Journal of Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Characterization of a Corn Fiber Protein Film Containing Green Tea Extract

녹차 추출물을 함유한 옥피 단백질 필름의 특성

  • Yang, Hyun-Ju (Department of Food Science and Technology, Chungnam National University) ;
  • Lee, Ji-Hyun (Department of Food Science and Technology, Chungnam National University) ;
  • Lee, Ji-Hyeon (Department of Food Science and Technology, Chungnam National University) ;
  • Song, Kyung Bin (Department of Food Science and Technology, Chungnam National University)
  • Received : 2015.01.12
  • Accepted : 2015.03.11
  • Published : 2015.06.30
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Abstract

Corn fiber protein (CFP) was extracted from corn wet-milling by-product, corn fiber. CFP films containing various plasticizers and cross-linking agents were prepared and their mechanical properties were determined. Among the plasticizers and cross-linking agents used in this study, the CFP film containing 2 g fructose and 0.03% cinnamaldehyde had the most appropriate physical property. In addition, the CFP films containing green tea extract (GTE) were prepared by incorporating different amounts (0, 0.5, 1.0, 1.5%) of GTE into the film-forming solution. Tensile strength, film solubility, and opacity of the CFP films increased with the addition of GTE, whereas elongation and water vapor permeability of the CFP/GTE films decreased compared to those of the control. The antioxidant activity of the CFP/GTE film was determined in terms of 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity. As a result, antioxidant activity of the films increased with increasing GTE concentration. Furthermore, antimicrobial activity against Escherichia coli O157:H7, Listeria monocytogenes, and Staphylococcus aureus increased with increasing GTE concentration. These results indicate that the incorporation of GTE could enhance antioxidant and antimicrobial activities of the CFP films.

옥수수의 습식 분쇄 공정 중 나오는 부산물인 옥피로부터 옥피 단백질(CFP)을 추출하였다. 다양한 가소제와 가교제를 첨가하여 CFP 필름을 제조한 후, 필름의 물성을 측정하였다. 본 연구에서 사용된 가소제와 가교제 중, 2 g fructose와 0.03% cinnamaldehyde를 첨가한 필름이 가장 좋은 물성을 가졌다. 또한 여러 농도(0, 0.5, 1.0, 1.5%)의 녹차 추출물(GTE)을 첨가한 CFP 필름을 제조하였다. GTE의 농도가 증가함에 따라 CFP 필름의 인장강도, 수분 용해도, 불투명도는 증가하였고, 반면에 신장률과 필름의 수분투과도는 대조구에 비해 감소하였다. GTE 첨가한 CFP 필름의 항산화성은 DPPH 소거능으로 측정하였는데, GTE 농도가 증가함에 따라 증가하였다. 그리고 E. coli O157:H7, L. monocytogenes, S. aureus 에 대한 항균성 실험을 한 결과 또한 GTE 농도가 증가함에 따라 증가하였다. 따라서 본 연구 결과, GTE 첨가는 CFP 필름의 항산화, 항균효과를 가져다 준다고 판단된다.

