. Appl. Biol. Chem., 37(2), 105 (1994).
  11. I. K. Lee, C. J. Kim, K. S. Song, H, M. Kim, H. Koshino, M. Uramoto, and I. D. Yoo, Cytotoxic Benzyl Dihydroflavonols from Cudrania tricuspidata, Phytochemistry, 41(1), 213 (1996). https://doi.org/10.1016/0031-9422(95)00609-5
  12. Y. S. Zou, A. J. Hou, G. F. Zhu, Y. F. Chen, H. D. Sun and Q. S. Zhao, Cytotoxic isoprenylated xanthones from Cudrania tricuspidata, Bioorganic & Medicinal Chemistry, 12, 1947 (2004). https://doi.org/10.1016/j.bmc.2004.01.030
  13. B. W. Lee, J. H. Lee, S. T. Lee, H. S. Lee, W. S. Lee, T. S. Jeong and K. H. Park, Antioxidant and Cytotoxic Acvities of Xnthones from Cudrania tricuspidata, Bioorganic & Medicinal Chemistry, 15, 5548 (2005). https://doi.org/10.1016/j.bmcl.2005.08.099
  14. Y, K. Yoon, S. E. Lee, D. J. Lee, M. C. Rho, J. S. Sung, C. B. Park and Y. J. Jang, Anti-cancer Activity of Korean Local Plant Extracts Inducing Apoptosis in Various Carcinoma Cells, Natural Product Sciences, 40(1), 6 (2009).
  15. S. R. Choi, D. H. You, I. Jang, M. S. Ahn, E. J. Song, S. Y. Seo, M. K. Choi, Y. S. Kim, M. K. Kim and D. G. Choi, Cytotoxicity of Methanol Extracts from Cudrania tricuspidata Bureau, Korean J. Medicinal Crop Sci., 20(3), 153 (2012). https://doi.org/10.7783/KJMCS.2012.20.3.153
  16. B. W. Lee, N. S. Kang and K. H. Park , Isolation of Antibacterial Prenylated Flavonoids from Cudrania tricuspidata, J, Korean Soc. Appl. Biol. Chem., 47(2), 270 (2004).
  17. Y. C. Kim, J. Hur, D. H. Sohn and H. S. Kim, Antibacterial Compounds of the Root Barks of Cudrania tricuspidata, Journal of Pharmacognosy, 39(3), 246 (2008).
  18. J. Y. Cha, H. J. Kim, C. H. Chung and Y. S. Cho, Antioxidative Activities and Contents of Polyphenolic Compound of Cudrania tricuspidata, ]. Korean Soc. Food Sci. Nutr., 28(6), 1310 (1999).
  19. J. Y. Cha and Y. S. Cho, Antioxidative Activity of Extracts from Fruit of Curdrania tricuspidata, J, Korean Soc, Food Sci, Nutr,, 30(3), 547 (2001).
  20. S. R. Choi, D. H. You, J. Y. Kim, C. B. Park, D. H. Kim, and J. Ryu, Antioxidant Activity of Methanol Extracts from Cudrania tricuspidata Bureau According to Harvesting Parts and Time, Korean J Medicinal Crop Sci., 17, 115 (2009).
  21. C. H. Jeong , G. N. Choi, J. H. Kim, J. H. Kwak, H. J. Heo, K. H. Shim, B. R. Cho, Y. I. Bae and J. S. Choi, Jine-Shang. In vitro Antioxidative Activities and Phenolic Composition of Hot Water Extract from Different Parts of Cudrania tricuspidata, J, Food Sci, Nutr., 14(4), 283 (2009). https://doi.org/10.3746/jfn.2009.14.4.283
  22. K. S. Youn and J. W. Kim, Antioxidant and Angiotensin Converting Enzyme I Inhibitory Activities of Extracts from Mulberry (Cudrania tricuspidata) Fruit subjected to Different Drying Methods, J, Korean Soc, Food Sci. Nutr., 41(10), 1388 (2012). https://doi.org/10.3746/jkfn.2012.41.10.1388
  23. J. Y. Cha, H. J. Kim and Y. S. Cho, Effects of Water-Soluble Extract from Leaves of Morus alba and Cudrania tricuspidata on the Lipid Peroxidation in Tissues, J, Korean Soc, Food Sci. Nutr., 25(3), 631 (2000).
