Journal of Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Evaluation of 3-week Repeated Dose Oral Toxicity on Amomum tsao-ko Extract in Balb/c Mice

Balb/c 마우스에서 초과 추출물의 3주간 반복 경구투여 독성평가

  • Park, Ju-Hyeong (College of Pharmacy, Dankook University) ;
  • Cho, Young-Rak (Natural Products Research Institute, Gyeonggi Institute of Science & Technology Promotion) ;
  • Ko, Hye-Jin (Natural Products Research Institute, Gyeonggi Institute of Science & Technology Promotion) ;
  • Jeong, Wonsik (College of Pharmacy, Dankook University, Natural Products Research Institute, Gyeonggi Institute of Science & Technology Promotion) ;
  • Ahn, Eun-Kyung (Natural Products Research Institute, Gyeonggi Institute of Science & Technology Promotion) ;
  • Oh, Junho (Department of Biology, Duke University) ;
  • Oh, Joa Sub (College of Pharmacy, Dankook University, Natural Products Research Institute, Gyeonggi Institute of Science & Technology Promotion)
  • Received : 2014.12.23
  • Accepted : 2015.03.10
  • Published : 2015.06.30
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Abstract

In the present study, we investigated the oral toxicity of Amomum tsao-ko Crevost et Lemaire, (Zingiberaceae) extract in Balb/c mice (BALB, n=60) for 3 weeks. Balb/c mice (10 mice/group, 6 group, $20{\pm}2g$, 6 weeks) were orally administered for 21 days, with dosage of 250, 500, 1000, 2000 mg/kg/day. Ethanol extract of A. tsao-ko did not affect any significant change of mortality, clinical signs, organs and body weights. Also, there were not significantly difference from the naive group (control) in hematological and serum biochemical examination. Consequently, these findings indicate that 3-week treatment with the ethanol extract of A. tsao-ko was not any toxic effects in Balb/c mice and the no-observed adverse effect level (NOAEL) for oral toxicity was determined to be 2000 mg/kg/day under our experimental conditions.

본 연구는 다양한 효능을 지닌 초과(Amomum tsao-ko Crevost et Lemaire)의 안전한 이용을 위한 독성평가로 식품의약품안전처 고시 제2014-6호 '의약품등의 독성시험기준'에 맞는 독성시험법에 따라 Balb/c mouse를 이용하여 3주간 반복경구투여를 통해 초과의 안전성을 확인하고자 하였다. 3주간 반복 경구투여 후 체중, 장기중량 측정, 혈액분석 및 혈액생화학 검사를 실시하여 안전성을 확인 한 결과, 초과에 의한 특별한 증상이나 체중, 장기중량의 변화는 관찰되지 않았으며, 복대동맥으로부터 채혈한 혈액을 통한 혈구분석결과에서도 대조군과 초과 추출물 투여군 간의 통계적인 유의성을 관찰 할 수 없었다. 또한 혈청을 이용하여 간기능(GOT, GPT, LDH, ALB, TP-S, T-BIL, D-BIL), 신장기능(BUN, CRE), 지질영양 관련(TG), 전해질 관련(I.P) 지표들의 생화학분석을 수행한 결과, 대조군과 유사하게 모두 정상 범위 내의 결과를 나타내었다. 이러한 결과를 통하여 초과 추출물의 최대무독성용량은 최고 투여량인 2000 mg/kg 이상으로 판단되며, 본 연구결과는 초과의 기능성 식품, 화장품, 의약품 등 다양한 소재로서의 활용에 안전성 관련 기초자료로 이용될 수 있을 것으로 사료된다.