Keywords

References

  1. AOAC (1995) Official method of analysis. Association of Official Analytical Chemists (NO. 993.13), USA.
  2. ASTM (1983) Standard test method for water vapor transmission of material. E 96-80, Annual Book of ASTM Standard, USA.
  3. ASTM (1993) Standard test method for tensile properties of plastics. D638M, Annual Book of ASTM Standard, USA.
  4. Atef M, Rezaei M, and Behrooz R (2014) Preparation and characterization agar-based nanocomposite film reinforced by nanocrystalline cellulose. Int J Biol Macromol 70, 537-44. https://doi.org/10.1016/j.ijbiomac.2014.07.013
  5. Bandyopadhyay P, Ghosh AK, and Ghosh C (2012) Recent developments on polyphenol-protein interactions: effects on tea and coffee taste, antioxidant properties and the digestive system. Food Funct 3, 592-605. https://doi.org/10.1039/c2fo00006g
  6. Cao N, Fu Y, and He J (2007) Mechanical properties of gelatin films crosslinked, respectively, by ferulic acid and tannin acid. Food Hydrocolloid 21, 575-84. https://doi.org/10.1016/j.foodhyd.2006.07.001
  7. Chiu PE and Lai LS (2010) Antimicrobial activities of tapioca starch/decolorized hsian-tsao leaf gum coatings containing green tea extracts in fruit-based salads, romaine hearts and pork slices. Int J Food Microbiol 139, 23-30. https://doi.org/10.1016/j.ijfoodmicro.2010.01.010
  8. Galietta G, Di Gioia L, Guilbert S, and Cuq B (1998) Mechanical and thermomechanical properties of films based on whey proteins as affected by plasticizer and crosslinking agents. J Dairy Sci 81, 3123-30. https://doi.org/10.3168/jds.S0022-0302(98)75877-1
  9. Gaspar M, Kalman G, and Reczey K (2007) Corn fiber as a raw material for hemicellulose and ethanol production. Process Biochem 42, 1135-9. https://doi.org/10.1016/j.procbio.2007.04.003
  10. Gimenez B, Lopez de Lacey A, Perez-Santin E, Lopez-Caballero ME, and Montero P (2013) Release of active compounds from agar and agargelatin films with green tea extract. Food Hydrocolloid 30, 264-71. https://doi.org/10.1016/j.foodhyd.2012.05.014
  11. Hong YH, Lim GO, and Song KB (2009) Physical properties of Gelidium corneum-gelatin blend films containing grapefruit seed extract or green tea extract and its application in the packaging of pork loins. J Food Sci 74, C6-10. https://doi.org/10.1111/j.1750-3841.2008.00987.x
  12. Kim KM, Lee BY, Kim YT, Choi SG, Lee J, Cho SY et al. (2006) Development of antimicrobial edible film incorporated with green tea extract. Food Sci Biotechnol 15, 478-81.
  13. Ku K, Seo Y, and Song KB (2007) Physical properties of Gelidium corneum films treated with cinnamaldehyde. J Food Sci Nutr 12, 122-5. https://doi.org/10.3746/jfn.2007.12.2.122
  14. Kumudavally KV, Phanindrakumar HS, Tabassum A, Radhakrishna K, and Bawa AS (2008) Green tea-A potential preservative for extending the shelf life of fresh mutton at ambient temperature ($25{\pm}2^{\circ}C$). Food Chem 107, 426-33. https://doi.org/10.1016/j.foodchem.2007.08.045
  15. Laemlli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680-5. https://doi.org/10.1038/227680a0
  16. Lee JH, Song NB, Jo WS, and Song KB (2014) Effects of nanoclay type and content on the physical properties of sesame seed meal protein composite films. Int J Food Sci Technol 49, 1869-75. https://doi.org/10.1111/ijfs.12496
  17. Lee JJ and Rhim JW (2000) Characteristics of edible films based with various cultivars of sweet potato starch. Korean J Food Sci Technol 32, 834-42.
  18. Lee MS, Lee SH, Park SK, Bae DH, Ha SD, and Song KB (2004) Change in quality of Pine Nuts (Pinus koraiensis) and Walnuts (Juglans regia) coated with protein film containing green tea extract during storage. Korean J Food Sci Technol 36, 842-6.