  24. J. H. Park, K. W. Lee, K. S. Sung, S. S. Kim, K. D. Cho, B. H. Lee and C. K. Han, Effect of Diets with Job's Tears and Cudrania tricuspidata Leaf Mixed-powder Supplements on Body Fat and Serum Lipid, J, Korean Soc, Food Sci. Nutr,, 41(9), 943 (2012). https://doi.org/10.3746/jkfn.2012.41.7.943
  25. W. Y. Park, J. S. Ro and K. S. Lee, Hypoglycemic Effect of Cudrania tricuspidata Root Bark, Kor. J. Pharmacogn. 32(3), 248 (2001).
  26. O. K. Kim, J. N. Ho, D. E. Nam, W. J. Jun, K. T. Hwang, J. E. Kang, O. S. Chae, and J. M. Lee, Hepatoprotective Effect of Curdrania tricuspidata Extracts against Oxidative Damage, J. Korean Soc. Food Sci. Nutr., 41(1),7 (2012). https://doi.org/10.3746/jkfn.2012.41.1.007
  27. D. H. Kwon, M. B. Kim, D. Y. Yoon, Y. H. Lee, J. W. Kim, H. G. Lee, I. S. Cha, J. S. Lim. and Y. K. Choe, Screening of Plant Resources of Anti-viral Activity, Korean J, Medicinal Crop Sci., 11(1), 24 (2003).
  28. K. W. Lee, K. S. Sung, S. S. Kim, O. H. Lee, B. H. Lee and C. K. Han, Effects of Cucurbita moschata, Adlay Seed and Cudrania tricuspidata Leaf Mixed-powder Diet Supplements on the Visceral Fat, Fecal Amount and Serum Lipid Levels of the Rats on a High-Fat Diet, Korean J. Food & Nutr., 25(4), 990 (2012). https://doi.org/10.9799/ksfan.2012.25.4.990
  29. K. B. Lee, K. S. Song, E. H. Moon, J. L. Lee and Y. C. Yoo, Adjuvant Activity of Cudrania tricuspidata Water Extracts to Enhance Antigen Specific Humoral and Cellular Immune, J, Korean Soc. Appl. Biol. Chem., 52(2), 234 (2009). https://doi.org/10.3839/jksabc.2009.042
  30. J. H. Choi, H. S. Han and Y. J. Lee, Dong-Young, The Inhibitory Effect of Cudraniae Lignum on the Collageninduced Arthritis in Mice, Kor. J. Herbology, 24(3), 121 (2009).
  31. D. E. Nam, O. K, Kim and J. M. Lee, Jeongmin, Therapeutic Effects of Curdrania tricuspidata Leaf Extract on Osteoarthritis, Korean J. Food & Nutr., 42(5), 697 (2013). https://doi.org/10.3746/jkfn.2013.42.5.697
  32. J. C. Park and H. Y. Lee, Screening of Medicinal Plants on the Anti-inflammatory Effect and Active Component, Korean J. Food & Nutr., 25(3), 523 (1996).
  33. Y. M. Kim, Cudrania tricuspidata Suppresses Mast Cell-Mediated Allergic Response In Vitro and In Vivo, YAKHAK HOEJI, 56(1), 26 (2012).