Keywords

References

  1. Chung TH, Shim KS, Kim DS, Lee JH, and Ma JY (2011) Single dose oral toxicity and genotoxicological safety study of Ssanghwa-tang fermented with Lactobacillus acidophyllus. J Korean Oriental Med 32, 67-83.
  2. Hwang IY, Hwang SA, and Jeong CS (2013) Effects of Kalopanax pictus extracts and their related origin on gastric lesions. J Fd Hyg Safety 28, 367-75. https://doi.org/10.13103/JFHS.2013.28.4.367
  3. Kim YB, Hong DH, Cho ES, Lm WJ, Kim IH, and Son HY (2014) Effect of repeated Paecilomyces japonica treatment on rats. Korean J Vet Res 54, 31-8. https://doi.org/10.14405/kjvr.2014.54.1.31
  4. Lee JE, Kim HJ, Choi EK, Chai HY, Yun YW, Kim DJ et al. (2003) Fourweek repeated-dose toxicity study on Pinellia Extract. Korean J Lab Anim Sci 19, 127-41.
  5. Lee JS, Lee JS, Park YC, Choi SM, and Lee SH (2014) A repeated-dose 28-day oral toxicity test of Aconitum jaluense extract in sprague-dawley rats. Yakhak Hoeji 58, 62-70.
  6. Li B, Choi HJ, Lee DS, Oh H, Kim YC, Moon JY et al. (2014) Amomum tsao-ko suppresses lipopolysaccharide-induced inflammatory responses in RAW264.7 macrophages via Nrf2-dependent heme oxygenase-1 expression. Am J Chin Med 42, 1229-44. https://doi.org/10.1142/S0192415X14500773
  7. Moon SS, Cho SC, and Lee JY (2005) Tsaokoarylone, a cytotoxic diarylheptanoid from Amomum tsao-ko fruits. Bull Korean Chem Soc 26, 447-50. https://doi.org/10.5012/bkcs.2005.26.3.447
  8. Moon SS, Lee JY, and Cho SC (2004) Isotsaokoin, an antifungal agent from Amomum tsao-ko. J Nat Prod 67, 889-91. https://doi.org/10.1021/np030464l
  9. Na CS, Hong CY, Na DS, Kim JB, Yoon SY, Lee SB et al. (2013) Hotwater extract of Hovenia dulcis peduncle improves exercise performance and anti-fatigue effect in Mice. Kor J Pharmacogn 44, 83-90.
  10. Roh HS, Seok JH, Jeong JY, Lee JK, Kim TS, Choi HK et al. (2014) Repeated dose oral toxicity study of Alismatis rhizoma in SD Rats. J Korean Med Ophthalmol Otolaryngol Dermatol 27, 79-90. https://doi.org/10.6114/jkood.2014.27.1.079
  11. Um MY, Ha TY, Seong KS, and Kim YS (2013) In vitro screening of the acetylcholinesterase inhibition, anti-oxidant activity, and neuronal cell protective effect of medicinal plant extracts. Korean J Food Preserv 20, 840-5. https://doi.org/10.11002/kjfp.2013.20.6.840
  12. Yang Y, Yue Y, Runwei Y, and Guolin Z (2010) Cytotoxic, apoptotic and antioxidant activity of the essential oil of Amomum tsao-ko. Bioresour Technol 101, 4205-11. https://doi.org/10.1016/j.biortech.2009.12.131
  13. Yoo JG and Jung JY (2012) Studies on the repeated toxicity test of food red No.2 for 4 weeks oral administration in SD Rat. J Fd Hyg Safety 27, 42-9. https://doi.org/10.13103/JFHS.2012.27.1.042
  14. Yoon HJ, Choe MS, Cho HJ, Han BS, Park KH, Oh JA et al. (2014) Study of kidney toxicity of Azadirachta indica extract for oral administration in rats. Korean J Environ Agric 33, 103-10. https://doi.org/10.5338/KJEA.2014.33.2.103
  15. Yu L, Shirai N, Suzuki H, Sugane N, Hosono T, Nakajima Y et al. (2010) The effect of methanol extracts of tsao-ko (Amomum tsao-ko Crevost et Lemaire) on digestive enzyme and antioxidant activity in vitro, and plasma lipids and glucose and liver lipids in mice. J Nutr Sci Vitaminol (Tokyo) 56, 171-6. https://doi.org/10.3177/jnsv.56.171

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