  19. Lim GO, Hong YH, and Song KB (2009) Effect of various plasticizers and ferulic acid on the physical properties of Gelidium corneum film. J Korean Soc Food Sci Nutr 38, 727-31. https://doi.org/10.3746/jkfn.2009.38.6.727
  20. Lim GO, Jang SA, Kim JY, Kim HJ, and Song KB (2010) Use of a gelatin film containing grapefruit seed extract in the packaging of strawberries. Korean J Food Preserv 17, 196-201.
  21. Ludevid MD, Torrent M, Martinez-Izquierdo JA, Puigdomenech P, and Palau J (1984) Subcellular localization of glutelin-2 in maize (Zea mays L.) endosperm. Plant Mol Biol 3, 227-34. https://doi.org/10.1007/BF00029658
  22. Mathew S and Abraham TE (2006) Studies on the antioxidant activities of cinnamon (Cinnamomum verum) bark extracts, through various in vitro models. Food Chem 94, 520-8. https://doi.org/10.1016/j.foodchem.2004.11.043
  23. Mathew S and Abraham TE (2008) Characterisation of ferulic acid incorporated starch-chitosan blend films. Food Hydrocolloid 22, 826-35. https://doi.org/10.1016/j.foodhyd.2007.03.012
  24. Nouri L and Mohammadi Nafchi A (2014) Antibacterial, mechanical, and barrier properties of sago starch film incorporated with betel leaves extract. Int J Biol Macromol 66, 254-9. https://doi.org/10.1016/j.ijbiomac.2014.02.044
  25. Ojagh SM, Rezaei M, Razavi SH, and Hosseini SMH (2010) Development and evaluation of a novel biodegradable film made from chitosan and cinnamon essential oil with low affinity toward water. Food Chem 122, 161-6. https://doi.org/10.1016/j.foodchem.2010.02.033
  26. Rojas-Graü MA, Avena-Bustillos RJ, Friedman M, Henika PR, Martin-Belloso O, and McHugh TH (2006) Mechanical, barrier, and antimicrobial properties of apple puree edible films containing plant essential oils. J Agric Food Chem 54, 9262-7. https://doi.org/10.1021/jf061717u
  27. Rose DJ, Inglett GE, and Liu SX (2010) Utilisation of corn (Zea mays) bran and corn fiber in the production of food components. J Sci Food Agric 90, 915-24.
  28. Shin YJ, Jang SA, and Song KB (2011) Preparation and mechanical properties of rice bran protein composite films containing gelatin or red algae. Food Sci Biotechnol 20, 703-7. https://doi.org/10.1007/s10068-011-0099-1
  29. Shin YJ, Song HY, Jo WS, Lee MJ, and Song KB (2013) Physical properties of a barley protein/nanoclay composite film containing grapefruit seed extract and antimicrobial benefits for packaging of Agaricus bisporus. Int J Food Sci Technol 48, 1736-43. https://doi.org/10.1111/ijfs.12145
  30. Shukla R and Cheryan M (2001) Zein: the industrial protein from corn. Ind Crops Prod 13, 171-92. https://doi.org/10.1016/S0926-6690(00)00064-9
  31. Song HY, Jo WS, Song NB, and Song KB (2012). Preparation of defatted grape seed meal protein composite films. J Korean Soc Food Sci Nutr 41, 1274-80. https://doi.org/10.3746/jkfn.2012.41.9.1274
  32. Song NB, Song HY, Jo WS, and Song KB (2013) Physical properties of a composite film containing sunflower seed meal protein and its application in packaging smoked duck meat. J Food Eng 116, 789-95. https://doi.org/10.1016/j.jfoodeng.2013.02.002
  33. Wang B, Cheng B, and Feng H (2008) Enriched arabinoxylan in corn fiber for value-added products. Biotechnol Lett 30, 275-9. https://doi.org/10.1007/s10529-007-9537-9
  34. Wang L, Dong Y, Men H, Tong J, and Zhou J (2013) Preparation and characterization of active films based on chitosan incorporated tea polyphenols. Food hydrocolloid 32, 35-41. https://doi.org/10.1016/j.foodhyd.2012.11.034
  35. Wu J, Chen S, Ge S, Miao J, Li J, and Zhang Q (2013) Preparation, properties and antioxidant activity of an active film from silver carp (Hypophthalmichthys molitrix) skin gelatin incorporated with green tea extract. Food Hydrocolloid 32, 42-51. https://doi.org/10.1016/j.foodhyd.2012.11.029

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