  34. S. S. Oh, E. J. Seo, H. Y. Kim, Y. B. Ryu, J. W. Lee, S. W. Gal and K. H. Park, Tyrosinase Inhibitory Xanthones from Cudrania tricuspidata, Journal of Life Science, 17(4), 476 (2007). https://doi.org/10.5352/JLS.2007.17.4.476
  35. H. J. Lee, J. R. Do, J. H. Kwon and H. K. Kim, Physiological Activities of Extracts from Different Parts of Cudrania tricuspidata, J. Korean Soc, Food Sci, Nutr., 40(7), 942 (2011). https://doi.org/10.3746/jkfn.2011.40.7.942
  36. Z. Wang, P. Zheng, H. Y. Tan, J. Chen and M. F. Wang, Characterization of Tyrosinase Inhibitors in the Twigs of Cudrania tricuspidata and Their Structure-Activity Relationship study, Fitoterapia, 84, 242 (2013). https://doi.org/10.1016/j.fitote.2012.12.006
  37. O. K. Kim, J. N. Ho, D. E. Nam, W. J. Jun and J. M. Lee, Anti-wrinkle Activity of a Curdrania tricuspidata Extract on Ultraviolet-induced Photoaging, J. Korean Soc, Food Sci, Nutr., 42(4), 608 (2013). https://doi.org/10.3746/jkfn.2013.42.4.608
  38. M. J. Rang, Anti-inflammatory and Anti-allergic Effects of Herbal Extracts on Atopic Dermatitis (Part 2), J. of the Korean Oil Chemists' Soc., 30(1), 75 (2013).
  39. D. Mosser and J. P. Edwards, Exploring the Full Spectrum of Macrophage Activation, Nature Reviews Immunology, 8, 958 (2008). https://doi.org/10.1038/nri2448
  40. M. J. Rang, Anti-inflammatory and Anti-allergic Effects of Herbal Extracts on Atopic Dermatitis (Part I), J. of the Korean Oil Chemists' Soc., 28(1), 75 (2011).
  41. J. Y. Min and Y. K. Park, Effect of Dipsaci Radix Water Extract on LPS-induced Inflammatory Response in RAW264.7 Mouse Macrophages, Kor. J. Herbology, 24(4), 189 (2009).
  42. Y. S. Kang, K. Y. Kyeong, M. J. Rang, D. H. Bae, Y. G. Lee, W. G. Cho, S. G. Choi and S. G. Han, Science of Cosmetics and Household Poroducts (I), p. 331, Shin Kwang Publishing Co. Ltd.(2008).
  43. S. H. Kim, N. J. Kim, J. S. Choi and J. C. Park, Determination of Flavonoid by HPLC and Biological Activities from the Leaves of Cudrania tricuspidata Bureau, J. Korean Soc, Food Sci. Nutr., 22(1), 68 (1993).
  44. J. C. Park, J. S. Choi and J. W. Choi, Effects of the Fractions from the Leaves, Fruits, Stems and Roots of Cudrania tricuspidata and Flavonoids on Lipid Peroxidation, Kor. J. Pharmacogn. 26(4), 377 (1995).
  45. S. Ito, A Chemist's View of Melanogenesis, Pigment Cell Res., 16, 230 (2003). https://doi.org/10.1034/j.1600-0749.2003.00037.x
  46. S. A. Park, H. M. Lee, J. H. Ha, S. H., Jeon and S. N. Park, Inhibitory Effects of Dendropanax Marbifera Leaf Extracts on Malanogenesis through Down-regulation of Tyrosinase and TRP-2, Appl. Chem. Eng., 25(5), 468 (2014). https://doi.org/10.14478/ace.2014.1058
  47. E. S. Yang, R. H. Hong and S. M. Kang, The Effect of Genistein on the Proliferation and Type I pN Collagen Synthesis in Aged Normal Human Fibroblasts, Kor. J. Microbiol. Biotechnol., 35(4), 316 (2007).
  48. O. K. Kim, J. N. Ho, D. E. Nam, W. J. Jun and J. M. Lee, Anti-wrinkle Activity of a Curdrania tricuspidata Extract on Ultraviolet-induced Photoaging, J. Korean Soc, Food Sci, Nutr., 42(4), 608 (2013). https://doi.org/10.3746/jkfn.2013.42.4.608

Cited by

  1. 노루궁뎅이버섯 균사체를 이용한 2 차 발효달팽이 추출물의 항노화 활성에 관한 연구 vol.33, pp.1, 2015, https://doi.org/10.12925/jkocs.2016.33.1.143
  2. 달팽이 발효 추출물을 이용한 마스크 팩의 피부개선효과 vol.33, pp.1, 2015, https://doi.org/10.12925/jkocs.2016.33.1.30
  3. Anti-Inflammatory Effects of Compounds from Cudrania tricuspidata in HaCaT Human Keratinocytes vol.22, pp.14, 2015, https://doi.org/10.3390/ijms22147472